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BAC-derived diagnostic markers for sex determination in asparagus

Abstract

A HindIII BAC (bacterial artificial chromosome) library of asparagus (Asparagus officinalis L.) was established from a single male plant homozygous for the male flowering gene (MM). The library represents approximately 5.5 haploid genome equivalents with an average insert size of 82 kb. A subset of the library (2.6 haploid genome equivalents) was arranged into DNA pools. Using nine sex-linked amplified fragment length polymorphism (AFLP) and two sequence-tagged site (STS) markers, 13 different BAC clones were identified from this part of the library. The BACs were arranged into a first-generation physical map around the sex locus. Four PCR-derived markers were developed from the BAC ends, one of which could be scored in a co-dominant way. Using a mapping population of 802 plants we mapped the BAC-derived markers to the same position close to the M gene as the corresponding AFLP and STS markers. The markers are useful for further chromosome walking studies and as diagnostic markers for selecting male plants homozygous for the M gene.

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Acknowledgements

The authors are indebted to Dr. B. Weisshaar, Max Planck Institute for Plant Breeding Research (ADIS), Cologne, for support in automated colony picking. We thank Monika Bruisch for her excellent technical assistance. This project was supported by the Deutsche Forschungsgemeinschaft (DFG) (grant no. Ju 205/2-5) and the German Academic Exchange Service (DAAD). This paper is dedicated to Prof. Dr. Dr. h.c. Günter Wricke on the occasion of his 75th birthday.

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Correspondence to C. Jung.

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Communicated by G. Wenzel

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Jamsari, A., Nitz, I., Reamon-Büttner, S.M. et al. BAC-derived diagnostic markers for sex determination in asparagus. Theor Appl Genet 108, 1140–1146 (2004). https://doi.org/10.1007/s00122-003-1529-0

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Keywords

  • Amplify Fragment Length Polymorphism
  • Amplify Fragment Length Polymorphism Marker
  • Cleave Amplify Polymorphic Sequence
  • Female Plant
  • Male Plant