Abstract
In Japanese pear (Pyrus pyrifolia Nakai), fruit storage potential is closely related to the amount of ethylene produced. We have developed a rapid and accurate method for analyzing genes involved in high ethylene production during fruit ripening in Japanese pear. This involves cleaved-amplified polymorphic sequences (CAPS) of two 1-aminocyclopropane-1-carboxylate (ACC) synthase genes (PPACS1 and PPACS2). Two CAPS markers (A for PPACS1 and B for PPACS2), associated with the amount of ethylene produced, were identified. Marker A was associated with high ethylene producers and marker B with moderate ethylene producers. The absence of these two markers enabled the identification of low ethylene producers. Using these markers, we have identified ethylene genotypes for 40 Japanese pear cultivars and two Chinese pear (P. bretschneideri) cultivars that are commercially important and used in breeding programs. Furthermore, we performed linkage analysis of these two genes in the F2 population, which revealed that the recombination frequency between the two markers was 20.8 ± 3.6%. This information is critical to the selection of parents and in breeding strategies to improve storage ability of Japanese pears.
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References
Abeles FB (1992) Fruit ripening, abscission and postharvest disorders. In: Abeles FB, Morgan PW, Saltveit ME (eds) Ethylene in plant biology. Academic Press, San Diego, pp 182–221
Allard RW (1956) Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24:235–278
Brady CJ, Speirs J (1991) Ethylene in fruit ontogeny and abscission. In: Matto AK, Suttle JC (eds) The plant hormone ethylene. CRC Press, Boca Raton, pp 235–258
Hamilton AJ, Lycett GW, Grierson D (1990) Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants. Nature 346:284–287
Harada T, Sunako T, Sakuraba W, Goto S, Senda M, Akada S, Niizeki M (1997) Genomic nucleotide sequence of a ripening-related 1-aminocyclopropane-1-carboxylate synthase gene (MdACS-1) in apple. Plant Physiol 113:1465
Iketani H, Manabe T, Matsuta N, Akihama T, Hayashi T (1998) Incongruence between RFLPs of chloroplast DNA and morphological classification in east Asian pear (Pyrus spp.). Gene Resour Crop Evol 45:533–539
Iketani H, Abe K, Yamamoto T, Kotobuki K, Sato Y, Saito T, Terai O, Matsuta N, Hayashi T (2001) Mapping of disease-related genes in Japanese pear using a molecular linkage map with RAPD markers. Breed Sci 51:179–184
Itai A, Kawata T, Tanabe K, Tamura F, Uchiyama M, Tomomitsu M, Shiraiwa N (1999) Identification of 1-aminocyclopropane-1-carboxylic acid synthase genes controlling the ethylene level of ripening fruit in Japanese pear (Pyrus pyrifolia Nakai). Mol Gen Genet 262:42–49
Kajiura I (1981) Historical changes and geographical differentiations in harvest season of Japanese pear cultivars (Pyrus serotina Rehder var. culta Rehder). J Jpn Soc Hortic Sci 50:157–168
Kajiura I (1994) Nashi (Japanese pear). In: Konishi K, Iwahori S, Kitagawa H, Yakuwa T (eds) Horticulture in Japan. Asakura, Tokyo, pp 40–47
Kajiura I, Sato Y (1990) Recent progress in Japanese pear (Pyrus pyrifolia Nakai) breeding, and descriptions of cultivars based on literature review (in Japanese with English summary). Bull Fruit Res Stn Extra 1:97–329
Kende H (1993) Ethylene biosynthesis. Annu Rev Plant Physiol Plant Mol Biol 44:283–307
Kitamura T, Iwata T, Fukushima T, Furukawa T, Ishiguro T (1981) Studies on the maturation-physiology and storage of fruits and vegetables. II. Respiration and ethylene production in reference to species and cultivars of pear fruit (in Japanese with English summary). J Jpn Soc Hortic Sci 49:608–616
Knee M (1993) Pome fruits. In: Seymour GB, Taylor JE, Tucker GA (eds) Biochemistry of fruit ripening. Chapman and Hall, London, pp 325–346
Lay-Yee M, Knighton ML (1995) A full-length cDNA encoding 1-aminocyclopropane-1-carboxylate synthase from apple. Plant Physiol 107:1017–1018
Nakajima N, Mori H, Yamazaki K, Imaseki H (1990) Molecular cloning and sequence of a complementary DNA encoding 1-aminocyclopropane-1-carboxylate synthase induced by tissue wounding. Plant Cell Physiol 31:1021–1029
Oeller PW, Min-Wong L, Taylor LP, Pike DA, Theologis A (1991) Reversible inhibition of tomato fruit senescence by antisense RNA. Science 254:437–439
Sassa H, Hirano H, Nishio T, Koba T (1997) Style-specific self-compatible mutation caused by deletion of the S-RNase gene in Japanese pear (Pyrus serotina). Plant J 12:223–227
Sato T, Theologis A (1989) Cloning the mRNA encoding 1-aminocyclopropane -1-carboxylate synthase, the key enzyme for ethylene biosynthesis in plants. Proc Natl Acad Sci USA 86:6621–6625
Sunako T, Sakuraba W, Senda M, Akada S, Ishikawa R, Niizeki M, Harada T (1999) An allele of the ripening-specific 1-aminocyclopropane-1-carboxylic acid synthase gene (ACS1) in apple fruit with a long storage life. Plant Physiol 119:1297–1303
Teng Y, Tanabe K, Tamura F, Itai A (2001) Genetic relationships of pear cultivars in Xinjiang, China, as measured by RAPD markers. J Hortic Sci Biotechnol 76:771–779
Teramoto S, Kano-Murakami Y, Hori M, Kamiyama K (1994) 'DNA fingerprinting' to distinguish cultivar and parental relation of Japanese pear (in Japanese with English summary). J Jpn Soc Hortic Sci 63:17–21
Van Der Straeten D, Van Wiemeersch L, Goodman HM, Van Montagu M (1990) Cloning and sequence of two different cDNAs encoding 1-aminocyclopropane-1-carboxylate synthase in tomato. Proc Natl Acad Sci USA 87:4859–4863
Yamamoto T, Kimura T, Shoda M, Imai T, Saito T, Sawamura Y, Kotobuki K, Matsuta N, Hayashi T (2002) In: Genetic linkage maps of European and Japanese pears; 10th PAG Conf., San Diego [abstr P544]
Yang SF, Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol 35:155–189
Zheng XY, Wolff DW (2000) Ethylene production, shelf-life and evidence of RFLP polymorphisms linked to ethylene genes in melon (Cucumis melo L.). Theor Appl Genet 101:613–624
Acknowledgements
This study was supported by a Grant-in-Aid (no. 10760019) from the Ministry of Education, Science, Sports and Culture of Japan. We thank Prof. J.D. Bewley (University of Guelph, Canada) for his critical reading of the manuscript.
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Itai, A., Kotaki, T., Tanabe, K. et al. Rapid identification of 1-aminocyclopropane-1-carboxylate (ACC) synthase genotypes in cultivars of Japanese pear (Pyrus pyrifolia Nakai) using CAPS markers. Theor Appl Genet 106, 1266–1272 (2003). https://doi.org/10.1007/s00122-002-1186-8
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DOI: https://doi.org/10.1007/s00122-002-1186-8