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First RFLP linkage map of red clover (Trifolium pratense L.) based on cDNA probes and its transferability to other red clover germplasm

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Abstract

We constructed a genetic linkage map of red clover (Trifolium pratense L., 2n=2x=14) using RFLP markers from cDNA probes of a backcrossed mapping population, and investigated the transferability of the markers to other red clover germplasm. The map contains 157 RFLP markers and one morphological marker on seven linkage groups. The total map distance was 535.7 cM and the average distance between two markers was 3.4 cM. All of the cDNA probes of the map were hybridized to the fragments of genomic DNA from 12 plants derived from three varieties, and 87% of the cDNA probes detected polymorphic bands that corresponded to those of mapping parents. This result indicated that RFLP markers on the present map were transferable to the genome analysis of other red clover germplasm. This is the first report to construct a linkage map of Trifolium species; it should provide fundamental and useful genetic information relevant to the breeding of red clover and genus Trifolium.

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Acknowledgements

The authors thank Toyoko Shibata and Yoshimi Saitoh for their technical assistance. This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project DM-2102 and Low Temperature Stress Project). This work is part of an ongoing joint research project between the National Agricultural Research Center for the Hokkaido Region in Japan and the All-Russian Williams Fodder Crop Research Institute in Russia.

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Authors and Affiliations

  1. National Agricultural Research Center for Hokkaido Region, Hitsujigaoka 1, Toyohira, Sapporo, 062-8555, Hokkaido, Japan

    S. Isobe & M. Gau

  2. All-Russian Williams Fodder Crop Research Institute, 141740 Lugovaya, Moscow region, Russia

    I. Klimenko, S. Ivashuta & N. N. Kozlov

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  1. S. Isobe
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  2. I. Klimenko
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  3. S. Ivashuta
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  4. M. Gau
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  5. N. N. Kozlov
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Correspondence to S. Isobe.

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Communicated by H.C. Becker

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Isobe, S., Klimenko, I., Ivashuta, S. et al. First RFLP linkage map of red clover (Trifolium pratense L.) based on cDNA probes and its transferability to other red clover germplasm. Theor Appl Genet 108, 105–112 (2003). https://doi.org/10.1007/s00122-003-1412-z

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  • DOI: https://doi.org/10.1007/s00122-003-1412-z

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