Skip to main content

Development and characterization of wheat- Leymus racemosus translocation lines with resistance to Fusarium Head Blight


Wheat scab (Fusarium Head Blight, FHB) is a destructive disease in the warm and humid wheat-growing areas of the world. Finding diverse sources of FHB resistance is critical for genetic diversity of resistance for wheat breeding programs. Leymus racemosus is a wild perennial relative of wheat and is highly resistant to FHB. Three wheat- L. racemosus disomic addition (DA) lines DA5Lr#1, DA7Lr#1 and DALr.7 resistant to FHB were used to develop wheat- L.racemosus translocation lines through irradiation and gametocidal gene-induced chromosome breakage. A total of nine wheat-alien translocation lines with wheat scab resistance were identified by chromosome C-banding, GISH, telosomic pairing and RFLP analyses. In line NAU614, the long arm of 5Lr#1 was translocated to wheat chromosome 6B. Four lines, NAU601, NAU615, NAU617, and NAU635, had a part of the short arm of 7Lr#1 transferred to different wheat chromosomes. Four other lines, NAU611, NAU634, NAU633, and NAU618, contained translocations involving Leymus chromosome Lr.7 and different wheat chromosomes. The resistance level of the translocation lines with a single alien chromosome segment was higher than the susceptible wheat parent Chinese Spring but lower than the alien resistant parent L. racemosus. At least three resistance genes in L. racemosus were identified. One was located on chromosome Lr.7, and two could be assigned to the long arm of 5Lr#1 and the short arm of 7Lr#1.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3


  • Chen PD, Wang ZT, Wang SL, Huang L, Wang YZ, Liu DJ (1993) Transfer of scab resistance from Elymus giganteus into common wheat. In: Li ZS, Xin ZY (eds) Proceedings of the 8th international wheat genetics symposium. China Agricultural Scientech Press, Beijing 1:153–157

  • Chen PD, Wang ZT, Wang SL, Huang L, Wang YZ, Liu DJ (1995) Transfer of useful germplasm from Leymus racemosus Lam. to common wheat. III. Development of addition lines with wheat scab resistance (in Chinese with English abstract). Acta Genet Sin 22:206–210

    Google Scholar 

  • Chen PD, Sun WX, Liu WX, Yuan JH, Liu ZH, Wang SL, Liu DJ (1998) Development of wheat- Leymus racemosus translocation lines with scab resistance. In: Slinkard AE (ed) Proceedings of the 9th international wheat genetics symposium. University Extension Press, Saskatoon, Canada 2:32–34

  • Endo TR (1988) Induction of chromosomal structural changes by a chromosome of Aegilops cylindrica in common wheat. J Hered 79:366–370

    Google Scholar 

  • Endo TR (1994) Structural changes of rye chromosome 1R induced by gametocidal chromosome. Jpn J Genet 69:11–19

    Article  Google Scholar 

  • Gill BS, Friebe B, Endo TR (1991) Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat. Genome 34:830–839

    Google Scholar 

  • Jiang JM, Gill BS (1994) Different species-specific chromosome translocations in Triticum timopheevii and T. turgidum support the diphyletic origin of polyploid wheat. Chromosome Res 2:59–64

    Article  PubMed  CAS  Google Scholar 

  • Liu DJ (2002) Genome analysis in wheat breeding for disease resistance. Acta Bot Sin 44:1096–1104

    CAS  Google Scholar 

  • Liu WX, Chen PD, Liu DJ (1999) Development of Triticum aestivum Leymus racemosustranslocation lines by irradiating adult plants at meiosis (in Chinese with English abstract). Acta Bot Sin 41(5):463–467

    Google Scholar 

  • Liu WX, Chen PD, Liu DJ (2000a) Studies of the development of Triticum aestivum Leymus racemosustranslocation lines by pollen irradiation (in Chinese with English abstract). Acta Genet Sin 27:40–49

    CAS  Google Scholar 

  • Liu WX, Chen PD, Liu DJ (2000b) Selection, breeding and identification of T01- T.aestivum-L.racemosus translocation line (in Chinese with English abstract). Acta Agron Sin 26:305–309

    Google Scholar 

  • Mujeeb-Kazi A, Bernard M, Bekele GT, Mirand JL (1983) Incorporation of alien genetic information from Elymus giganteus into Triticum aestivum. In: Sakamoto S (ed) Proceedings of the 6th international wheat genetics symposium. Plant germ-plasm Institute, Kyoto, Japan pp 223–231

  • Nasuda S, Friebe B, Gill BS (1998) Gametocidal genes induce chromosome breakage in the interphase prior to the first mitotic cell division of the male gametophyte in wheat. Genetics 149:1115–1124

    PubMed  CAS  Google Scholar 

  • Qi LL, Wang SL, Chen PD, Liu DJ, Friebe B, Gill BS (1997) Molecular cytogenetic analysis of Leymus racemosus chromosomes added to wheat. Theor Appl Genet 95:1084–1091

    Article  CAS  Google Scholar 

  • Riley R, Chapman V, Johnson R (1968) Introduction of yellow rust resistance of Aegilops comosa into wheat by genetically induced homoeologous recombination. Nature 217:383–384

    Article  Google Scholar 

  • Sears ER (1956) The transfer of leaf rust resistance from Aegilops umbellulata to wheat. Brookhaven Symp Biol 9:1–21

    Google Scholar 

  • Sears ER (1972) Chromosome engineering in wheat. Stadler Symp 4:23–38

    Google Scholar 

  • Sears ER (1993) Use of radiation to transfer alien segments to wheat. Crop Sci 33:897–901

    Article  Google Scholar 

  • Sharp PJ, Chao S, Desai S and Gale MD (1989) The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm. Theor Appl Genet 78:342–348

    Article  Google Scholar 

  • Wang XE, Chen PD, Zhou B, Yuan JH, Liu WX, Gill BS, Liu DJ (2001) RFLP analysis of wheat- L. racemosus translocation lines (in Chinese with English abstract). Acta Genet Sin 28:1142–1150

    PubMed  CAS  Google Scholar 

  • Wang YN, Chen PD, Liu DJ (1986) Transfer of useful germplasm from Elymus giganteus L. to common wheat. I. Production of (T.aestivum cv. Chinese Spring- Elymus giganteus) F1 (in Chinese with English abstract). J Nanjing Agric Univ 9:10–14

    Google Scholar 

  • Wang YZ, Yang XN, Xiao CP (1982) The improvement of identification technique for wheat scab resistance and development of resistant sources (in Chinese). Sci Agric Sin 5:67–77

    Google Scholar 

  • Wen YQ, Liu DJ (1989) Morphology, scab resistance and cytogenetics of intergeneric hybrids of Triticum aestivum L. with Roegneria C. Koch (Agropyron) species (in Chinese with English abstract). Scentia Agric Sin 22:1–7

    Google Scholar 

  • Yang BJ, Dou QW, Liu WX, Zhou B, Chen PD (2002) Development of Triticum aestivum-Leymus racemosus translocation lines NAU601 and NAU618 and their test-cross analysis with double ditelosomic (in Chinese with English abstract). Acta Genet Sin 29:350–354

    PubMed  CAS  Google Scholar 

  • Yuan JH, Chen PD, Liu DJ (2003) Development of Triticum aestivum Leymus racemosus translocation lines using gametocidal chromosomes. Science in China 46:522–532

    Article  CAS  PubMed  Google Scholar 

  • Zhang P, Friebe B, Gill BS (2002) Variation in the distribution of a genome-specific DNA sequence on the chromosomes reveals evolutionary relationships in the Triticum and Aegilops complex. Plant Syst Evol 235:169–179

    Article  CAS  Google Scholar 

Download references


We thank Dr. Endo, Kyoto University, Japan, for providing seeds of wheat- Ae.cylindrica addition line; the Jiangsu Academy of Agricultural Sciences for irradiation treatment and supplying the pathogen isolates of Fusarium graminearum; Jianyang Institute of Agricultural Sciences, Fujian province for identification of the scab resistance; and John Raupp and Duane Wilson for research assistance. The project was supported by the National Natural Sciences of China, the Jiangsu Natural Science Foundation, Education Ministry of China and McKnight Foundation (USA) CCRP grant to Nanjing Agricultural University and Kansas State University.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Peidu Chen.

Additional information

Communicated by C. Möllers

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Chen, P., Liu, W., Yuan, J. et al. Development and characterization of wheat- Leymus racemosus translocation lines with resistance to Fusarium Head Blight. Theor Appl Genet 111, 941–948 (2005).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:


  • Wheat Chromosome
  • Fusarium Head Blight Resistance
  • Translocation Line
  • Scab Resistance
  • Alien Chromosome