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A novel locus for clubroot resistance in Brassica rapa and its linkage markers

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Abstract

An inbred turnip (Brassica rapa syn. campestris) line, N-WMR-3, which carries the trait of clubroot resistance (CR) from a European turnip, Milan White, was crossed with a clubroot-susceptible doubled haploid line, A9709. A segregating F3 population was obtained by single-seed descent of F2 plants and used for a genetic analysis. Segregation of CR in the F3 population suggested that CR is controlled by a major gene. Two RAPD markers, OPC11-1 and OPC11-2, were obtained as candidates of linkage markers by bulked segregant analysis. These were converted to sequence-tagged site markers, by cloning and sequencing of the polymorphic bands, and named OPC11-1S and OPC11-2S, respectively. The specific primer pairs for OPC11-1S amplified a clear dominant band, while the primer pairs for OPC11-2S resulted in co-dominant bands. Frequency distributions and statistical analyses indicate the presence of a major dominant CR gene linked to these two markers. The present marker for CR was independent of the previously found CR loci, Crr1 andCrr2. Genotypic distribution and statistical analyses did not show any evidence of CR alleles on Crr1 andCrr2 loci in N-WMR-3. The present study clearly demonstrates that B. rapa has at least three CR loci. Therefore, the new CR locus was named Crr3. The present locus may be useful in breeding CR Chinese cabbage cultivars to overcome the decay of present CR cultivars.

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Acknowledgements

This investigation was partly supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 1340006) to M.H.

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Authors and Affiliations

  1. Faculty of Agriculture, Kyoto Prefectural University, Kita-Inayazuma, Seika, Kyoto 619-0244, Japan

    M. Hirai, T. Harada & N. Kubo

  2. Nagano Vegetable and Ornamental Crops Experiment Station, Matsushiro, Nagano 381-1211, Japan

    M. Tsukada

  3. National Institute of Vegetables and Tea Science (NIVTS), Ano, Mie 514-2392, Japan

    K. Suwabe & S. Matsumoto

Authors
  1. M. Hirai
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  2. T. Harada
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  3. N. Kubo
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  4. M. Tsukada
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  5. K. Suwabe
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  6. S. Matsumoto
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Correspondence to M. Hirai.

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Communicated by C. Möllers

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Hirai, M., Harada, T., Kubo, N. et al. A novel locus for clubroot resistance in Brassica rapa and its linkage markers. Theor Appl Genet 108, 639–643 (2004). https://doi.org/10.1007/s00122-003-1475-x

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  • DOI: https://doi.org/10.1007/s00122-003-1475-x

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