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Development of PCR-based markers linked to dominant genes for male-fertility restoration in Pampa CMS of rye (Secale cereale L.)

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Abstract.

Cytoplasmatic male sterility (CMS) is the basis for commercial hybrid seed production of rye. Nuclear restorer genes are indispensable for a complete restoration of fertility of the CMS lines. The drawbacks of current European restorer lines require the utilisation of new genetic resources that have been recently detected in an Iranian primitive rye population (IRAN IX) and an Argentinean landrace (Pico Gentario). The introgression of these effective restorer genes (Rfp1 and Rfp2, respectively) into breeding material can be facilitated by marker-assisted selection. Using two F2 populations based on crosses between the non-restorer inbred line Lo6 and the restorer IRAN IX, as well as Pico Gentario, RAPDs and AFLPs were screened and led to a closely linked marker set for each of these genes. The conversion of the closest markers into fragment-specific sequence-characterised amplified region (SCAR) markers resulted in flanking ranges of 2.9 cM (Rfp1) and 5.2 cM (Rfp2). The application of these markers in backcross programmes is discussed.

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Acknowledgements.

The authors highly acknowledge the assistance and help of many colleagues who contributed to this study: the F2 population Lo6 × IRAN IX was generated by P. Wilde and B. Schmiedchen (Lochow-Petkus GmbH, Bergen); H. Wortmann (Hybro GmbH & Co KG, Bad Schönborn), P. Wilde, B. Schmiedchen and E. Knopf (Pflanzenzucht Dr. h.c. Carsten, Bad Schwartau) provided test sites and assisted in taking the anther scores. The AFLP marker screening was assessed by Keygene N.V. We are grateful to K. Dehmer (IPK, Gatersleben), V. Korzun (Lochow-Petkus GmbH, c/o PLANTA GmbH, Einbeck) and C. Knaak (Resistenzlabor SAATEN-UNION GmbH) for advice and valuable discussions concerning the presented studies, and T. Presterl (University of Hohenheim, Stuttgart) for helpful suggestions on the manuscript. The project was financially supported by the foregoing breeding companies and the Federal Ministry of Education and Research (BMB+F), Bonn, Germany (Grant no. 0311601).

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  1. S. Stracke

    Present address: Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, D-06466 Gatersleben, Germany,

Authors and Affiliations

  1. State Plant Breeding Institute, University of Hohenheim (720), D-70593 Stuttgart, Germany

    T. Miedaner

  2. Institute of Plant Breeding, Seed Science, and Population Genetics, University of Hohenheim (350), D-70593 Stuttgart, Germany

    S. Stracke, A. G. Schilling & H. H. Geiger

  3. PLANTA GmbH, Grimsehlstrasse 31, P.O. Box 1463, D-37555 Einbeck, Germany

    C. Weiss & C. Glass

  4. Saaten-Union Resistenzlabor GmbH, Hovedisser Strasse 92, D-33818 Leopoldshöhe, Germany

    J. Förster

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  1. S. Stracke
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  2. A. G. Schilling
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  3. J. Förster
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  4. C. Weiss
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  5. C. Glass
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  6. T. Miedaner
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  7. H. H. Geiger
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Correspondence to T. Miedaner.

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Communicated by H.C. Becker

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Stracke, S., Schilling, A.G., Förster, J. et al. Development of PCR-based markers linked to dominant genes for male-fertility restoration in Pampa CMS of rye (Secale cereale L.). Theor Appl Genet 106, 1184–1190 (2003). https://doi.org/10.1007/s00122-002-1153-4

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