Skip to main content

Advertisement

SpringerLink
Log in

Characterization and mapping of R Pi-ber , a novel potato late blight resistance gene from Solanum berthaultii

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Phytophthora infestans, the causal agent of late blight, threatens potato production worldwide. An important tool in the management of the disease is the use of resistant varieties. Eleven major resistance genes have been identified and introgressed from Solanum demissum. However, new sources of resistance are continually sought. Here, we report the characterization and refined genetic localization of a resistance gene previously identified as Rber in a backcross progeny of Solanum tuberosum and Solanum berthaultii. In order to further characterize Rber, we developed a set of P. infestans isolates capable of identifying each of the 11 R-genes known to confer resistance to late blight in potato. Our results indicate that Rber is a new resistance gene, different from those recognized in S. demissum, and therefore, it has been named R Pi-ber according to the current system of nomenclature. In order to add new molecular markers around R Pi-ber , we used a PCR-based mapping technique, named MASP-map, which located R Pi-ber in a 3.9 cM interval between markers CT240 and TG63 on potato chromosome X. The location of R Pi-ber coincides with an area involved in resistance to different pathogens of potato and tomato.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

AUDPC:

Area under the disease progress curve

BCT:

Backcross to S. tuberosum progeny

BSA:

Bulk segregant analysis

CAPS:

Cleaved amplified polymorphic sequences

MASP-map:

Multiplex allele-specific PCR mapping

R-gene:

Resistance gene

chr:

Chromosome

RFLP:

Restriction fragment length polymorphisms

References

  • Abu-El Samen FM, Secor GA, Gudmestad NC (2003) Variability in virulence among asexual progenies of Phytophthora infestans. Phytopathology 93:293–304

    Article  CAS  Google Scholar 

  • Ballvora A, Ercolano MR, WeiB J, Meksem K, Bormann CA, Oberhagemann P, Salamini F, Gebhardt C (2002) The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes. Plant J 30:361–371

    Article  PubMed  CAS  Google Scholar 

  • Black W, Mastenbroek C, Mills WR, Peterson LC (1953) A proposal for an international nomenclature of races of Phytophthora infestans and genes controlling immunity in Solanum demissum derivatives. Euphytica 2:173–179

    Article  Google Scholar 

  • Bonierbale MW, Plaisted RL, Pineda O, Tanksley SD (1994) QTL analysis of trichome-mediated insect resistance in potato. Theor Appl Genet 87:973–987

    Article  CAS  Google Scholar 

  • Bonierbale MW, Plaisted RL, Tanksley SD (1988a) Construction of a genetic map of potato based on molecular markers from tomato. Am J Potato Res.65:471–472

    Google Scholar 

  • Bonierbale MW, Plaisted RL, Tanksley SD (1988b) RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120:1095–1103

    Google Scholar 

  • Bormann CA, Rickert AM, Castillo Ruiz RA, Paal J, Lübeck J, Strahwald J, Buhr K, Gebhardt C (2004) Tagging quantitative trait loci for maturity-corrected late blight resistance in tetraploid potato with PCR-based candidate gene markers. Mol Plant-Microbe Interact 17:1126–1139

    Article  PubMed  CAS  Google Scholar 

  • Caten CE, Jinks. JL (1968) Spontaneous variability of single isolates of Phytophthora infestans. I. Cultural variation. Can J Bot 46:329–348

    Article  Google Scholar 

  • Cha RS, Zarbl H, Keohavong P, G.Thilly W (1992) Mismatch amplification mutation assay (MAMA): application to the c-H-ras gene. PCR Methods Appl 2:14–20

    PubMed  CAS  Google Scholar 

  • Chen XM, Line RF, Leung H (1998) Genome scanning for resistance-gene analogs in rice, barley and wheat by high resolution electrophoresis. Theor Appl Genet 97:345–355

    Article  CAS  Google Scholar 

  • Chunwongse J, Chunwongse C, Black L, Hanson P (2002) Molecular mapping of the Ph-3 gene for late blight resistance in tomato. J Horticult Sci Biotechnol 77:281–286

    CAS  Google Scholar 

  • Clewley JP, Arnold C (1997) MEGALIGN. The multiple alignment module of LASERGENE. Methods Mol Biol 70:119–129

    PubMed  CAS  Google Scholar 

  • Deahl KL, Inglis DA, Demuth SP (1993) Testing for resistance to metalaxyl in Phytophthora infestans isolates from northwestern Washington. Am J Potato Res 70:779–795

    CAS  Google Scholar 

  • Dorrance AE, Inglis DA (1997) Assessment of greenhouse and laboratory screening methods for evaluating potato foliage for resistance to late blight. Plant Dis 81:1206–1213

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • El-Kharbotly A, Palomino-Sanchez C, Salamini F, Jacobsen E, Gebhardt C (1996) R6 and R7 alleles of potato conferring race-specific resistance to Phytophthora infestans (Mont.) de Bary identified genetic loci clustering with the R3 locus on chromosome XI. Theor Appl Genet 92:880–884

    Article  CAS  Google Scholar 

  • Ewing EE, Simko I, Smart CD, Bonierbale MW, Mizubuti ESG, May GD, Fry WE (2000) Genetic mapping of qualitative and quantitative field resistance to Phytophthora infestans in a population derived from Solanum tuberosum and Solanum berthaultii. Mol Breed 6:25–36

    Article  CAS  Google Scholar 

  • Fry WE, Goodwin SB (1997) Resurgence of the Irish potato famine fungus. BioScience 47:363–371

    Article  Google Scholar 

  • Ganal MW, Czihal R, Hannapel U, Kloos D, Polley A, Ling H-Q (1998) Sequencing cDNA clones from the genetic map of tomato (Lycopersicon esculentum). Genome Res 8:842–847

    PubMed  CAS  Google Scholar 

  • Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F (1991) RFLP maps of potato and their alignment with the homoeologus tomato genome. Theor Appl Genet 83:49–57

    Article  Google Scholar 

  • Gebhardt C, Valkonen JPT (2001) Organization of genes controlling disease resistance in the potato genome. Annu Rev Phytopathol 39:79–102

    Article  PubMed  CAS  Google Scholar 

  • GILB (2004) Global initiative on late blight, http://www.cipotato.org/gilb/

  • Goodwin SB, Sujkowski LS, Fry WE (1996) Widespread distribution and probable origin of resistance to metalaxyl in clonal genotypes of Phytophthora infestans in the United States and Western Canada. Phytopathology 86:793–800

    Article  CAS  Google Scholar 

  • Grube RC, Radwanski ER, Jahn M (2000) Comparative genetics of disease resistance within the Solanaceae. Genetics 155:873–887

    PubMed  CAS  Google Scholar 

  • Grunwald NJ, Flier WG, Sturbaum AK, Garay-Serrano E, van den Bosch TBM, Smart CD, Matuszak JM, Lozoya-Saldana H, Turkensteen LJ, Fry WE (2001) Population structure of Phytophthora infestans in the Toluca valley region of central Mexico. Phytopathology 91:882–890

    Article  PubMed  CAS  Google Scholar 

  • Helgeson JP, Pohlman JD, Austin S, Haberlach GT, Wielgus SM, Ronis D, Zambolim L, Tooley P, McGrath JM, James RV, Stevenson WR (1998) Somatic hybrids between Solanum bulbocastanum and potato: a new source of resistance to late blight. Theor Appl Genet 96:738–742

    Article  Google Scholar 

  • Huang S (2005) High allelic diversity of the potato R3 complex locus suggests a novel strategy for late blight control. Ph. D Thesis. Wageningen University, The Netherlands

  • Huang S, van der Vossen EAG, Kuang H, Vleeshouwers VGAA, Zhang N, Borm THA, van Eck H, Baker BJ, Jacobsen E, Visser RGF (2005) Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato. Plant J (in press)

  • Huang SW, Vleeshouwers V, Werij JS, Hutten RCB, van Eck HJ, Visser RGF, Jacobsen E (2004) The R3 resistance to Phytophthora infestans in potato is conferred by two closely linked R genes with distinct specificities. Mol Plant-Microbe Interact 17:428–435

    Article  PubMed  CAS  Google Scholar 

  • Kanazin V, Marek LF, Shoemaker RC (1996) Resistance gene analogs are conserved and clustered in soybean. Proc Natl Acad Sci USA 93:11746–11750

    Article  PubMed  CAS  Google Scholar 

  • Konieczny A, Ausubel FM (1993) A procedure for mapping Arabidopsis mutations using codominant ecotype-specific PCR-based markers. Plant J 4:403–410

    Article  PubMed  CAS  Google Scholar 

  • Kuhl JC, Hanneman RE, Havey MJ (2001) Characterization and mapping of Rpi1, a late blight resistance locus from diploid (1EBN) Mexican Solanum pinnatisectum. Mol Genet Genom 265:977–985

    Article  CAS  Google Scholar 

  • Leister D, Ballvora A, Salamini F, Gebhardt C (1996) A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants. Nat Genet 14:421–429

    Article  PubMed  CAS  Google Scholar 

  • Leister D, Berger A, Thelen H, Lehmann W, Salamini F, Gebhardt C (1997) Construction of potato YAC library and identification of clones linked to the disease resistance loci R1 and Gro1. Theor Appl Genet 95:954–960

    Article  CAS  Google Scholar 

  • Leonards-Schippers C, Gieffers W, Salamini F, Gebhardt C (1992) The R1 gene conferring race-specific resistance to Phytophthora infestans in potato is located on potato chromosome V. Mol Gen Genet 233:278–283

    Article  PubMed  CAS  Google Scholar 

  • Leonards-Schippers C, Gieffers W, Schafer-Pregl R, Ritter E, Knapp S, Salamini F, Gebhardt C (1994) Quantitative resistance to Phytophthora infestans in potato: a case study for QTL mapping in an allogamous plant species. Genetics 137:67–77

    PubMed  CAS  Google Scholar 

  • Li X, van Eck HJ, Rouppe van der Voort JNAM, Huigen DJ, Stam P, Jacobsen E (1998) Autotetraploids and genetic mapping using common AFLP markers: the R2 allele conferring resistance to Phytophthora infestans mapped on potato chromosome 4. Theor Appl Genet 96:1121–1128

    Article  CAS  Google Scholar 

  • Malcomson JF, Black W (1966) New R-genes in Solanum demissum Lindl. and their complementary races of Phytophthora infestans (Mont) de Bary. Euphytica 15:199–203

    Article  Google Scholar 

  • Manly KF, Cudmore JRH, Meer JM (2001) Map manager QTX, cross platform software for genetic mapping. Mamm Genome 12:930–932

    Article  PubMed  CAS  Google Scholar 

  • Mastenbroek C (1952) Investigations into the inheritance of the immunity from Phytophthora infestans De B. of Solanum demissum Lindl. Euphytica 1:187–198

    Article  Google Scholar 

  • Meksem K, Leister D, Peleman J, Zabeau M, Salamini F, Gebhardt C (1995) A high resolution map of the vicinity of the R1 locus on chromosome V of potato based on RFLP and AFLP markers. Mol Gen Genet 249:74–81

    Article  PubMed  CAS  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulk segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  Google Scholar 

  • Milbourne D, Meyer RC, Collins AJ, Ramsay LD, Gebhardt C, Waugh R (1998) Isolation, characterization and mapping of simple sequence repeat loci in potato. Mol Gen Genet 259:233–245

    Article  PubMed  CAS  Google Scholar 

  • Moreau P, Thoquet P, Olivier J, Laterrot H, Grimsley N (1998) Genetic mapping of Ph-2, a single locus controlling partial resistance to Phytophthora infestans in tomato. Mol Plant-Microbe Interact 11:259–269

    Article  CAS  Google Scholar 

  • Naess SK, Bardeen JM, Wielgus SM, Haberlach GT, McGrath JM, Hegelson JP (2000) Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8. Theor Appl Genet 295:1–8

    Google Scholar 

  • Okimoto R, Dogson JB (1996) Improved PCR amplification of multiple specific alleles (PAMSA) using internally mismatched primers. Biotechniques 21:20–26

    PubMed  CAS  Google Scholar 

  • Paterson AH (1996) Making genetic maps. In: Paterson AH (eds) Genome mapping in plants. Academic, Austin pp 22–39

    Google Scholar 

  • Rivera-Peña A (1990) Wild tuber-bearing species of Solanum and incidence of Phythophthora infestans (Mont.) de Bary on the western slopes of the volcano Nevado de Toluca. 5. Type of resistance to P. infestans. Potato Res 33:479–486

    Article  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Plainview

    Google Scholar 

  • Schick R, Schick E, Haussdorfer M (1958) Ein beitrag zur physiologischen spezialisierung von Phytophthora infestans. Phytopath Z 31:225–236

    Google Scholar 

  • Shaner G, Finney RE (1977) The effect of nitrogen fertilization on the expression of slow-mildewing resistance in Knowx wheat. Phytopathology 67:1051–1056

    CAS  Google Scholar 

  • Simko I (2002) Comparative analysis of quantitative trait loci for foliage resistance to Phytophthora infestans in tuber-bearing Solanum species. Am J Potato Res 79:125–132

    CAS  Google Scholar 

  • Solomon-Blackburn RM, Barker H (2001) A review of host major-gene resistance to potato viruses X, Y, A and V in potato: genes, genetics and mapped locations. Heredity 86:8–16

    Article  PubMed  CAS  Google Scholar 

  • Song J, Bradeen JM, Naess SK, Raasch JA, Wielgus SM, Haberlach GT, Liu J, Kuang H, Austin-Phillips S, Buell CR, Helgeson JP, Jiang J (2003) Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight. Proc Natl Acad Sci USA 100:9128–9133

    Article  PubMed  CAS  Google Scholar 

  • Spielman LJ, McMaster BJ, Fry WE (1989) Dominance and recessiveness at loci for virulence against potato and tomato in Phytophthora infestans. Theor Appl Genet 77:832–838

    Article  Google Scholar 

  • Stewart HE, Bradshaw JE, Pande B (2003) The effect of the presence of R-genes for resistance to late blight (Phytophthora infestans) of potato (Solanum tuberosum) on the underlying level of field resistance. Plant Pathol 52:193–198

    Article  Google Scholar 

  • Tanksley SD, Ganal MW, Prince JP, Vicente MCd, Bonierbale MW, Broun P, Fulton TM, Giovanoni JJ, Grandillo S, Martin GB, Messenger R, Miller JC, Miller L, Paterson AH, Pineda O, Roder MS, Wing RA, Wu W, Young ND (1992) High density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160

    PubMed  CAS  Google Scholar 

  • Tooley PW, Sweigard JA, Fry WE (1986) Fitness and virulence of Phytophthora infestans isolates from sexual and asexual populations. Phytopathology 76:1209–1212

    Google Scholar 

  • Umaerus V, Umaerus M (1994) Inheritance of resistance to late blight. In: Bradshaw JE, Mackay GR (eds) Potato genetics. CAB International, Oxon pp 365–401

    Google Scholar 

  • USDA and ARS (1999) National genetic resources program. Germplasm Resources information network (GRIN). On-line database National Germplasm Resources Laboratory, Beltsville, Maryland. Available http://www.ars-grin.gov/npgs/searchgrin.html

  • Van der Plank JE (1963) Plant diseases: epidemics and control. New York

  • Wastie RL (1991) Breeding for resistance. Adv Plant Pathol. 7:193–223

    Google Scholar 

  • Ye S, Dhillon S, Ke X, Collins AR, Day INM (2001) An efficient procedure for genotyping single nucleotide polymorphisms. Nucl Acids Res 29:e88

    Article  PubMed  CAS  Google Scholar 

  • Yu YG, Buss GR, Maroof MAS (1996) Isolation of a superfamily of candidate disease-resistance genes in soybean based on a conserved nucleotide binding site. Proc Natl Acad Sci USA 93:11751–11756

    Article  PubMed  CAS  Google Scholar 

  • Zhang LP, Khan AA, Niño-Liu D, Foolad MR (2002) A molecular linkage map of tomato displaying chromosomal locations of resistance gene analogs based on a Lycopersicon esculentum × Lycopersicon hirsutum cross. Genome 45:133–146

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

The present study was supported by Syngenta. We are grateful to Jason Rauscher, for critical reading and helpful suggestions on the manuscript, and to Kevin Myers and Barbara Esposito for their skilled technical assistance. RFLP markers were kindly provided by S. Tanksley.

Author information

Author notes

  1. I. Simko

    Present address: USDA-ARS, Beltsville, MD, USA

  2. C. D. Smart

    Present address: Department of Plant Pathology, Cornell University, Geneva, NY, USA

  3. A. Greenland

    Present address: National Institute for Agricultural Botany, Cambridge, UK

Authors and Affiliations

  1. Department of Plant Pathology, Cornell University, Ithaca, NY, USA

    G. M. Rauscher, C. D. Smart, H. Mayton & W. E. Fry

  2. Department of Horticulture, Cornell University, Ithaca, NY, USA

    I. Simko

  3. Centro Internacional de la Papa, Lima, Peru

    M. Bonierbale

  4. Syngenta Jealott’s Hill, Bracknell, UK

    A. Greenland

Authors
  1. G. M. Rauscher
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. D. Smart
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. Simko
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Bonierbale
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Mayton
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Greenland
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. W. E. Fry
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to W. E. Fry.

Additional information

Communicated by G. Wenzel

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rauscher, G.M., Smart, C.D., Simko, I. et al. Characterization and mapping of R Pi-ber , a novel potato late blight resistance gene from Solanum berthaultii . Theor Appl Genet 112, 674–687 (2006). https://doi.org/10.1007/s00122-005-0171-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-005-0171-4

Keywords

Search

Navigation