Skip to main content

Oryzalin-induced chromosome doubling in Rosa and its effect on plant morphology and pollen viability

Theoretical and Applied Genetics volume 107, pages 1195–1200 (2003)Cite this article

Abstract

Shoot tips of the diploid rose Thérèse Bugnet were treated in vitro to oryzalin at concentrations of 5 and 15 μM. Tetraploid shoots were obtained in highest frequencies (40%) after exposure to 5 μM oryzalin for 14 days. Thin (1 mm) nodal sections were treated with 5 μM oryzalin and the highest frequency of tetraploids (66%) was obtained after exposure for only 1 day. The shorter exposure times required to induce chromosome doubling in thin nodal sections is attributed to the more efficient delivery of oryzalin to the meristem. Tetraploids were obtained from four diploid roses and hexaploids from two triploid roses. Chromosome doubling was accompanied by increases in thickness and a darker green colouration of the leaves and, in all diploid to tetraploid and one triploid to hexaploid conversion, the breadth/length ratio of leaflets was significantly increased. Internodes were longer in tetraploids than diploids but significantly shorter in hexaploids than triploids. The number of petals per flower in the tetraploid form of Thérèse Bugnet was double that of the diploid. Significant increases in pollen viability accompanied chromosome doubling of all four diploids and one of the two triploids.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4A–E.
Fig. 5.
Fig. 6.
Fig. 7.

References

  • Basye R (1992) The future of the rose. Am Rose Annu 31:62–63

    Google Scholar 

  • Cairns T (ed) (2000) Modern roses XI. Academic Press, London

  • Darlington CD, Wylie AP (1955) Chromosome atlas of flowering plants, 2nd edn Allen and Unwin, London

  • Dubois LAM, de Vries DP (1996) The direct regeneration of adventitious buds on leaf explants of glasshouse-grown cut rose cultivars. Acta Hortic 424:327–329

    Google Scholar 

  • Fagerlind F (1958) Hip and seed formation in newly formed Rosa polyploids. Acta Hortic Berg 17:229–256

    Google Scholar 

  • Horan I, Walker S, Roberts AV, Mottley J, Simpkins I (1995) Micropropagation of roses: the benefits of pruned mother-plantlets at Stage II and a greenhouse environment at Stage III. J Hortic Sci 70:799–806

    Google Scholar 

  • Hugdahl JD, Morejohn LC (1993) Rapid and reversible high-affinity binding of the dinitroaniline herbicide oryzalin to tubulin from Zea mays L. Plant Physiol 102:725–740

    CAS  PubMed  Google Scholar 

  • Leitch IJ, Bennett MD, (1997) Polyploidy in angiosperms. Trends Plant Sci 2:470–476

    Article  Google Scholar 

  • Luckett DJ (1989) Colchicine mutagenesis is associated with substantial heritable variation in cotton. Euphytica 42:177–182

    CAS  Google Scholar 

  • Ma Y, Byrne DH, Chen J (1997) Amphidiploid induction from diploid rose interspecific hybrids. Hortscience 32:292–295

    Google Scholar 

  • Morejohn LC, Bureau TE, Molè-Bajer J, Bajer AS, Fosket DE (1987) Oryzalin, a dinitroaniline herbicide, binds to plant tubulin and inhibits microtubule polymerization in vitro. Planta 172:252–264

    CAS  Google Scholar 

  • Roberts AV, Lloyd D, Short KC (1990) In vitro procedures for the induction of tetraploidy in a diploid rose. Euphytica 49:33–38

    Google Scholar 

  • Semeniuk P, Arisumi T (1968) Colchicine-induced tetraploid and cytochimeral roses. Bot Gaz 129:190–193

    Article  Google Scholar 

  • Ueda Y (1994) Systematic studies in the genus Rosa. Techn Bull Fac Hortic, Chiba University, Japan 48:241–328

    Google Scholar 

  • Yokoya K, Roberts AV, Lewis R, Mottley J, Brandham PE (2000) Nuclear DNA amounts in roses. Ann Bot 85:557–561

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors acknowledge financial support from the Department of the Environment, Food and Rural Affairs, UK and David Austin Roses Ltd and thank Dr. Sara Walker for her help.

Author information

Author notes

  1. V. Sarasan

    Present address: Micropropagation Unit, Royal Botanic Gardens, Kew, Surrey, TW9 3AB, UK

  2. K. Yokoya

    Present address: Sir Alexander Flemming Building, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK

  3. J. Wentworth

    Present address: Royal Commission on Environmental Pollution, The Sanctuary, Westminster, London, SW1P 3JS, UK

  4. V. K. Sieber

    Present address: Medical Sciences Division, Department of Experimental Psychology, University of Oxford, Oxford, OX1 3UD, UK

Authors and Affiliations

  1. School of Health and Bioscience, University of East London, Romford Road, London, E15 4LZ, UK

    M. J. Kermani, V. Sarasan, A. V. Roberts, K. Yokoya, J. Wentworth & V. K. Sieber

Authors
  1. M. J. Kermani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. Sarasan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. V. Roberts
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Yokoya
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Wentworth
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. V. K. Sieber
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. V. Roberts.

Additional information

Communicated by H. Nybom

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kermani, M.J., Sarasan, V., Roberts, A.V. et al. Oryzalin-induced chromosome doubling in Rosa and its effect on plant morphology and pollen viability. Theor Appl Genet 107, 1195–1200 (2003). https://doi.org/10.1007/s00122-003-1374-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-003-1374-1

Keywords

  • Colchicine
  • Root Meristem
  • Pollen Viability
  • Chromosome Doubling
  • Oryzalin