Abstract
Breeding for resistant cultivars is the only way to prevent high yield loss in barley caused by the soil-borne barley mild mosaic virus (BaMMV) complex. We have characterized the BaMMV resistance of barley cv. Chikurin Ibaraki 1. Doubled haploid lines were obtained from the F1 between the susceptible six-rowed winter barley cultivar, Plaisant, and Chikurin Ibaraki 1. Each line was tested for reaction to BaMMV by mechanical inoculation followed by DAS-ELISA. Of 44 microsatellites that covered the genome, 22 polymorphic markers were tested on one susceptible and one resistant bulk, each comprising 30 lines. Differential markers and additional microsatellite markers in the same region were then tested on the whole population. A bootstrap analysis was used to compute confidence intervals of distances and to test the orders of the resistance gene and the closest markers. A segregation of 84 resistant/98 susceptible lines fitted a 1:1 ratio (χ2=1.08, P=0.30), which corresponds to a single gene in this DH lines population. The resistance gene was flanked by two markers near the centromeric region of chromosome 6HS—Bmag0173, at 0.6±1.2 cM, and EBmac0874, at 5.8 ± 3.4 cM. We propose to name this new resistance gene rym15. This resistance gene and associated markers will increase the possibilities to breed efficiently for new cultivars resistant to the barley mosaic disease.
Similar content being viewed by others
References
Bauer E, Weyen J, Schiemman A, Graner A, Ordon F (1997) Molecular mapping of novel resistance genes against barley mild mosaic virus (BaMMV). Theor Appl Genet 95:1263–1269
Chen J, Adams MJ, Zhu FT, Wang ZQ, Chen J, Huang SZ, Zhang ZC (1996) Responses of foreign barley cultivars to barley yellow mosaic viruses at different sites in China. Plant Pathol 45:1117–1125
Devaux P, Zivy M (1994) Protein markers for anther culturability in barley. Theor Appl Genet 88:701–706
Devaux P, Kilian A, Kleinhofs A (1995) Comparative mapping of the barley genome with male and female recombination-derived, doubled haploid populations. Mol Gen Genet 249:600–608
Friedt W (1983) Mechanical transmission of soil-borne barley yellow mosaic virus. Phytopathol Z 106:16–22
Götz R, Friedt W (1993) Resistance to the barley yellow mosaic virus complex—differential genotypic reactions and genetics of BaMMV-resistance of barley (Hordeum vulgare L). Plant Breed 111:125–131
Graner A, Jahoor A, Schondelmaier J, Siedler H, Pillen K, Fishbeck G, Wenzel G, Hermann RG (1991) Construction of an RFLP map of barley. Theor Appl Genet 83:250–256
Graner A, Streng S, Kellermann A, Schiemann A, Bauer E, Waugh R, Pellio B, Ordon F (1999) Molecular mapping and genetic fine-structure of the rym5 locus encoding resistance to different strains of the barley yellow mosaic complex. Theor Appl Genet 98:285–290
Hariri D, Meyer M, Le Gouis J, Bahrman N, Fouchard M, Forget C, Andre A (2000) Characterisation of BaYMV and BaMMV pathotypes in France. Eur J Plant Pathol 106:365–372
Hariri D, Meyer M, Prud’homme H (2003) Characterization of a new barley mild mosaic virus pathotype in France. Eur J Plant Pathol 109:921–928
Huth W (1989) Ein weiterer Stamm des Barley yellow mosaic virus (BaYMV) gefunden. Nachrichtenbl Dtsch Pflanzenschutzdienstes 41:6–7
Huth W, Adams MJ (1990) Barley yellow mosaic virus (BaYMV) and BAYMV-M: two different viruses. Intervirology 31:38–42
Huth W, Leseman D (1978) Eine für die Bundesrepublik neue Virose an Wintergerste. Nachrichtenbl Dtsch Pflanzenschutzdienstes 30:184–185
Iida Y, Watanabe K, Toshima I, Ogawa K (1992) Reaction of barley (Hordeum vulgare L.) cultivars and lines to barley yellow mosaic virus strains. Jpn J Breed 42:863–877
Ikata S, Kawai I (1940) Studies on wheat yellow mosaic disease. Noji Kairo Shiryo 154:1–123
Kashiwazaki S, Hayano Y, Minobe Y, Omura T, Hibino H, Tsuchizaki T (1989) Nucleotide sequence of the capsid protein gene of barley yellow mosaic virus. J Gen Virol 70:3015–3023
Konishi T, Ban Y, Iida Y, Yoshimi R (1997) Genetic analysis of disease resistance to all strains of BAYMV in a Chinese barley landrace, Mokusekko 3. Theor Appl Genet 94:871–877
Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–178
Le Gouis J, Ordon F, de Froidmont D, Béghin D, Jestin L, Joseph J-L, Froidmont F (1999) Agronomic comparison of two sets of SSD lines differing for the ym4 resistance gene against barley mosaic viruses. Agronomie 19:125–131
Le Gouis J, Hariri D, Bahrman N, Jestin L (2000) Resistance of old French barley cultivars to barley mosaic viruses. Z Planzenkr Pflanzenschutz 107:33–438
Lee K-J, Kashiwazaki S, Hibi T, So I-Y (1996) Properties and capsid protein gene sequence of a Korean isolate of barley mild mosaic virus. Ann Phytopathol Soc Jpn 62:397–401
Li JZ, Sjakste TG, Röder MS, Ganal MW (2003) Development and genetic mapping of 127 new microsatellite markers in barley. Theor Appl Genet 107:1021–1027
Liu BH (1998) Statistical genomics: linkage, mapping and QTL analysis. CRC press, Boca Raton, New York
Liu ZW, Biyashev RM, Saghai Maroof MA (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theor Appl Genet 93:869–876
Manninen OM (2000) Associations between anther-culture response and molecular markers on chromosomes 2H, 3H and 4H of barley (Hordeum vulgare L.) Theor Appl Genet 100:57–62
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Nomura K, Kashiwazaki S, Hibino H, Inoue T, Nakata E, Tsuzaki E, Okuyama S (1996) Biological and serological properties of strains of barley mild mosaic virus. J Phytopathol 144:103–107
Okada Y, Kashiwazaki S, Kanatani R, Arai S, Ito K (2003) Effects of barley yellow mosaic disease resistant gene rym1 of the infection strains of barley yellow mosaic virus and barley mild mosaic virus. Theor Appl Genet 106:181–189
Ordon F, Friedt W (1993) Mode of inheritance and genetic diversity of BaMMV resistance of exotic barley germplasms carrying genes different from ‘ym4’. Theor Appl Genet 86:229–233
Ordon F, Schiemann A, Friedt W (1997) Assessment of the genetic relatedness of barley accessions (Hordeum vulgare L.) resistant to soil-borne mosaic inducing viruses (BaMMV, BaYMV, BaYMV-2) using RAPDs. Theor Appl Genet 94:325–330
Ramsay L, Macaulay M, degli Ivanissevich S, MacLean K, Cardle L, Fuller J, Edwards KJ, Tuvesson S, Morgante M, Massari A, Maestri E, Marmiroli N, Sjakste T, Ganal M, Powell W, Waugh R (2000) A simple sequence repeat-based linkage map of barley. Genetics 156:1997–2005
Ruge B, Linz A, Pickering G, Greif P, Wehling P (2003) Mapping of Rym14 Hb, a gene introgressed from Hordeum bulbosum and conferring resistance to BaMMV and BaYMV in barley. Theor Appl Genet 107:965–971
Toyama A, Kusaba T (1970) Transmission of soil-borne barley yellow mosaic virus .2.Polymyxa graminis Led as vector. Ann Phytopathol Soc Jpn 36:223–229
Ukai Y, Yamashita A (1980) Induced mutation for resistance to barley yellow mosaic virus. Jpn J Breed 30:125–130
Werner K, Friedt W, Ordon F (2000a) Strategies for pyramiding resistance genes against the barley yellow mosaic complex based on molecular markers and DH-lines. In: Logue S (ed) 8th Int Barley Genet Symp, vol 2. Department of Plant Science, Adelaide University Press, Adelaide, Australia, pp 200–202
Werner K, Pellio B, Ordon F, Friedt W (2000b) Development of an STS marker and SSRs markers suitable for marker-assisted selection for the BaMMV resistance gene rym9 in barley. Plant Breed 119:517–519
Werner K, Fried W, Laubach E, Waugh R, Ordon F (2003a) Dissection of resistance to soil-borne yellow-mosaic-inducing viruses of barley (BaMMV, BaYMV, BaYMV-2) in a complex breeders cross by SSRs and simultaneous mapping of BaYMV/BaYMV-2 resistance of Chikurin Ibaraki 1. Theor Appl Genet 106:1425–1432
Werner K, Rönicke S, Le Gouis J, Friedt W, Ordon F (2003b) Mapping of a new BaMMV-resistance gene derived from the variety Taihoku A. J Plant Dis Protect 110: 304–311
Zivy M, Devaux P, Blaisonneau J, Jean R, Thiellement H (1992) Segregation distortion and linkage studies in microspore-derived double haploid lines of Hordeum vulgare L. Theor Appl Genet 83:919–924
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by P. Langridge
Rights and permissions
About this article
Cite this article
Le Gouis, J., Devaux, P., Werner, K. et al. rym15 from the Japanese cultivar Chikurin Ibaraki 1 is a new barley mild mosaic virus (BaMMV) resistance gene mapped on chromosome 6H. Theor Appl Genet 108, 1521–1525 (2004). https://doi.org/10.1007/s00122-003-1571-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00122-003-1571-y