Suppl. Fig. 2

In vitro effects of macrolide antibiotics on different Th subset differentiation and Th17 proliferation. a ROR gamma t expression in CD4⁺ T cells treated with the indicated macrolide concentrations at day 2 of Th17 culture. b Frequencies of FoxP3 expressing cells at day 4 of iTreg culture. c Frequencies of T-bet expressing cells and d IFN gamma producing CD4⁺ T cells at day 4 of Th1 culture. e Cell violet™ staining of Th17 differentiated CD4⁺ T cells at day 4 of culture. Data shown are representative of at least two individual experiments with three technical replicates each and depicted as mean ± S.E.M. Two-tailed, unpaired Student’s t test was used to determine significance between means of groups. ns (not significantly different); *p < 0.05 (PNG 4469 kb)