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Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection

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Abstract

The distal segment of the long arm of the Thinopyrum intermedium chromosome 7Ai1 carries the barley yellow dwarf virus (BYDV) resistance gene Bdv2. This segment was transferred to the distal region of the long arm of wheat chromosome 7D in the Yw series of translocation lines by using the ph1b mutant to induce homoeologous pairing. To transfer Bdv2 to commercial varieties, we developed two resistance gene-analog polymorphism (RGAP) markers, Tgp-1350 and Tgp-2210, and one randomly amplified polymorphic DNA (RAPD) marker, OPD041300. The diagnostic fragments of the RGAP marker Tgp-1350 and the RAPD marker OPD041300 were cloned, sequenced and converted into sequence-characterized amplified region (SCAR) markers, named SC-gp1 and SC-D04, respectively. SC-gp1 and SC-D04 were validated based on available translocation lines and segregating F2 individuals. Our results indicated that the SCAR markers co-segregated with the BYDV resistance associated with Bdv2. Therefore, they can be used as a low-cost, high-throughput alternative to conventional phenotypic screening in wheat-breeding programs exploiting Bdv2. The marker-assisted selection for BYDV resistance was successfully performed in a wheat-breeding program.

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Acknowledgements

The study was supported by the national “Hi-Tech” project of China (2001AA222093). We are grateful to associate-professor Yan Liu for providing viruliferous aphids.

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Authors and Affiliations

  1. Key Laboratory of Crop Genetics and Breeding of Ministry of Agriculture, Institute of Crop Breeding and Cultivation, Chinese Academy of Agriculture Sciences, Beijing, 100081, China

    Zengyan Zhang, Jingsheng Xu, Qinjuan Xu & Zhiyong Xin

  2. CSIRO Plant Industry, GPO Box 1600, Canberra, 2601, ACT, Australia

    Philip Larkin

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  1. Zengyan Zhang
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  2. Jingsheng Xu
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  3. Qinjuan Xu
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  4. Philip Larkin
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  5. Zhiyong Xin
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Correspondence to Zhiyong Xin.

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Communicated by F. Salamini

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Zhang, Z., Xu, J., Xu, Q. et al. Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection. Theor Appl Genet 109, 433–439 (2004). https://doi.org/10.1007/s00122-004-1649-1

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  • DOI: https://doi.org/10.1007/s00122-004-1649-1

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