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Tetraploid races of Paspalum notatum show polysomic inheritance and preferential chromosome pairing around the apospory-controlling locus

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Abstract

The objective of this work was to determine the type of inheritance (disomic/polysomic) in tetraploid (2n=4x=40) Paspalum notatum and investigate the transmission pattern of the chromosome region associated with apospory. An F1 family segregating for the reproductive mode (aposporous vs non-aposporous) was generated by crossing a tetraploid sexual plant as female parent with an apomictic individual as pollen donor. Pollen mother cells from both parental plants were examined to ascertain chromosome-pairing behavior at meiosis. The high rate of quadrivalent chromosome associations indicated an autotetraploid origin of the species, although bivalent pairing and occasional univalents were detected. The observation of a lagging bivalent, a bridge of chromatin, or two aligned laggards in the aposporous parent suggested a chromosome inversion in this strain. Segregation ratios of AFLP markers and the proportion of linkages in repulsion versus coupling phase denoted tetrasomic inheritance, but markers displaying disomic ratios were also observed. Preferential chromosome pairing (disomic inheritance) in the chromosome segment related to apospory was detected. The possible relationship between a chromosome rearrangement and the inheritance of apospory is discussed.

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Acknowledgments

This study was financed by Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), projects nos. 6134 and 6367, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; and the International Foundation for Science (IFS), grant no. C/2870–2, Sweden. J. Stein and E.J. Martínez received fellowships from ANPCyT and CONICET, respectively. S.C. Pessino, C.L. Quarin, and J.P.A. Ortiz are research staff members of CONICET.

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Author notes

  1. J. P. A. Ortiz

    Present address: Instituto de Botánica del Nordeste (IBONE), Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste (UNNE), CC 209, 3400 , Corrientes, Argentina

Authors and Affiliations

  1. Laboratorio de Fisiología Vegetal, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario (UNR), CC14, 2125 , Zavalla, Pcia. de Santa Fe, Argentina

    J. Stein & J. P. A. Ortiz

  2. Instituto de Botánica del Nordeste (IBONE), Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste (UNNE), CC 209, 3400 , Corrientes, Argentina

    C. L. Quarin & E. J. Martínez

  3. Instituto de Biología Molecular y Celular de Rosario (IBR), Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Suipacha 531, 2000 , Rosario, Argentina

    S. C. Pessino

Authors
  1. J. Stein
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  2. C. L. Quarin
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  3. E. J. Martínez
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  4. S. C. Pessino
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  5. J. P. A. Ortiz
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Correspondence to J. P. A. Ortiz.

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Communicated by H.C. Becker

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Stein, J., Quarin, C.L., Martínez, E.J. et al. Tetraploid races of Paspalum notatum show polysomic inheritance and preferential chromosome pairing around the apospory-controlling locus. Theor Appl Genet 109, 186–191 (2004). https://doi.org/10.1007/s00122-004-1614-z

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  • DOI: https://doi.org/10.1007/s00122-004-1614-z

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