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Protektion der Kochlea durch Vitamin C bei Lärmexposition

Protection of the cochlea by ascorbic acid in noise trauma

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Zusammenfassung

Hintergrund

Die protektive Wirkung von Vitamin C bei lärminduzierter Hörminderung ist ebenso vom Tiermodell bekannt wie eine gesteigerte Stickstoffmonoxid(NO)-Bildung nach Beschallung. Unklar ist der Einfluss von Vitamin C auf die lärminduzierte NO-Bildung in der Kochlea.

Methoden

Vitamin C wurde für 7 Tage in niedriger (25 mg/kg Körpergewicht/Tag) und hoher (525 mg/kg Körpergewicht/Tag) Dosis bei Meerschweinchen (n=48) zugefüttert. Jeweils die Hälfte der Tiere wurde danach beschallt (90 dB, 1 h). Die Hörschwelle wurde vor Vitamingabe, am 3. und am 7. Tag nach Fütterungsbeginn sowie direkt nach der Beschallung aufgezeichnet. Danach wurden Corti-Organ und Lateralwand aus der Bulla entnommen, separat für 6 h in Kulturmedium inkubiert und anschließend der Nitritgehalt im Überstand bestimmt.

Ergebnisse

Bei hoher Vitamin-C-Zufütterung konnte der Hörverlust nach Beschallung um 8 dB reduziert werden. Eine Korrelation zwischen verbessertem Hörvermögen und reduzierter NO-Bildung war für beide Kochleaanteile erkennbar, in der Lateralwand aber stärker ausgeprägt.

Schlussfolgerung

In hoher Dosis kann Vitamin C die NO-Bildung im Innenohr bei Lärmexposition reduzieren, senkt die Hörschwelle ab und schützt die Kochlea vor nitroaktivem Stress.

Abstract

Background

The protective effect of ascorbic acid against noise-induced hearing loss and increased nitric oxide (NO) formation after noise exposure have already been demonstrated in animal models. However, the influence of ascorbic acid on noise-induced NO production within the cochlea is still unclear.

Methods

Guinea pigs (n=48) were fed for 7 days with low [25 mg/kg bodyweight (bw)/day] and high (525 mg/kg bw/day) doses of ascorbic acid. Then half of the animals were exposed to noise (90 dB for 1 h). The hearing levels were recorded beforehand, on the 3rd and 7th days after feeding, and directly after noise exposure. Finally, the organ of Corti and the lateral wall were removed from the inner ear and incubated separately for 6 h in culture medium, and the nitrite content was determined in the supernatant.

Results

Compared with low-dose feeding, feeding of high doses of ascorbic acid resulted in a reduction of hearing impairment of about 8 dB after noise exposure. A correlation between hearing improvement and decreased NO production was detectable for both cochlea regions but was more pronounced in the lateral wall.

Conclusion

A high dose of ascorbic acid lowers NO production in the inner ear, reduces hearing loss, and protects the cochlea from nitroactive stress.

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Literatur

  1. Allaerts W, Koopman WJH, Verlaan BPJ et al. (2000) Endogenous production of nitric oxide and effects of nitric oxide and superoxide on melanotrope functioning in the pituitary pars intermedia of Xenopus laevis. Nitric Oxide 4: 15–28

    Article  PubMed  CAS  Google Scholar 

  2. Evans P, Halliwell B (1999) Free radicals and hearing. Cause, consequence and criteria. Ann N Y Acad Sci 884: 19–40

    Article  PubMed  CAS  Google Scholar 

  3. Heinrich UR, Selivanova O, Feltens R et al. (2005) Endothelial nitric oxide synthase up-regulation in the guinea pig organ of corti after acute noise trauma. Brain Res 1047: 85–96

    Article  PubMed  CAS  Google Scholar 

  4. Heinrich UR, Fischer I, Brieger J et al. (2008) Ascorbic acid reduces noise-induced nitric oxide production in the guinea pig ear. Laryngoscope 118: 837–842

    Article  PubMed  CAS  Google Scholar 

  5. Le Prell CG, Hughes LF, Miller JM (2007) Free radical scavengers vitamins A, C and E plus magnesium reduce noise trauma. Free Radic Biol Med 42: 1454–1463

    Article  CAS  Google Scholar 

  6. May JM (2000) How does ascorbic acid prevent endothelial dysfunction? Free Radic Biol Med 28: 1421–1429

    Article  PubMed  CAS  Google Scholar 

  7. McFadden SL, Woo JM, Michalak N et al. (2005) Dietary vitamin C supplementation reduces noise-induced hearing loss in guinea pigs. Hear Res 202: 200–208

    Article  PubMed  CAS  Google Scholar 

  8. Meister A (1994) Glutathione-ascorbic acid antioxidant system in animals. J Biol Chem 269: 9397–9400

    PubMed  CAS  Google Scholar 

  9. Muriel P, Sandoval G (2000) Nitric oxide and peroxynitrite anion modulate liver plasma membrane fluidity and Na+/K+-ATPase activity. Nitric Oxide 4: 333–342

    Article  PubMed  CAS  Google Scholar 

  10. Ohinata Y, Yamasoba T, Schacht J et al. (2000) Glutathione limits noise-induced hearing loss. Hear Res 146: 28–34

    Article  PubMed  CAS  Google Scholar 

  11. Rice ME (2000) Ascorbate regulation and its neuroprotective role in the brain. Trends Neurosci 23: 209–216

    Article  PubMed  CAS  Google Scholar 

  12. Rümelin A, Dörr S, Heinzel U et al. (2003) Stability of ascorbic acid in several blood collecting procedures. J Nutr Environ Med 13: 87–89

    Article  Google Scholar 

  13. Shi X, Nuttall AL (2003) Upregulated iNOS and oxidative damage to the cochlear stria vascularis due to noise stress. Brain Res 967: 1–10

    Article  PubMed  CAS  Google Scholar 

  14. Shi X, Ren T, Nuttall AL (2002) The electrochemical and fluorescence detection of nitric oxide in the cochlea and its increase following loud sound. Hear Res 164: 49–58

    PubMed  CAS  Google Scholar 

  15. Shi X, Dai C, Nuttall AL (2003) Altered expression of inducible nitric oxide synthase (iNOS) in the cochlea. Hear Res 177: 43–52

    Article  PubMed  CAS  Google Scholar 

  16. Terril LA, Clemons DJ (1998) The laboratory guinea pig. CRC Press, Boca Raton, FL

  17. Wangemann P (2006) Supporting sensory transduction: cochlear fluid homeostasis and the endocochlear potential. J Physiol 576: 11–21

    Article  PubMed  CAS  Google Scholar 

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Danksagung

Die Autoren danken Frau K. Backes für ihre hochgeschätzte technische Unterstützung.

Interessenkonflikt

Der korrespondierende Autor gibt an, dass kein Interessenkonflikt besteht.

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Authors and Affiliations

  1. Hals-Nasen-Ohrenklinik und Poliklinik, Johannes-Gutenberg-Universität Mainz, Langenbeckstraße 1, 55131, Mainz, Deutschland

    I. Fischer, U.-R. Heinrich, J. Brieger, W.J. Mann &  K. Helling

  2. Institut für Medizinische Biometrie, Epidemiologie und Informatik (IMBEI), Johannes-Gutenberg-Universität Mainz, Mainz, Deutschland

    I. Schmidtmann

  3. Institut für Pharmokologie, Johannes-Gutenberg-Universität Mainz, Mainz, Deutschland

    H. Li

  4. Klinik für Anästhesiologie, Johannes-Gutenberg-Universität Mainz, Mainz, Deutschland

    A. Rümelin

Authors
  1. I. Fischer
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  2. U.-R. Heinrich
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  3. J. Brieger
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  4. I. Schmidtmann
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  5. H. Li
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  6. A. Rümelin
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  7. W.J. Mann
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  8. K. Helling
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Corresponding author

Correspondence to K. Helling.

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Fischer, I., Heinrich, UR., Brieger, J. et al. Protektion der Kochlea durch Vitamin C bei Lärmexposition. HNO 57, 339–344 (2009). https://doi.org/10.1007/s00106-008-1826-7

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  • DOI: https://doi.org/10.1007/s00106-008-1826-7

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