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Molecular linkage maps of Vitis vinifera L. and Vitis riparia Mchx

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Abstract.

Two linkage maps for grape (Vitis spp.) have been developed based on 81 F1 plants derived from an interspecific cross between the wine cultivar Moscato bianco (Vitis vinifera L.) and a Vitis riparia Mchx. accession, a donor of pathogen resistance traits. The double pseudotest-cross mapping strategy was applied using three types of molecular markers. The efficiency of SSRs to anchor homologous linkage groups from different Vitis maps and the usefulness of AFLPs in saturating molecular linkage maps were evaluated. Moreover, the SSCP technique was developed based on sequence information in public databases concerning genes involved in flavonoid and stilbene biosynthesis. For the maternal genetic map a total of 338 markers were assembled in 20 linkage groups covering 1,639 cM, whereas 429 loci defined the 19 linkage groups of the paternal map which covers 1,518 cM. The identification of 14 linkage groups common to both maps was possible based on 21 SSR and 19 AFLP loci. The position of SSR loci in the maps presented here was consistent with other published mapping experiments in Vitis.

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Acknowledgements.

This research was partially funded by the Italian Ministry of Agriculture in the framework of the National research program on Plant Biotechnology (Area 2: Plant Genome – Molecular maps). VMC SSR markers were developed thanks to an International Consortium coordinated by Agrogene. We thank Alberto Madini and Sieglinde Effgen for their excellent assistance in the mapping work.

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Author notes

  1. C. Pozzi

    Present address: Fondazione Parco Tecnologico Padano, c/o DiProVe, Facoltà di Agraria, Università di Milano, via Celoria 2, 20133 Milano, Italy,

  2. R. Velasco

    Present address: Istituto Agrario di San Michele all'Adige, Via Mach 1 I38010 San Michele all'Adige, Trento, Italy,

Authors and Affiliations

  1. Istituto Agrario di San Michele all'Adige, Via Mach 1 – I38010 San Michele all'Adige, Trento, Italy

    M. S. Grando, D. Bellin & M. Stefanini

  2. IACR – Long Ashton, Long Ashton Research Station, Long Ashton, Bristol BS18 9AF, UK

    K. J. Edwards

  3. Max-Planck-Institut für Züchstungsforschung, Carl-von-Linne-Weg 10, 50829 Köln, Germany

    C. Pozzi & R. Velasco

Authors
  1. M. S. Grando
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  2. D. Bellin
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  3. K. J. Edwards
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  4. C. Pozzi
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  5. M. Stefanini
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  6. R. Velasco
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Correspondence to M. S. Grando.

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Communicated by F. Salamini

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Grando, M.S., Bellin, D., Edwards, K.J. et al. Molecular linkage maps of Vitis vinifera L. and Vitis riparia Mchx. Theor Appl Genet 106, 1213–1224 (2003). https://doi.org/10.1007/s00122-002-1170-3

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  • DOI: https://doi.org/10.1007/s00122-002-1170-3

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