Theoretical and Applied Genetics

, Volume 109, Issue 5, pp 1070–1076 | Cite as

Genomic constitution and variation in five partial amphiploids of wheat–Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis

  • Fangpu HanEmail author
  • Bao Liu
  • George FedakEmail author
  • Zhaohui Liu
Original Paper


Genomic in situ hybridization (GISH) and multicolor GISH (mcGISH) methodology were used to establish the cytogenetic constitution of five partial amphiploid lines obtained from wheat × Thinopyrum intermedium hybridizations. Line Zhong 1, 2n=52, contained 14 chromosomes from each of the wheat genomes plus ten Th. intermedium chromosomes, with one pair of A-genome chromosomes having a Th. intermedium chromosomal segment translocated to the short arm. Line Zhong 2, 2n=54, had intact ABD wheat genome chromosomes plus 12 Th. intermedium chromosomes. The multicolor GISH results, using different fluorochrome labeled Th. intermedium and the various diploid wheat genomic DNAs as probes, indicated that both Zhong 1 and Zhong 2 contained one pair of Th. intermedium chromosomes with a significant homology to the wheat D genome. High-molecular-weight (HMW) glutenin and gliadin analysis revealed that Zhong 1 and Zhong 2 had identical banding patterns that contained all of the wheat bands and a specific HMW band from Th. intermedium. Zhong 1 and Zhong 2 had good HMW subunits for wheat breeding. Zhong 3 and Zhong 5, both 2n=56, possessed no gross chromosomal aberrations or translocations that were detectable at the GISH level. Zhong 4 also had a chromosome number of 2n=56 and contained the complete wheat ABD-genome chromosomes plus 14 Th. intermedium chromosomes, with one pair of Th. intermedium chromosomes being markedly smaller. Multicolor GISH results indicated that Zhong 4 also contained two pairs of reciprocally translocated chromosomes involving the A and D genomes. Zhong 3, Zhong 4 and Zhong 5 contained a specific gliadin band from Th. intermedium. Based on the above data, it was concluded that inter-genomic transfer of chromosomal segments and/or sequence introgression had occurred in these newly synthesized partial amphiploids despite their diploid-like meiotic behavior and disomic inheritance.


Wheat Chromosome Genomic Change Alien Chromosome Genomic Constitution Wheat Streak Mosaic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We are grateful to Mr. Zhang Yanbin and Ms. Gao Zhi for technical assistance in the seed storage protein analysis and for maintaining the plants over the years. This study was supported in part by the National Science Fund for Distinguished Young Scholars of China (no. 30225003).


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Eastern Cereal and Oilseed Research CentreAgriculture and Agri-Food CanadaOttawaCanada
  2. 2.Division of Biological SciencesUniversity of MissouriColumbiaUSA
  3. 3.School of Life SciencesNortheast Normal UniversityChangchunChina
  4. 4.Department of Plant PathologyNorth Darkata UniversityFargoUSA

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