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Development of rapeseed with high erucic acid content by asymmetric somatic hybridization between Brassica napus and Crambe abyssinica

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Abstract

PEG-induced asymmetric somatic hybridization between Brassica napus and Crambe abyssinica was carried out. C. abyssinica is an annual cruciferous oil crop with a high content of erucic acid in the seed oil valuable for technical purposes. UV-irradiated mesophyll protoplasts of C. abyssinica cv 'Carmen' and cv 'Galactica' were fused with hypocotyl protoplasts of different genotypes of B. napus cv 'Maplus' and breeding line '11502'. Shoot regeneration frequency varied between 6.1% and 20.8% among the different doses of UV-irradiation, ranging from 0.05 J/cm2 to 0.30 J/cm2. In total, 124 shoots were regenerated, of which 20 asymmetric somatic hybrids were obtained and verified by nuclear DNA content and AFLP analysis. AFLP data showed that some of the characteristic bands from C. abyssinica were present in the hybrids. Cytological analysis of these hybrids showed that 9 out of 20 asymmetric hybrids had 38 chromosomes, the others contained 40–78 chromosomes, having additional chromosomes between 2 and 40 beyond the 38 expected for B. napus. The investigation into the fertility of asymmetric somatic hybrids indicated that the fertility increased with increasing UV-doses ranging from 0.05 J/cm2 to 0.15 J/cm2. All of the hybrids were cultured to full maturity, and could be fertilized and set seeds after self-pollination or backcrosses with B. napus. An analysis of fatty acid composition in the seeds was conducted and found to contain significantly greater amounts of erucic acid than B. napus. This study indicates that UV-irradiation could be used as a tool to produce asymmetric somatic hybrids and to promote the fertility of the hybrids.

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Acknowledgements

We thank J. Gramenz for help with flow cytometry, Dr. B. Hackauf for instruction on AFLP analysis. This research was supported in part by a fellowship from the Biosciences Special Programme of DAAD, and a grant from Fachagentur Nachwachsende Rohstoffe (FNR).

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Authors and Affiliations

  1. Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, 18190 Groß Lüsewitz, Germany

    Y. P. Wang, K. Sonntag & E. Rudloff

  2. College of Life Sciences, Sichuan University, 610064 Chengdu, P.R. China

    Y. P. Wang

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  1. Y. P. Wang
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  2. K. Sonntag
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  3. E. Rudloff
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Correspondence to K. Sonntag.

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Communicated by K. Glimelius

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Wang, Y.P., Sonntag, K. & Rudloff, E. Development of rapeseed with high erucic acid content by asymmetric somatic hybridization between Brassica napus and Crambe abyssinica . Theor Appl Genet 106, 1147–1155 (2003). https://doi.org/10.1007/s00122-002-1176-x

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  • DOI: https://doi.org/10.1007/s00122-002-1176-x

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