Abstract
Habitat destruction has resulted in the extinction of many plant species from the earth, and many more face extinction. Likely, the annual endemic Mediterranean knapweed (Centaurea tchihatcheffii) growing in the Golbasi district of Ankara, Turkey is facing extinction and needs urgent conservation. Plant tissue culture, a potentially useful technique for ex situ multiplication, was used for the restoration of this ill-fated plant through seed germination, micropropagation from stem nodes, and adventitious shoot regeneration from immature zygotic embryos. The seeds were highly dormant and very difficult to germinate. No results were obtained from the micropropagation of stem nodes. However, immature zygotic embryos showed the highest adventitious shoot regeneration on Murashige and Skoog (MS) medium, containing 1 mg l−1 kinetin and 0.25 mg l−1 NAA. Regenerated shoots were best rooted on MS medium containing 1 mg l−1 IBA and transferred to the greenhouse for flowering and seed set. As such, the present work is the first record of in vitro propagation of critically endangered C. tchihatcheffii, using immature zygotic embryos, and is a step forward towards conservation of this indigenous species.
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Abbreviations
- BAP:
-
6 benzylaminopurine
- CPA:
-
chlorophenoxyacetic acid
- IAA:
-
indole acetic acid
- IBA:
-
indole 3 butyric acid
- MS:
-
Murashige and Skoog medium
- NAA:
-
α naphthalene acetic acid
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Acknowledgements
The researchers are grateful to the University of Ankara and State Planning Commission-DPT for jointly supporting the studies (Grants through Project No. 98 K 120640 and 2001 K 120240) The authors also acknowledge the support of the Department of Biology Education, Gazi University, Ankara, Turkey and the Institute of Biotechnology, University of Ankara, Turkey for their support.
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Ozel, C.A., Khawar, K.M., Mirici, S. et al. Factors affecting in vitro plant regeneration of the critically endangered Mediterranean knapweed (Centaurea tchihatcheffii Fisch et. Mey). Naturwissenschaften 93, 511–517 (2006). https://doi.org/10.1007/s00114-006-0139-5
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DOI: https://doi.org/10.1007/s00114-006-0139-5