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Pine genes regulated by the necrotrophic pathogen Fusarium circinatum

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Abstract

A targeted genomics approach was used to construct a cDNA array of potential pathogen-regulated genes for investigating host–pathogen interactions in pine trees (Pinus species). This array, containing a nonredundant set of 311 cDNAs, was assembled by combining smaller sets of cDNAs generated by differential display or suppression subtraction hybridization using a variety of pathogen treatments and elicitors. The array was probed to identify host genes regulated by Fusarium circinatum, a necrotrophic fungus that incites pitch canker disease on pine stems. A set of 29 cDNAs were induced during the disease state. Notably, cDNAs on the array that were derived from experiments with fusiform rust, incited by Cronartium quercuum f. sp. fusiforme (a biotrophic fungus) were unregulated by Fusarium. The results imply distinct genetic responses in pine to diseases incited by necrotrophs and biotrophs. This cDNA collection expands the genomics toolkit for understanding interactions between conifers and their microbial associates in forest ecosystems.

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Acknowledgements

This is journal article number R-10259 of the Florida Agricultural Experiment Station. The work was supported by the USDA Forest Service-Southern Research Station (Agreement no. SRS 00-CA-11330126-016), USDA-CSREES IFAFS (Grant no. 2001-52100-11315), and the Consortium for Plant Biotechnology (Agreement no. OR 22072-104) (to J.D.) and Georgia’s Traditional Industries Program in Pulp and Paper (to S.C.). We gratefully acknowledge T. Korhnak for technical assistance on differential display, C. Dervinis for critical assistance with plant maintenance and suggestions on the manuscript, and Basma El-Yacoubi for helpful discussions.

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Authors and Affiliations

  1. School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL 32611, USA

    Alison M. Morse, Katherine E. Smith & John M. Davis

  2. USDA Forest Service, Southern Institute of Forest Genetics, 23332 Highway 67, Saucier, MS 39574, USA

    C. Dana Nelson & Katherine E. Smith

  3. Daniel B. Warnell School of Forest Resources, University of Georgia, Athens, GA 30602, USA

    Sarah F. Covert & Angela G. Holliday

  4. Program in Plant Molecular and Cellular Biology, University of Florida, P.O. Box 110410, Gainesville, FL 32611, USA

    John M. Davis

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  1. Alison M. Morse
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  2. C. Dana Nelson
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  3. Sarah F. Covert
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  4. Angela G. Holliday
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  5. Katherine E. Smith
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  6. John M. Davis
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Correspondence to John M. Davis.

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Communicated by D.B. Neale

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Morse, A.M., Nelson, C.D., Covert, S.F. et al. Pine genes regulated by the necrotrophic pathogen Fusarium circinatum . Theor Appl Genet 109, 922–932 (2004). https://doi.org/10.1007/s00122-004-1719-4

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  • DOI: https://doi.org/10.1007/s00122-004-1719-4

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