Abstract
Endophytic actinomycetes, a prolific source of natural products, are well known for their diverse metabolic versatility, and their association with medicinal plants and antimicrobial potential are well worth exploring. We isolated and identified the Streptomyces cavourensis strain MH16 inhabiting the tree Millingtonia hortensis Linn. using phylogenetic analysis based on a 16S rRNA molecular approach. We used the disc diffusion method to evaluate the impact of differences in the compositions of the media on the production of secondary metabolites from strain MH16. The production of antimicrobial metabolites was determined by the observation of inhibition zones on intensive bands when using a TLC-bioautography assay. Biosynthesis of secondary metabolites was optimal when the strain MH16 was cultured in ISP-2 medium as depicted by a zone of inhibition. Strain MH16 effectively inhibited methicillin-resistant Staphylococcus aureus, Escherichia coli, Candida albicans, and other multi drug-resistant pathogens. The minimum inhibitory concentration of the antimicrobial metabolites was 25–100 μg mL−1. The study manifests the optimization and utilization of different fermentation media which best suits for increased production of the secondary metabolites from Streptomyces cavourensis. This research suggests that the antimicrobial metabolites of strain MH16 found in M. hortensis has great potential for the biodiscovery of new anti-infective drugs against a wide range of multidrug-resistant pathogens.
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Acknowledgments
The authors thank the Indian Council of Medical Research (ICMR), New Delhi, for the award of Senior Research Fellowship (No.3/2/2/65/2018/Online Onco Fship/NCD-III) to the first author. We are grateful to the Centre for Nano Science and Engineering (CeNSE), the Indian Institute of Science (IISc), Bengaluru, for the scanning electron microscopic analysis. The first author thanks Dr. R. Sundararaj, Scientist – G, Forest Protection Division, Institute of Wood Science and Technology for his support. We also thank the Department of Studies in Microbiology, University of Mysore, for providing laboratory and instrumentation facilities.
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Mondal, S., Rai, V.R. Molecular profiling of endophytic Streptomyces cavourensis MH16 inhabiting Millingtonia hortensis Linn. and influence of different culture media on biosynthesis of antimicrobial metabolites. Sci Nat 106, 51 (2019). https://doi.org/10.1007/s00114-019-1646-5
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DOI: https://doi.org/10.1007/s00114-019-1646-5