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Mapping of QTL associated with chilling tolerance during reproductive growth in soybean

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Abstract

Low temperatures in summer bring about drastic reduction in seed yield of soybean [Glycine max (L.) Merr.]. To identify quantitative trait loci (QTL) associated with chilling tolerance during the reproductive growth in soybean, a recombinant inbred line (RIL) population consisting of 104 F6-derived lines was created from a cross between two cultivars, chilling-tolerant Hayahikari and chilling-sensitive Toyomusume. The RIL were genotyped with 181 molecular and phenotypic markers and were scored with regard to chilling tolerance, which was evaluated by comparison of seed-yielding abilities in two artificial climatic environments at chilling and usual temperatures. Three QTL were detected for chilling tolerance in seed-yielding ability. Two of them, qCTTSW1 and qCTTSW2, were mapped near QTL for flowering time, and the latter had an epistatic interaction with a marker locus located near another QTL for flowering time, where no significant QTL for chilling tolerance was detected. The analysis of an F2 population derived from the cross between Hayahikari and an RIL of the Hayahikari genotype at all QTL for flowering time confirmed the effect of the third QTL, qCTTSW3, on chilling tolerance and suggested that qCTTSW1 was basically independent of the QTL for flowering time. The findings and QTL found in this study may provide useful information for marker-assisted selection (MAS) and further genetic studies on soybean chilling tolerance.

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Acknowledgements

The authors wish to thank Drs. S. Shirai, S. Ohnishi and the staff of the Soybean Breeding Laboratory, Tokachi Agric. Exp. Stn. and Dr. H. Kurosaki, Kitami Agric. Exp. Stn. for their valuable suggestions and helpful discussion. The technical assistance of R. Narita, S. Sudo, K. Wada and R. Sugisawa is gratefully acknowledged. We are also grateful to Dr. H. Yamauchi (NARCH) for his encouragement. This work was supported by the Rice Genome Research Program (Section: Development of DNA Marker-aided Selection Technology for Plants and Animals) of the Ministry of Agriculture, Forestry and Fishery of Japan.

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  1. K. Kawaguchi

    Present address: Ministry of Agriculture, Forestry and Fisheries, Kasumigaseki, Tokyo, 100-8950, Japan

Authors and Affiliations

  1. National Agricultural Research Center for Hokkaido Region (NARCH), Hitsujigaoka, Toyohira-Ku, Sapporo, 062-8555, Japan

    H. Funatsuki, K. Kawaguchi, S. Matsuba, Y. Sato & M. Ishimoto

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  1. H. Funatsuki
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  2. K. Kawaguchi
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  3. S. Matsuba
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  4. Y. Sato
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Correspondence to H. Funatsuki.

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Communicated by R. Bernardo

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Funatsuki, H., Kawaguchi, K., Matsuba, S. et al. Mapping of QTL associated with chilling tolerance during reproductive growth in soybean. Theor Appl Genet 111, 851–861 (2005). https://doi.org/10.1007/s00122-005-0007-2

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  • DOI: https://doi.org/10.1007/s00122-005-0007-2

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