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Identification and characterization of a QTL on chromosome 2 for cytosolic glutamine synthetase content and panicle number in rice

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Abstract

A quantitative trait locus (QTL) associated with the protein content of cytosolic glutamine synthetase (GS1; EC 6.3.1.2) in senescing leaves, panicle number, and panicle weight was characterized in rice (Oryza sativa L.). A near-isogenic line (NIL), C-22, developed by marker-assisted selection was grown under different nitrogen levels in the greenhouse and in a paddy field. Chromosome 2 of C-22 had an approximately 50-cM segment substituted from the Kasalath (indica) chromosome in a Koshihikari (japonica) genetic background. C-22 showed a 12–37% lower content of GS1 protein in leaf blades than Koshihikari, which was in good agreement with a QTL region positively affected by the japonica chromosome. At an early vegetative stage, C-22 had more active tillers than Koshihikari in the greenhouse. At the reproductive stage, both panicle number and total panicle weight of C-22 were significantly higher than those of Koshihikari, particularly when the plants were grown under a low-nitrogen condition. These traits of C-22 were further confirmed in a paddy field. Thus, tiller development was positively affected by the Kasalath chromosome at an early vegetative stage, which resulted in an increased panicle number and panicle weight at the mature stage in C-22. These data indicate that the target QTL (Pnn1; panicle number 1) is important in the development of tillers and panicles in rice. Linkage analyses for panicle number and ratio of developing tiller formation in the second axil (RDT) revealed that Pnn1 was delimited at the 6.7-cM region.

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Acknowledgements

This work was supported in part by a program of CREST of JST (Japan Science Technology), in part by Grants-in-Aid (Nos. 10556075, 12460029, 13460144, 14360035, 14654160, and 15380003) for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and in part by Rice Genome Project 1016 (IP6001) and Genome Resource Project GE1004, Ministry of Agriculture, Forestry and Fisheries of Japan. M.O was supported by a Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists.

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Authors and Affiliations

  1. Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidoori-Amamiyamachi, Aoba-ku, Sendai, 981-8555, Japan

    Mitsuhiro Obara, Shohei Sasaki, Kenji Kashiba, Atsushi Nagano & Tomoyuki Yamaya

  2. Research Fellow of the Japan Society for the Promotion of Science, 6 Ichibancho, Chiyoda, 102-8471, Japan

    Mitsuhiro Obara

  3. Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, 981-8577, Japan

    Tadashi Sato

  4. Graduate School of Science and Technology, Chiba University, 648 Matsudo, Chiba, 271-8510, Japan

    Ikuo Nakamura

  5. Toyama Agricultural Research Center, 1124-1 Yoshioka, Toyama, 939-8153, Japan

    Takeshi Ebitani

  6. Department of Molecular Genetics, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Masahiro Yano

  7. Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan

    Tomoyuki Yamaya

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  1. Mitsuhiro Obara
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  2. Tadashi Sato
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Correspondence to Tadashi Sato.

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Communicated by D.J. Mackill

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Obara, M., Sato, T., Sasaki, S. et al. Identification and characterization of a QTL on chromosome 2 for cytosolic glutamine synthetase content and panicle number in rice. Theor Appl Genet 110, 1–11 (2004). https://doi.org/10.1007/s00122-004-1828-0

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  • DOI: https://doi.org/10.1007/s00122-004-1828-0

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