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Delimitation of the chromosomal region for a quantitative trait locus, qUVR-10, conferring resistance to ultraviolet-B radiation in rice (Oryza sativa L.)

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Abstract

Wide variation in ultraviolet-B (UVB) resistance is observed among rice varieties. In a previous study, three quantitative trait loci (QTLs) controlling UVB resistance were detected by QTL analysis, using backcross inbred lines (BILs) derived from a cross between a japonica cultivar, ‘Nipponbare’, and an indica cultivar, ‘Kasalath’. Among them, qUVR-10, a QTL for UVB resistance on chromosome 10, showed the largest effect. Plants homozygous for the Nipponbare allele at qUVR-10 were resistant to UVB, unlike those homozygous for the Kasalath allele. To determine more precisely the chromosomal location of qUVR-10, we performed a linkage mapping of qUVR-10 as a single Mendelian factor using advanced backcross progeny. Advanced progeny testing of F4 families enabled us to determine the genotype classes of the qUVR-10 locus with high reliability. As a result, qUVR-10 was mapped between RFLP markers C60755S and C1757S, and co-segregated with C913A. In addition, a sequence showing high similarity to the Arabidopsis cyclobutane pyrimidine dimer (CPD) photolyase gene, which has been found to be involved in sensitivity to UV radiation in Arabidopsis and rice, was mapped in the candidate genomic region of qUVR-10. This result suggests that the CPD photolyase gene is a positional candidate for qUVR-10.

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Acknowledgements

We thank Mr. Shoichi Musashi, Mrs. Masako Takahashi and Mrs. Momoe Iizumi for their technical assistance. This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project MP-1121) and by a Grant-in Aid (No. 10556075 and No. 15201010) for Scientific Research from the Ministry of Education, Culture and Science of Japan.

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Correspondence to T. Ueda.

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Communicated by D.J. Mackill

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Ueda, T., Sato, T., Numa, H. et al. Delimitation of the chromosomal region for a quantitative trait locus, qUVR-10, conferring resistance to ultraviolet-B radiation in rice (Oryza sativa L.). Theor Appl Genet 108, 385–391 (2004). https://doi.org/10.1007/s00122-003-1460-4

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