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Quantitative trait locus analysis of fungal disease resistance factors on a molecular map of grapevine

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Abstract

A full-sibling F1 population comprising 153 individuals from the cross of ‘Regent’ × ‘Lemberger’ was employed to construct a genetic map based on 429 molecular markers. The newly-bred red grapevine variety ‘Regent’ has multiple field-resistance to fungal diseases inherited as polygenic traits, while ‘Lemberger’ is a traditional fungus-susceptible cultivar. The progeny segregate quantitatively for resistances to Plasmopara viticola and Uncinula necator, fungal pathogens that threaten viticulture in temperate areas. A double pseudo-testcross strategy was employed to construct the two parental maps under high statistical stringency for linkage to obtain a robust marker frame for subsequent quantitative trait locus (QTL) analysis. In total, 185 amplified fragment length polymorphism, 137 random amplified polymorphic DNA, 85 single sequence repeat and 22 sequence characterized amplified region or cleaved amplified polymorphic sequence markers were mapped. The maps were aligned by co-dominant or doubly heterozygous dominant anchor markers. Twelve pairs of homologous linkage groups could be integrated into consensus linkage groups. Resistance phenotypes and segregating characteristics were scored as quantitative traits in three or four growing seasons. Interval mapping reproducibly localized genetic factors that correlated with fungal disease resistances to specific regions on three linkage groups of the maternal ‘Regent’ map. A QTL for resistance to Uncinula necator was identified on linkage group 16, and QTLs for endurance to Plasmopara viticola on linkage groups 9 and 10 of ‘Regent’. Additional QTLs for the onset of berry ripening (“veraison”), berry size and axillary shoot growth were identified. Berry color segregated as a simple trait in this cross of two red varieties and was mapped as a morphological marker. Six markers derived from functional genes could be localized. This dissection of polygenic fungus disease resistance in grapevine allows the development of marker-assisted selection for breeding, the characterization of genetic resources and the isolation of the corresponding genes.

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Acknowledgements

We would like to thank Bruce Reisch, Herta Steinkellner and Ferdinand Regner for communicating primer sequences prior to publication. Carole Meredith and Stella Grando provided information on grapevine genetic maps prior to publication. Sabine Wiedemann-Merdinoglu (INRA Colmar) participated in Plasmopara viticola resistance phenotyping of the plants with special expertise. AGROGENE SA (Moissy Cramayel, France) organized the SSR development in a joint effort of more than 20 participating research groups. This work was supported by the Deutsche Forschungsgemeinschaft (DFG, Zy11-4). Fellowships have been provided to Ilkhom Salakhutdinov (Deutscher Akademischer Austauschdienst, DAAD; Freunde und Förderer des Instituts für Rebenzüchtung Geilweilerhof) and Murat Akkurt (student fellowship from the University of Ankara) to facilitate their contribution to this work.

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Correspondence to E. M. Zyprian.

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Communicated by H.C. Becker

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Fischer, B.M., Salakhutdinov, I., Akkurt, M. et al. Quantitative trait locus analysis of fungal disease resistance factors on a molecular map of grapevine. Theor Appl Genet 108, 501–515 (2004). https://doi.org/10.1007/s00122-003-1445-3

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