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An AP2/EREBP-type transcription-factor gene from rice is cold-inducible and encodes a nuclear-localized protein

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Abstract

We cloned an AP2/EREBP gene by dot blotting and named it OsDREBL. Analysis of its deduced amino-acid sequence indicated that this protein had a potential nuclear-localization signal, a possible acidic-activation domain and an AP2 DNA binding domain. Northern analysis showed that the transcripts of OsDREBL accumulated rapidly (within 30 min) in response to low temperature, but not in response to ABA, NaCl and dehydration treatments. Southern analysis indicated the presence of a single-copy of the OsDREBL gene in the Oryza sativa genome. Our research also demonstrated that OsDREBL was localized to the nucleus but did not bind effectively to the C-repeat/dehydration responsive element (CRT/DRE). These results suggested that OsDREBL may function as a transcription factor in the cold-stress response, independent of the DREB signal-transduction pathway.

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Acknowledgements

This work was supported by the 863 project (2001 AAA222131, 2002AAA2Z1001 and 2001AA212121) and the 973 project (G19990117003).

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Institute of Genetics and Development Biology, The Chinese Academy of Sciences, Beijing 100101, China

    Jian-Quan Chen, Yi Dong, Yu-Jun Wang, Jin-Song Zhang & Shou-Yi Chen

  2. Department of Biological Sciences and Biotechnology, Tsinghua University, Tsinghua Yuan, Haidian, Beijing 100084, China

    Qiang Liu

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  1. Jian-Quan Chen
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  2. Yi Dong
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  3. Yu-Jun Wang
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  4. Qiang Liu
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  5. Jin-Song Zhang
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Correspondence to Jin-Song Zhang or Shou-Yi Chen.

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Communicated by H.F. Linskens

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Chen, JQ., Dong, Y., Wang, YJ. et al. An AP2/EREBP-type transcription-factor gene from rice is cold-inducible and encodes a nuclear-localized protein. Theor Appl Genet 107, 972–979 (2003). https://doi.org/10.1007/s00122-003-1346-5

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