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Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L.

Theoretical and Applied Genetics volume 107, pages 997–1002 (2003)Cite this article

Abstract

In an analysis of 114 F2 individuals from a cross between clubroot-resistant and susceptible lines of Brassica rapa L., 'G004' and 'Hakusai Chukanbohon Nou 7' (A9709), respectively, we identified two loci, Crr1 and Crr2, for clubroot (caused by Plasmodiophora brassicae Woronin) resistance. Each locus segregated independently among the F2 population, indicating that the loci reside on a different region of chromosomes or on different chromosomes. Genetic analysis showed that each locus had little effect on clubroot resistance by itself, indicating that these two loci are complementary for clubroot resistance. The resistance to clubroot was much stronger when both loci were homozygous for resistant alleles than when they were heterozygous. These results indicate that clubroot resistance in B. rapa is under oligogenic control and at least two loci are necessary for resistance.

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Acknowledgements

We thank Prof. Dr. Y. Kowyama, Dr. K. Kakeda and Dr. T. Tsuchiya of Mie University for their valuable advice. We are grateful to Ms. K. Tanaka and Ms. H. Maeda for their technical assistance. This work was supported by the Cooperative System for Supporting Priority Research of the Japan Science and Technology Corporation and by grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project DM-2105).

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Author notes

  1. M. Hirai

    Present address: Faculty of Agriculture, University of Kyoto prefecture, Kitainaazuma, Seika, Kyoto 619-0244, Japan,

Affiliations

  1. National Institute of Vegetable and Tea Science (NIVTS), 360 Kusawa, Ano, Age, Mie 514-2392, Japan

    K. Suwabe, H. Tsukazaki, K. Hatakeyama, M. Fujimura, T. Nunome, H. Fukuoka, S. Matsumoto & M. Hirai

  2. National Agricultural Research Organization, 3-1-1 Kannon-dai, Tsukuba, Ibaraki 305-8517, Japan

    H. Iketani

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  1. K. Suwabe
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Correspondence to S. Matsumoto.

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Communicated by H.C. Becker

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Suwabe, K., Tsukazaki, H., Iketani, H. et al. Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L.. Theor Appl Genet 107, 997–1002 (2003). https://doi.org/10.1007/s00122-003-1309-x

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Keywords

  • Brassica rapa
  • Clubroot
  • Microsatellites (SSRs)
  • Plasmodiophora brassicae
  • Disease resistance