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Far infrared promotes wound healing through activation of Notch1 signaling

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Abstract

The Notch signaling pathway is critically involved in cell proliferation, differentiation, development, and homeostasis. Far infrared (FIR) has an effect that promotes wound healing. However, the underlying molecular mechanisms are unclear. In the present study, we employed in vivo and HaCaT (a human skin keratinocyte cell line) models to elucidate the role of Notch1 signaling in FIR-promoted wound healing. We found that FIR enhanced keratinocyte migration and proliferation. FIR induced the Notch1 signaling pathway in HaCaT cells and in a microarray dataset from the Gene Expression Omnibus database. We next determined the mRNA levels of NOTCH1 in paired normal and wound skin tissues derived from clinical patients using the microarray dataset and Ingenuity Pathway Analysis software. The result indicated that the Notch1/Twist1 axis plays important roles in wound healing and tissue repair. In addition, inhibiting Notch1 signaling decreased the FIR-enhanced proliferation and migration. In a full-thickness wound model in rats, the wounds healed more rapidly and the scar size was smaller in the FIR group than in the light group. Moreover, FIR could increase Notch1 and Delta1 in skin tissues. The activation of Notch1 signaling may be considered as a possible mechanism for the promoting effect of FIR on wound healing. FIR stimulates keratinocyte migration and proliferation. Notch1 in keratinocytes has an essential role in FIR-induced migration and proliferation. NOTCH1 promotes TWIST1-mediated gene expression to assist wound healing. FIR might promote skin wound healing in a rat model.

Key messages

  • FIR stimulates keratinocyte migration and proliferation.

  • Notch1 in keratinocytes has an essential role in FIR-induced migration and proliferation.

  • NOTCH1 promotes TWIST1-mediated gene expression to assist wound healing.

  • FIR might promote skin wound healing in a rat model.

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Acknowledgments

This study was supported by the Taipei Medical University-Shuang Ho Hospital (106TMU-SHH-07).

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Authors and Affiliations

  1. Division of Nephrology, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei, Taiwan

    Yung-Ho Hsu, Cheng-Hsien Chen, Yu-Jhe Chiu & Hui-Wen Chiu

  2. Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan

    Yung-Ho Hsu & Cheng-Hsien Chen

  3. Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, 250 Wuxing Street, Taipei, 110, Taiwan

    Yuan-Feng Lin & Hui-Wen Chiu

  4. Division of Nephrology, Department of Internal Medicine, Wan Fang Hospital, Taipei Medical University Taipei, Taipei, Taiwan

    Cheng-Hsien Chen

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  1. Yung-Ho Hsu
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Correspondence to Hui-Wen Chiu.

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Hsu, YH., Lin, YF., Chen, CH. et al. Far infrared promotes wound healing through activation of Notch1 signaling. J Mol Med 95, 1203–1213 (2017). https://doi.org/10.1007/s00109-017-1580-y

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  • DOI: https://doi.org/10.1007/s00109-017-1580-y

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