Correction to: Journal of Molecular Medicine (2018) 96:1061–1079

Fig. 7
figure 1

Mitochondrial electron transport complex and mitochondrial Ca2+ levels. a Total NADH oxidase activity and rotenone-sensitive activity was assessed from differentiated SH-SY5Y cell lysates (WT and STIM1-KO). Data are presented as the mean ± s.d. of two independent experiments. Right panel shows the difference between total activity and the remaining activity after rotenone addition to the assay, i.e., the rotenone-sensitive NADH oxidase. b Wild-type and STIM1-KO cells were transiently transfected for the expression of the Ca2+ sensor 4mtD3cpv and 48 h later emission of fluorescence was recorded for CFP, FRET (left and middle panels,), and YFP channels to monitor photobleaching. FRET/CFP ratio signal (right panel) was recorded for cells in Ca2+-containing HBSS for 4–5 min. Calibration of FRET/CFP ratio to calculate Rmin and Rmax was performed individually for every assay. [Ca2+]m data are presented as the mean ± s.d. of seven independent experiments

Fig. 8
figure 2

Increased cellular Ca2+ influx underlies mitochondrial failure and augmented senescence. a Changes in cytosolic-free Ca2+ concentration were analyzed in fura-2-loaded cells. Cells in HBSS containing 1.26 mM Ca2+ were subjected to 1-min depolarization with 90 mM KCl (red line). CaCl2 in the HBSS was increased to 5 mM during depolarization to facilitate the Ca2+ influx recording. In parallel experiments, 10 μM nifedipine was added to the assay medium during the recording (black line). Right panel: the increase of the F340/F380 ratio triggered by 90 mM KCl in the presence of VOCCs blockers is shown as mean ± s.d. of 3 experiments (a minimum of 70 cells per experimental condition). Final concentrations: 10 μM nifedipine, 1 μM ω-conotoxin MVIIC, 3 μM ML 218. b STIM1-KO cells, or STIM1-KO cells stably expressing a specific shRNA to knock-down CACNA1C transcripts, were treated as described in panel (a). The left panel shows a representative experiment, and the bar chart of the right panel shows the increase in the F340/F380 ratio evoked by depolarization (mean ± s.d. of two independent experiments; n > 60 cells per condition). c Senescence (left panel) and mitochondrial polarization (middle and right panels) were assessed from differentiated cells after 6 DIV, staining with C12FDG as described in Fig. 5c and TMRM as in Fig. 6b–d, respectively. Data are mean ± s.d. of three independent experiments (number of replicates is shown for each condition). d Rotenone-sensitive NADH oxidase activity was assessed from differentiated SH-SY5Y cell lysates (wild-type, STIM1-KO, and STIM1-KO + shRNA for CACNA1C). Data are presented as the mean ± s.d. of two independent experiments. e Cell were transiently transfected for the expression of the Ca2+ sensor 4mtD3cpv. Mitochondrial [Ca2+] was assessed as described in Fig. 7. Data of six independent experiments are shown in the right panel bar chart as mean ± s.d.

https://doi.org/10.1007/s00109-018-1677-y

The original publication of this paper contains errors.

Page 1066, first column, penultimate lane: where the text says "Ca2+ = 0.76 mM", it should say "Ca2+ = 0.76 µM"

Page 1070, second column, two last lanes: where the text says "27.8 ± 22.8 µM for KO vs 66.4 ± 10.9 µM for WT", it should say "27.8 ± 22.8 nM for KO vs 66.4 ± 10.9 nM for WT"

Page 1073, y-axis in the bar chart of the panel b: where the text says “Mitochondrial [Ca2+] (µM)” it should say “Mitochondrial [Ca2+] (nM)”

Page 1074, y-axis in the bar chart of the panel e: where the text says “Mitochondrial [Ca2+] (µM)” it should say “Mitochondrial [Ca2+] (nM)”

Page 1074, second column, fifth lane: where the text says "46 µM", it should say "46 nM"

Because the y-axis label in two different bar chart should be corrected, we provide the full figures with the corrected labels.