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„Tissue engineering“ in der rekonstruktiven Urologie

Tissue engineering in reconstructive urology

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Zusammenfassung

Der Begriff „tissue engineering“ (TE) umfasst verschiedene Verfahren zur Herstellung von Ersatzgeweben und -organen. In der Urologie bietet TE v. a. für die Rekonstruktion der ableitenden Harnwege vielversprechende Möglichkeiten. Aktuell werden hauptsächlich Mundschleimhaut- und Urothelzellen für TE des Harntraktes verwendet. Verschiedene Materialien wurden hinsichtlich ihrer Eignung als Gewebematrizes untersucht. Neben den Methoden der Zellkultur und den Materialwissenschaften spielen die operativen Techniken der rekonstruktiven Urologie eine besondere Rolle für die erfolgreiche Implantation von TE-Transplantaten.

Abstract

The term tissue engineering incorporates various techniques for the production of replacement tissues and organs. In urology tissue engineering offers many promising possibilities for the reconstruction of the urinary tract. Currently, buccal mucosa and urothelial cells are most commonly used for tissue engineering of the urinary tract. Various materials have been tested for their suitability as tissue scaffolds. The ideal scaffold, however, has not yet been found. In addition to material sciences and cell culture methods, surgical techniques play an important role in reconstructive urology for the successful implantation of tissue engineered transplants.

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Literatur

  1. Barbagli G, Sansalone S, Romano G, Lazzeri M (2011) Controversies in urethral reconstruction. Minerva Urol Nefrol 63(3):251–260

    CAS  PubMed  Google Scholar 

  2. Rourke K, McKinny S, St. Martin B (2012) Effect of wound closure on buccal mucosal graft harvest site morbidity: results of a randomized prospective trial. Urology 79(2):443–447

    Article  PubMed  Google Scholar 

  3. Ahyai SA, Sayedahmed K, Engel O et al (2012) Harnableitung und Dickdarm. Urologe 51(7):937–946

    Article  CAS  PubMed  Google Scholar 

  4. Simaioforidis V, Jonge P de, Sloff M et al (2013) Ureteral tissue engineering: where are we and how to proceed? Tissue Eng Part B Rev 19(5):413–419

    Article  CAS  PubMed  Google Scholar 

  5. Krämer-Schultheiss KS, Schultheiss D (2002) Von der Wundheilung zum modernen Tissue Engineering der Haut. Hautarzt 53(11):751–760

    Article  PubMed  Google Scholar 

  6. Morrissey PE, Monaco AP (2014) Donation after circulatory death: current practices, ongoing challenges, and potential improvements. Transplantation 97(3):258–264

    Article  PubMed  Google Scholar 

  7. Atala A (2006) Recent applications of regenerative medicine to urologic structures and related tissues. Curr Opin Urol 16(4):305–309

    Article  PubMed  Google Scholar 

  8. Gucciardo L, Koninck P, Verfaillie C et al (2014) Perception and knowledge about stem cell and tissue engineering research: a survey amongst researchers and medical practitioners in perinatology. Stem Cell Rev Rep 10(4):1–8

    Article  Google Scholar 

  9. Kropp B (2014) The promise of tissue engineering in urology. J Urol 192(2):301–302. doi:10.1016/j.juro.2014.05.051

    Article  PubMed  Google Scholar 

  10. Atala A (2012) Tissue engineering of reproductive tissues and organs. Fertil Steril 98(1):21–29

    Article  CAS  PubMed  Google Scholar 

  11. Sievert K-D, Amend B, Stenzl A (2007) Tissue engineering for the lower urinary tract: a review of a state of the art approach. Eur Urol 52(6):1580–1589

    Article  PubMed  Google Scholar 

  12. Bhargava S, Patterson JM, Inman RD et al (2008) Tissue-engineered buccal mucosa urethroplasty – clinical outcomes. Eur Urol 53(6):1263–1271

    Article  PubMed  Google Scholar 

  13. Bolland F, Southgate J (2008) Bio-engineering urothelial cells for bladder tissue transplant. Expert Opin Biol Ther 8(8):1039–1049

    Article  CAS  PubMed  Google Scholar 

  14. Fu Q, Deng CL, Song XF, Xu YM (2008) Long-term study of male rabbit urethral mucosa reconstruction using epidermal cell. Asian J Androl 10(5):719–722

    Article  PubMed  Google Scholar 

  15. Fu Q, Song X-F, Liao G-L et al (2010) Myoblasts differentiated from adipose-derived stem cells to treat stress urinary incontinence. Urology 75(3):718–723

    Article  PubMed  Google Scholar 

  16. Zhang J, Gu GL, Liu GH et al (2012) Ureteral reconstruction using autologous tubular grafts for the management of ureteral strictures and defects: an experimental study. Urol Int 88(1):60–65

    Article  PubMed  Google Scholar 

  17. Orabi H, Lin G, Ferretti L et al (2012) Scaffoldless tissue engineering of stem cell derived cavernous tissue for treatment of erectile function. J Sex Med 9(6):1522–1534

    Article  CAS  PubMed  Google Scholar 

  18. Engel O, Ahyai S, Rink M et al (2013) Urethral reconstruction using buccal mucosa transplants. Urologe 52(5):650–656

    Article  CAS  PubMed  Google Scholar 

  19. Hölzle F, Mitchell DA, Rau A et al (2012) Assessment of the perfusion and morbidity of the buccal mucosal donor site for grafting of urethral strictures. J Craniomaxillofac Surg 40(1):47–50

    Article  PubMed  Google Scholar 

  20. Soave A, Steurer S, Dahlem R et al (2014) Histopathological characteristics of buccal mucosa transplants in humans after engraftment to the urethra: a prospective study. J Urol 192(6):1725–1729. doi:10.1016/j.juro.2014.06.089

    Article  PubMed  Google Scholar 

  21. Feil G, Maurer S, Nagele U et al (2008) Bioartifizielles autologes Urothel etabliert aus Spülungen der Harnblase. Urologe 47(9):1091–1096

    Article  CAS  PubMed  Google Scholar 

  22. Maurer S, Feil G, Stenzl A (2005) In vitro stratifiziertes Urothelium und seine Bedeutung für die rekonstruktive Urologie. Urologe 44(7):738–742

    Article  CAS  PubMed  Google Scholar 

  23. Wood D, Southgate J (2008) Current status of tissue engineering in urology. Curr Opin Urol 18(6):564–569

    Article  PubMed  Google Scholar 

  24. Schlote N, Wefer J, Sievert KD (2004) Azelluläre Matrix zur funktionellen Rekonstruktion des Urogenitaltraktes. Urologe A 43(10):1209–1212

    Article  CAS  PubMed  Google Scholar 

  25. Mangera A, Chapple CR (2013) Tissue engineering in urethral reconstruction – an update. Asian J Androl 15(1):89–92

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  26. Smith TG III, Gettman M, Lindberg G et al (2002) Ureteral replacement using porcine small intestine submucosa in a porcine model. Urology 60(5):931–934

    Article  PubMed  Google Scholar 

  27. Xie M, Xu Y, Song L et al (2014) Tissue-engineered buccal mucosa using silk fibroin matrices for urethral reconstruction in a canine model. J Surg Res 188(1):1–7

    Article  CAS  PubMed  Google Scholar 

  28. Royce PL, Zimmern P, deKernion JB (1988) Patch grafting the renal pelvis and ureteropelvic junction. A comparative study in pigs using lyophilized dura mater and free peritoneum. Urol Res 16(1):37–41

    Article  CAS  PubMed  Google Scholar 

  29. De Filippo RE, Kornitzer BS, Yoo JJ, Atala A (2012) Penile urethra replacement with autologous cell-seeded tubularized collagen matrices. J Tissue Eng Regen Med 89(3):358–364. doi:10.1002/term.1647

    Google Scholar 

  30. Brito-Juarez M, Engel O, De Petriconi R et al (2007) Ureterersatz durch ein „tissue-engineertes“ Veneninterponat. Urologe 46(9):1238

    Article  CAS  PubMed  Google Scholar 

  31. Brito-Juarez M, Volkmer BG, Gschwend JE et al (2007) Tissue engineered venous matrices for potential applications in the urogenital tract. Tissue Eng 13(10):2475–2482

    Article  CAS  PubMed  Google Scholar 

  32. Engel O, de Petriconi R, Volkmer BG et al (2014) The feasibility of ureteral tissue engineering using autologous veins: an orthotopic animal model with long term results. J Negat Results Biomed 13(1):17

    Article  PubMed Central  PubMed  Google Scholar 

  33. Mundy AR, Andrich DE (2011) Urethral strictures. BJU Int 107(1):6–26

    Article  PubMed  Google Scholar 

  34. Atala A, Guzman L, Retik AB (1999) A novel inert collagen matrix for hypospadias repair. J Urol 162(3 Pt 2):1148–1151

    Article  CAS  PubMed  Google Scholar 

  35. El Kassaby A, AbouShwareb T, Atala A (2008) Randomized comparative study between buccal mucosal and acellular bladder matrix grafts in complex anterior urethral strictures. J Urol 179(4):1432–1436

    Article  Google Scholar 

  36. Roux PJ le (2005) Endoscopic urethroplasty with unseeded small intestinal submucosa collagen matrix grafts: a pilot study. J Urol 173(1):140–143

    Article  PubMed  Google Scholar 

  37. Raya-Rivera A, Esquiliano DR, Yoo JJ et al (2011) Tissue-engineered autologous urethras for patients who need reconstruction: an observational study. Lancet 377(9772):1175–1182

    Article  PubMed Central  PubMed  Google Scholar 

  38. Atala A, Bauer SB, Soker S et al (2006) Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet 367(9518):1241–1246

    Article  PubMed  Google Scholar 

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Einhaltung ethischer Richtlinien

Interessenkonflikt. O. Engel, A. Soave, M. Rink, R. Dahlem, O. Hellwinkel, F.K. Chun und M. Fisch geben an, dass kein Interessenkonflikt besteht. Dr. med. Michael Rink wird durch das GEROK-Stipendium der Universität Hamburg gefördert. Dieser Beitrag beinhaltet keine Studien an Menschen oder Tieren.

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Authors and Affiliations

  1. Urologische Klinik und Poliklinik, Universitätsklinikum Hamburg-Eppendorf, Martinistraße 52, 20246, Hamburg, Deutschland

    O. Engel, A. Soave, M. Rink, R. Dahlem, F.K. Chun & M. Fisch

  2. Institut für Rechtsmedizin, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Deutschland

    O. Hellwinkel

Authors
  1. O. Engel
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  2. A. Soave
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  3. M. Rink
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  4. R. Dahlem
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  5. O. Hellwinkel
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  6. F.K. Chun
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  7. M. Fisch
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Correspondence to O. Engel.

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O. Engel und A. Soave haben gleichermaßen zu dieser Arbeit beigetragen.

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Engel, O., Soave, A., Rink, M. et al. „Tissue engineering“ in der rekonstruktiven Urologie. Urologe 54, 690–695 (2015). https://doi.org/10.1007/s00120-014-3714-y

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  • DOI: https://doi.org/10.1007/s00120-014-3714-y

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