Skip to main content
SpringerLink
Log in

Genetic analysis, molecular tagging and mapping of the thermo-sensitive genic male-sterile gene (wtms1) in wheat

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

A thermo-sensitive genic male-sterile (TGMS) wheat line (Triticum aestivum L.) BNY-S was obtained from the spontaneous mutant of BNY-F. Its fertility was decided by the temperature during the differentiation stage of the spikelets. BNY-S was completely sterile when the temperature was lower than 10°C during the differentiation stage of the spikelets, but fertile when the temperature was higher than 10°C. Genetic analysis indicated that the sterility of BNY-S was controlled by a single recessive gene, which was named as wtms1. An F2 population, consisting of 3,000 individuals from the cross between BNY-S and Lankao 52-24, was used for genetic analysis and statistical analysis of the TGMS and, out of them, 158 sterile and 93 fertile extremes were present for molecular tagging and mapping of the wtms1 gene. SSR (simple sequence repeat) and AFLP (amplified fragment length polymorphism) techniques combined with BSA (bulked segregant analysis) were used to screen markers linked to the target gene. As a result, wtms1 was preliminarily mapped on chromosome 2B according to SSR analysis. In AFLP analysis, 14 polymorphic AFLP loci were identified with a linkage relation to the wtms1 gene. Then linkage analysis using the F2 population showed that three of them, E: AAG/M: CTA163, E: AGG/M: CTC220 and E: ACA/M: CTA160, were linked to the wtms1 gene relatively close to a genetic distance of 6.9 cM, 6.9 cM and 13.9 cM, respectively. Finally, the wtms1 gene was mapped between the SSR marker Xgwm 374 and the AFLP marker E: AAG/M: CTA163 with the distance of 4.8 cM and 6.9 cM, respectively. A partial linkage map was constructed according the SSR and AFLP data.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1A,B
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Cao SH, Liu DC, Liu LK, Guo XL, Zhang AM (2002) Preliminary mapping of genes concerning photoperiod-temperature sensitive male sterility in wheat (Triticum aestivum L.). In: The Abstract Book of the 3rd National Plant Genome Meeting in China, P23, Beijing, China

  • Cao SH, Liu DC, Liu LK, Guo XL, Zhang AM (2003) Differential expression of genes related to photoperiod-temperature sensitive male sterility in wheat, revealed by mRNA differential display using the G-box family primer. Acta Genet Sinica 30:56–61

    Google Scholar 

  • Chantret N, Sourdille P, Röder M, Tavaud M, Bernard M, Doussinault G (2000) Location and mapping of the powdery mildew resistance gene MlRE and detection of a resistance QTL by bulked segregant analysis (BSA) with microsatellites in wheat. Theor Appl Genet 100:1217–1224

    CAS  Google Scholar 

  • Dong NV, Subudhi PK, Luong PN, Quang VD, Quy TD, Zheng HG, Wang B, Nguyen HT (2000) Molecular mapping of a rice gene conditioning thermosensitive genic male sterility using AFLP, RFLP and SSR techniques. Theor Appl Genet 100:727–734

    CAS  Google Scholar 

  • Hoisington D, Khairallah M, González-de-León D (1994) Laboratory protocols: CIMMYT applied molecular genetics laboratory. CIMMYT, Mexico, DF

    Google Scholar 

  • Jia JH, Zhang DS, Li CY, Qu XP, Wang SW, Chamarerk V, Nguyen HT, Wang B (2001) Molecular mapping of the reverse thermo-sensitive genic male-sterile gene (rtms1) in rice. Theor Appl Genet 103:607–612

    CAS  Google Scholar 

  • Khush G.S (1999) Green revolution: preparing of the 21st century. Genome 42:646–655

    CAS  PubMed  Google Scholar 

  • Koh HJ, Son YH, Heu MH, Lee HS, McCouch SR (1999) Molecular mapping of a new genic male sterility gene causing chalky endosperm in rice (Oryza sativa L.). Euphytica 106:57– 62

    Article  CAS  Google Scholar 

  • Kosambi DD (1944) The estimation of the map from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Etoh T (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Lima MLA, Garcia AAF, Oliveira KM, Matsuoka S, Arizono H, de Souza Jr CL, de Souza AP (2002) Analysis of genetic similarity detected by AFLP and the coefficient of parentage among genotypes of sugar cane (Saccharum spp.). Theor Appl Genet 104:30–38

    CAS  Google Scholar 

  • Michelmore RW, Paran I, Kesseli V (1991) Identification of markers linked to a disease resistance gene by bulked segregant analysis: a rapid method to detect markers in a specific genomic region by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    PubMed  Google Scholar 

  • Plaschke J, Börner A, Wendehake K, Ganal MW, Röder MS (1996) The use of wheat aneuploids for the chromosomal assignment of microsatellite loci. Euphytica 89:33–40

    CAS  Google Scholar 

  • Röder MS, Plaschke J, Konig SU, Börner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246:327–333

    PubMed  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Subudhi PK, Bortatati RP, Virmani SS (1997) Molecular mapping of a thermo- sensitive genic male sterility gene in rice using bulked sergeant analysis. Genome 40:188–194

    CAS  Google Scholar 

  • Timothy A H, John TC, Linda M N, Harker R H (2002) Identification and mapping of polymorphic SSR markers from expressed gene sequences of barley and wheat. Mol Breed 9:63–71

    CAS  Google Scholar 

  • Wang B, Xu WW, Wang JZ, Wu W, Zheng HG, Yang ZY, Huang N, Ray JD, Nguyen HT (1995) Tagging and mapping the thermo-sensitive genic male-sterile gene in rice with molecular markers. Theor Appl Genet 91:1111–1114

    CAS  Google Scholar 

  • Wang T, Zhang ZS, Ao DH, Yu MQ (1998) Studies and application of thermo-photoperiod sensitive male sterility wheat C49S. In: Proc 9th Int Wheat Genetics Symp. University of Saskachewan 3:78–80

    Google Scholar 

  • Xu SB, Tao YF, Yang ZQ, Chu JY (2002) A simple and rapid methods used for silver staining and gel preservation. Hereditas 24:335–336

    Google Scholar 

  • Yamagushi Y, Ikeda R, Hirasawa H, Minami M, Ujihara P (1997) Linkage analysis of the thermo-sensitive genic male sterility gene tms2 in rice (Oryza sativa L.). Breed Sci 47:371–377

    Google Scholar 

  • Zhang AM, Huang TC (1998) Progress of hybrid wheat breeding in China. In: Zhang AM, Huang TC (eds) Proc 1st Int Workshop on Hybrid Wheat. China Angricultural University Press. Beijing, pp 9–14

  • Zhang QF, Shen BZ, Dai XK, Mei MH, Saghai Maroof MA, Li ZB (1994) Using the bulked extreme and recessive class to map genes for photoperiod sterility in rice. Proc Natl Acad Sci USA 91:8675–8679

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This study was partially supported by The NSF Project (30170490) of China and the State Key Basic Research and Developmental Plan of China (2001CB108802).

Author information

Authors and Affiliations

  1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China

    Q. H. Xing, C. J. Zhou, C. Y. Liang, D. E. Yang, D. M. Jin & B. Wang

  2. Henan Vocational-Technical Teachers' College, 453003, Xinxiang, China

    Z. G. Ru & X. Xue

Authors
  1. Q. H. Xing
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Z. G. Ru
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. J. Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. X. Xue
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. Y. Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D. E. Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. D. M. Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. B. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to B. Wang.

Additional information

Communicated by H.F. Linskens

Q.H. Xing and Z.G. Ru contributed equally to this manuscript

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xing, Q.H., Ru, Z.G., Zhou, C.J. et al. Genetic analysis, molecular tagging and mapping of the thermo-sensitive genic male-sterile gene (wtms1) in wheat. Theor Appl Genet 107, 1500–1504 (2003). https://doi.org/10.1007/s00122-003-1385-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-003-1385-y

Keywords

Search

Navigation