Abstract
The insect parasitoid Prorops nasuta has been introduced from Africa to many coffee-producing countries in an attempt to control the coffee berry borer. In this paper, we report on the sequencing of the ITS LSU-rDNA and beta-tubulin loci used to identify a fungus isolated from the cuticle of a P. nasuta that emerged from coffee berries infected with the coffee berry borer. The sequences were compared with deposits in GenBank and the fungus was identified as Aspergillus westerdijkiae. The fungus tested positive for ochratoxin A production, with varying levels depending on the media in which it was grown. These results raise the possibility that an insect parasitoid might be disseminating an ochratoxin-producing fungus in coffee plantations.
Explore related subjects
Discover the latest articles and news from researchers in related subjects, suggested using machine learning.References
Bennett JW, Klich M (2003) Mycotoxins. Clin Microbiol Rev 16:497–516
Bragulat MR, Abarca ML, Cabanes FJ (2001) An easy screening method for fungi producing ochratoxin A in pure culture. Int J Food Microbiol 71:139–144
Bustillo AE, Orozco J, Benavides P, Portilla M (1996) Producción masiva y uso de parasitoides para el control de la broca del café en Colombia. Revista Cenicafé 47:215–230
Bustillo AE, Cárdenas R, Villalba DA, Benavides P, Orozco J, Posada FJ (1998) Manejo integrado de la broca del café Hypothenemus hampei (Ferrari) en Colombia. Chinchiná, Cenicafé, 134 pp
Crowe TJ (1963) Possible insect vectors of the uredospores of Hemileia vastatrix in Kenya. Trans Br Mycol Soc 46:24–26
Dodd AP (1961) Biological control of Eupatorium adenophorum in Queensland. Aust J Sci 23:356–365
European Union (2005) Commission Regulation (EC) No 123/2005 of 26 January 2005 amending Regulation (EC) No 466/2001 as regards ochratoxin A. Off J Eur Union 48:3–5, (Legislation L 025, January 28, 2005)
Frisvad JC, Frank JM, Houbraken JAMP, Kuijpers AFA, Samson RA (2004) New ochratoxin A producing species of Aspergillus section Circumdati. Stud Mycol 50:23–43
Hargreaves H (1935) Stephanoderes hampei Ferr., coffee berry borer in Uganda. East Afr Agr J 1:218–224
Le Pelley RH (1968) Pests of coffee. Longmans Green, London, pp 590
Nemeye PS, Moore D, Prior C (1990) Potential of the parasitoid Heterospilus prosopidis (Hymenoptera: Braconidae) as a vector of plant-pathogenic Colletotrichum sp. Ann Appl Biol 116:11–19
Pérez J, Infante F, Vega FE, Holguín F, Macías J, Valle J, Nieto G, Peterson SW, Kurtzman CP, O’Donnell K (2003) Mycobiota associated with the coffee berry borer (Hypothenemus hampei) in Mexico. Mycol Res 107:879–887
Peterson SW, Vega FE, Posada F, Nagai C (2005) Penicillium coffeae, a new endophytic species isolated from a coffee plant and its phylogenetic relationship to P. fellutanum, P. thiersii, and P. brocae based on parsimony analysis of multilocus DNA sequences. Mycologia 97:659–666
Quintero C, Bustillo AE, Benavides P, Chaves B (1998) Evidencias del establecimiento de Cephalonomia stephanoderis y Prorops nasuta (Hymenoptera: Bethylidae) en cafetales del departamento de Nariño, Colombia. Rev Col Entomol 24:141–147
Vega FE, Mercadier G (1998) Insects, coffee, and ochratoxin A. Fla Entomol 81:543–544
Vega FE, Posada F, Peterson SW, Gianfagna TJ, Chaves F (2006) Penicillium species endophytic in coffee plants and ochratoxin A production. Mycologia (in press)
Waterston J (1923) Notes on parasitic Hymenoptera. Bull Entomol Res 14:103–118
Acknowledgements
We thank P. F. Dowd and M. Greenstone (USDA, ARS) and three anonymous reviewers for comments on a previous version of this manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Vega, F.E., Posada, F., Gianfagna, T.J. et al. An insect parasitoid carrying an ochratoxin producing fungus. Naturwissenschaften 93, 297–299 (2006). https://doi.org/10.1007/s00114-006-0101-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00114-006-0101-6