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Bohrmehl: Eine Quelle vitaler Zellen!

Erste Ergebnisse von humanen Proben

Reaming debris: a source of vital cells!

First results of human specimens

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Zusammenfassung

Bohrmehl fällt beim Aufbohren der Knochen im Rahmen einer Osteosynthese an. Bisher liegen jedoch nur widersprüchliche Informationen über seine Zusammensetzung vor. Allem voran bleibt zu klären, ob Bohrmehl noch vitale Zellen enthält. Ziel der vorliegenden Untersuchung war die praxisnahe Gewinnung von Zellen aus humanem Bohrmehl und ihre Charakterisierung. Dazu wurden 21 Bohrmehlproben elektronenmikroskopisch und in der Zellkultur untersucht.

Die gewonnenen Zellen wurden in osteogene, chondrogene und adipogene Differenzierungsmedien verbracht. Darüber hinaus wurden FACS-Analysen durchgeführt. Ultrastrukturell waren sowohl intakte als auch zerstörte Zellen nachweisbar. In der Zellkultur ließen sich aus allen Proben Zellen anzüchten. Diese konnten aufgrund ihrer Morphologie, ihres Differenzierungsverhaltens sowie ihres Antigenprofils als mesenchymale Stammzellen charakterisiert werden. Damit belegen die vorliegenden Befunde erstmals, dass humanes Bohrmehl eine Quelle vitaler Stammzellen ist, und eröffnen die Möglichkeit, Bohrmehl als Alternative zur Spongiosaplastik einzusetzen.

Abstract

Reaming debris is generated in the course of intramedullary reaming of long bones. Up to now there has been little information about the composition of reaming debris. Especially, it remains to be elucidated if reaming debris contains vital cells. The goal of the present vitro investigation has been the harvest of cells from human reaming debris and the subsequent characterization of the cells. 21 specimens of human reaming debris have been investigated. Each specimen has been divided into two parts. One part has been examined by means of transmission electron microscopy while the other part of each specimen has been transferred into culture dishes. The developing cell cultures were characterized by using FACS analysis and were incubated within osteogenic, adipogenic and chondrogenic differentiation media. The results of electron microscopy have revealed the presence of both, vital cells and massively altered cells. Cell growth occurred after initial plating of all specimens. The cells which were grown within the culture dishes could be characterized as mesenchymal stem cells on the basis of their morphology, differentiation capacity and antigen profile. Based upon these results reaming debris has to be regarded as a source of vital mesenchymal stem cells. It remains to be elucidated, if reaming debris can be used as an alternative to bone tissue grafting.

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Authors and Affiliations

  1. Labor für Experimentelle Unfallchirurgie, Universitätsklinikum Gießen

    K. Trinkaus & S. Wenisch

  2. Klinik und Poliklinik für Unfallchirurgie, Universitätsklinikum Gießen

    C. Siemers & R. Schnettler

  3. Medizinische Klinik V und Poliklinik, Universitätsklinikum Heidelberg

    D. Hose

  4. Labor für Experimentelle Unfallchirurgie, Universitätsklinikum Gießen, Kerkrader Straße 9, 35394 , Gießen

    K. Trinkaus

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  1. K. Trinkaus
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  2. S. Wenisch
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  3. C. Siemers
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  4. D. Hose
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  5. R. Schnettler
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Correspondence to K. Trinkaus.

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Trinkaus, K., Wenisch, S., Siemers, C. et al. Bohrmehl: Eine Quelle vitaler Zellen!. Unfallchirurg 108, 650–656 (2005). https://doi.org/10.1007/s00113-005-0960-x

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  • DOI: https://doi.org/10.1007/s00113-005-0960-x

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