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Pyrosequenzierung in der Uroonkologie

Anwendungsmöglichkeiten am Beispiel des Prostatakarzinoms

Pyrosequencing in uro-oncology

Applications in prostate cancer

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Zusammenfassung

Veränderungen in den DNA-Methylierungsmustern bestimmter Genpromotoren sowie Gensequenzmutationen kommen in der Karzinogenese eine entscheidende Rolle zu. Molekularbiologische Methoden wie die Pyrosequenzierung erlauben eine spezifische quantitative Untersuchung dieser epigenetischen und genetischen Modifikationen. Die Bedeutung derartiger Veränderungen für das Prostatakarzinom und die Funktion des Pyrosequezierens werden in dieser Übersicht auf der Basis einer Recherche in der PubMed-Literaturdatenbank beschrieben und erläutert. In der Uroonkologie wurden bisher nur wenige Studien zu diesen Themenbereichen publiziert, doch zeichnet sich ab, dass epigenetische Mechanismen und spezifische Gensequenzmutationen eine Bedeutung in der Pathogenese des Prostatakarzinoms haben und deren detaillierte Analysen damit zukünftig neue Perspektiven in der Diagnostik eröffnen könnten.

Abstract

Changes in the methylation pattern in particular gene promoters as well as genetic sequence mutations play an important role in carcinogenesis. Molecular methods like pyrosequencing provide the specific analysis of these epigenetic and genetic modifications. In this review the relevance of these alterations for prostate cancer and the function of pyrosequencing will be described and explained on the basis of a search of the PubMed literature database. At present, in uro-oncology only a few studies outlining methylation in prostate cancer and pyrosequencing have been published. Nevertheless, it becomes evident that epigenetic mechanisms as well as specific gene sequence alterations have an impact on the carcinogenesis of prostate cancer and knowledge of these factors might open perspectives in diagnostic approaches of the future.

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Authors and Affiliations

  1. Urologische Klinik und Poliklinik, Campus Großhadern, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Marchioninistraße 15, 81377, München, Deutschland

    T.C. Stadler, B. Schlenker, P. Nuhn, C.G. Stief & P.J. Bastian

  2. Pathologisches Institut, Ludwig-Maximilians-Universität München, München, Deutschland

    A. Jung & T. Kirchner

  3. Klinik und Poliklinik für Urologie, Universitätsklinikum Bonn, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, Deutschland

    J. Ellinger

Authors
  1. T.C. Stadler
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  2. A. Jung
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  3. B. Schlenker
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  4. P. Nuhn
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  5. J. Ellinger
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  6. T. Kirchner
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  7. C.G. Stief
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  8. P.J. Bastian
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Correspondence to T.C. Stadler.

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Stadler, T., Jung, A., Schlenker, B. et al. Pyrosequenzierung in der Uroonkologie. Urologe 49, 1356–1364 (2010). https://doi.org/10.1007/s00120-010-2426-1

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  • DOI: https://doi.org/10.1007/s00120-010-2426-1

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