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An integrated genetic map and a new set of simple sequence repeat markers for pearl millet, Pennisetum glaucum

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Abstract

Over the past 10 years, resources have been established for the genetic analysis of pearl millet, Pennisetum glaucum (L.) R. Br., an important staple crop of the semi-arid regions of India and Africa. Among these resources are detailed genetic maps containing both homologous and heterologous restriction fragment length polymorphism (RFLP) markers, and simple sequence repeats (SSRs). Genetic maps produced in four different crosses have been integrated to develop a consensus map of 353 RFLP and 65 SSR markers. Some 85% of the markers are clustered and occupy less than a third of the total map length. This phenomenon is independent of the cross. Our data suggest that extreme localization of recombination toward the chromosome ends, resulting in gaps on the genetic map of 30 cM or more in the distal regions, is typical for pearl millet. The unequal distribution of recombination has consequences for the transfer of genes controlling important agronomic traits from donor to elite pearl millet germplasm. The paper also describes the generation of 44 SSR markers from a (CA)n-enriched small-insert genomic library. Previously, pearl millet SSRs had been generated from BAC clones, and the relative merits of both methodologies are discussed.

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Acknowledgements

This manuscript is largely an output from projects funded by the Plant Science Research Programme (contracts R6951 and R7979) of the UK Department for International Development (DFID) for the benefit of developing countries. The views expressed are not necessarily those of DFID. KMD acknowledges funding from the Biotechnology and Biological Sciences Research Council (BBSRC) through a David Phillips Research Fellowship.

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Authors and Affiliations

  1. John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK

    X. Qi, T. S. Pittaway, S. Lindup, H. Liu, E. Waterman, F. K. Padi, J. Zhu, M. D. Gale & K. M. Devos

  2. ICRISAT, Patancheru, 502 324, Andhra Pradesh, India

    C. T. Hash

  3. Scottish Crop Research Institute, Invergowry, Dundee, DD2 5DA, UK

    H. Liu

  4. Savanna Agricultural Research Institute, P.O. Box 52, Nyankpala/Tamale, Northern Region, Ghana

    F. K. Padi

  5. Department of Crop and Soil Sciences and Department of Plant Biology, The University of Georgia, 3111 Miller Plant Sciences Building, Athens, GA, 30602-7271, USA

    K. M. Devos

Authors
  1. X. Qi
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  2. T. S. Pittaway
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  3. S. Lindup
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  4. H. Liu
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  5. E. Waterman
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  6. F. K. Padi
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  7. C. T. Hash
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  8. J. Zhu
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  9. M. D. Gale
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  10. K. M. Devos
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Correspondence to K. M. Devos.

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Communicated by P. Langridge

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Qi, X., Pittaway, T.S., Lindup, S. et al. An integrated genetic map and a new set of simple sequence repeat markers for pearl millet, Pennisetum glaucum. Theor Appl Genet 109, 1485–1493 (2004). https://doi.org/10.1007/s00122-004-1765-y

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  • DOI: https://doi.org/10.1007/s00122-004-1765-y

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