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Linkage mapping of Hsa-1Og, a resistance gene of African rice to the cyst nematode, Heterodera sacchari

Abstract

Inheritance of resistance to cyst nematode (Heterodera sacchari) in Oryza sativa was investigated by inoculation tests with isolate 244 from Congo in segregating populations derived from hybridisation between O. sativa and its African sister cultivated species, O. glaberrima. We found that the resistance was controlled by one major gene, Hsa-1 Og, with codominance of susceptible and resistant alleles. To map Hsa-1 Og on the rice genome, we pooled the data obtained from segregation of the resistance trait and microsatellite markers in three kinds of progeny: BC1F3, BC1F4, and pseudo-F2 populations. Hsa-1 Og was unambiguously located between Cornell University's RM206 and RM254 markers on chromosome 11. Two additional microsatellite markers derived from Monsanto publicly available sequences were found to be tightly linked to the Hsa-1 Og gene. It is possible that numerous plant resistances to a pathogen in fact exhibit a codominant inheritance, possibly explaining misleading conclusions in several reports on resistance segregation.

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Acknowledgements

The authors thank the skilful technical assistance of V. Fenelon, L. Legagneur, and C. Sannier.

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Correspondence to M. Lorieux.

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Communicated by H.F. Linskens

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Lorieux, M., Reversat, G., Garcia Diaz, S.X. et al. Linkage mapping of Hsa-1Og, a resistance gene of African rice to the cyst nematode, Heterodera sacchari . Theor Appl Genet 107, 691–696 (2003). https://doi.org/10.1007/s00122-003-1285-1

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Keywords

  • Rice (Oryza sativa L., Oryza glaberrima Steud)
  • Cyst nematode (Heterodera sacchari)
  • Resistance gene
  • Molecular mapping