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Knochenheilung in Bohrlochdefekten spontandiabetischer BB/OK-Ratten

Eine histomorphometrische und biomechanische Studie

Bone healing in drill defects in spontaneously diabetic BB/OK rats

A histomorphometric and biomechanical study

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Zusammenfassung

Ziel der Studie war die histomorphometrische und biomechanische Abschätzung des Einflusses der diabetischen Stoffwechsellage auf die Knochendefektheilung in Abhängigkeit von der Defektgröße bei spontandiabetischen BB/OK-Ratten. Anhand der Blutglukosespiegel und des Insulinbedarfs postoperativ wurden 120 spontandiabetische BB/OK-Ratten mit guter und schlechter diabetischer Stoffwechselführung gruppiert. 60 LEW.1A-Ratten dienten als normoglykämische Kontrolltiere. Knochendefekte unterschiedlicher Größe im distalen Femur wurden nach 2, 4 und 6 Wochen Heilung untersucht. Ausschließlich bei den Ratten mit schlechter diabetischer Stoffwechsellage zeigte sich bei einer Defektgröße ab 0,8 mm eine signifikante Abnahme struktureller Kalkulationen und bei einer Defektgröße ab 1,2 mm signifikant verringerte fluorochromabhängige Parameter für die Mineralisation, sowie eine signifikante Abnahme der biomechanischen Eigenschaften im Vergleich zu den Ratten mit guter diabetischer Stoffwechselführung und den Kontrollratten. Die Knochendefektheilung kleiner Defekte ist unabhängig von der diabetischen Stoffwechsellage spontandiabetischer BB/OK-Ratten. Mit kontrollierter Insulintherapie und resultierender guter diabetischer Stoffwechsellage können die schweren histomorphometrischen und biomechanischen Störungen bei der Knochendefektheilung größerer Defekte mit schlechter diabetischer Stoffwechsellage verhindert werden.

Abstract

The objective of this study was to examine changes in the histomorphometric and mechanical evaluation of the influence of the diabetic metabolic state on defect healing depending on the defect size in spontaneously diabetic BB/O(ttawa)K(arlsburg) rats. Based on blood glucose levels and postoperative insulin requirements, 120 spontaneously diabetic BB/OK rats were divided into groups with well-compensated or poorly compensated metabolic state. Sixty LEW.1A rats served as the normoglycemic controls. Bone defects of different sizes were created proximal to the knee joint space and then allowed to heal for 2, 4, and 6 weeks. Bone defect sizes ≥0.8 mm showed significant differences in the structural calculations and bone defect sizes ≥1.2 mm showed a significant decrease of all fluorochrome-based parameters of mineralization exclusively in the rats with poorly compensated diabetic metabolic state as well as significantly decreased values of biomechanical properties in comparison to the spontaneously diabetic rats with well-compensated metabolic states and to the control rats. Bone repair of minor bone defects is independent of the diabetic metabolic state in the spontaneously diabetic BB/OK rats. With controlled insulin therapy and resultant well-compensated diabetic metabolic state, severe mineralization and biomechanical disorders in healing of larger bone defects in the poorly compensated diabetic metabolic state of the experimental animal can be avoided.

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  1. Forschungslabor, Klinik und Poliklinik für Orthopädie und Orthopädische Chirurgie, Ernst-Moritz-Arndt-Universität, Greifswald

    N. Follak & H. Merk

  2. Abteilung für Versuchstierkunde, Institut für Pathophysiologie, Ernst-Moritz-Arndt-Universität, Greifswald

    I. Klöting

  3. Forschungslabor, Klinik und Poliklinik für Orthopädie und Orthopädische Chirurgie, Ernst-Moritz-Arndt-Universität, Sauerbruchstraße, 17487, Greifswald

    N. Follak

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  1. N. Follak
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Correspondence to N. Follak.

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Follak, N., Klöting, I. & Merk, H. Knochenheilung in Bohrlochdefekten spontandiabetischer BB/OK-Ratten. Unfallchirurg 107, 750–760 (2004). https://doi.org/10.1007/s00113-004-0803-1

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