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Konstitutive Expression des potentiellen Stammzellmarkers CD44 in permanenten HNSCC-Zelllinien

Constitutive expression of the potential stem cell marker CD44 in permanent HNSCC cell lines

HNO volume 56, pages 461–466 (2008)Cite this article

Zusammenfassung

Hintergrund

Trotz der steten Entwicklung neuer Diagnose- und Behandlungsmethoden konnte die Überlebensrate von Patienten mit Plattenepithelkarzinomen im Kopf-Hals-Bereich („head and neck squamous cell carcinoma“, HNSCC) in den vergangenen Jahrzehnten nicht entscheidend verbessert werden. Dies unterstreicht die dringende Notwendigkeit der Entwicklung innovativer immuntherapeutischer Ansätze. In diesem Zusammenhang wird zunehmend deutlich, dass Stammzellen an der Entstehung und Progression von Tumoren maßgeblich beteiligt sind. Deren Identität sowie die zugrunde liegenden zellulären und molekularen Mechanismen sind jedoch bei Kopf-Hals-Karzinomen weitestgehend unbekannt.

Material und Methoden

Tumoren des Kopf-Hals-Bereichs sowie die Zellen des peripheren Bluts von Patienten mit HNSCC wurden mittels Durchflusszytometrie hinsichtlich der Expression verschiedener potentieller Stammzellmarker untersucht.

Ergebnisse

Es konnten distinkte CD44-positive, lin-negative Zellpopulationen innerhalb der HNSCC-Einzelzellsuspensionen identifiziert werden, jedoch mit starken individuellen Abweichungen. Überraschenderweise zeigen unsere Untersuchungen, dass der potentielle Stammzellmarker CD44 konstitutiv von allen untersuchten permanenten HNSCC-Zelllinien auf der Zelloberfläche exprimiert wird.

Schlussfolgerungen

Unsere Daten lassen stark vermuten, dass CD44-positive Tumorstammzellen möglicherweise auch eine Schlüsselrolle bei der Etablierung permanenter HNSCC-Zelllinien spielen. Dabei findet eine Selektion besonders robuster Zellpopulationen statt, die im wirklichen Leben möglicherweise die Progression und Metastasierung von HNSCC vorantreiben. Weitere Stammzellmarker werden hinsichtlich ihrer Expression und Funktion untersucht werden.

Abstract

Background

Despite significant advances and the use of new diagnosic and therapy methods to treat head and neck squamous cell carcinoma (HNSCC), prognosis has improved only marginally in the last decades. Thus, there is an enormous need for novel immunotherapeutic approaches. It is becoming more and more obvious that stem cells play an important role in tumor development and progression. The identity of these cells and the underlying cellular and molecular mechanisms remain mostly unknown in HNSCC.

Material and methods

Solid tumors as well as cells from the peripheral blood of patients with HNSCC were analyzed by flow cytometry concerning the expression of different putative stem cell marker proteins.

Results

Distinct populations of CD44-positive (CD44+), lin-negative (lin-) potential stem cells could be identified in solid tumors of HNSCC patients with strong individual variations. Surprisingly, the potential stem cell marker CD44 was found to be constitutively expressed on the surface of all permanent HNSCC cell lines analyzed.

Conclusion

These data strongly suggest that CD44+ tumor stem cells may play a key role in establishment of permanent HNSCC cell lines, selecting especially robust cell entities that in real life might drive progression and metastasis of HNSCC. Individual analysis of tumor stem cell markers will be an important tool for innovative therapies and prognosis of patients with HNSCC. Additional stem cell markers will be investigated concerning their expression and cellular function.

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Affiliations

  1. Klinik für HNO und Plastische Operationen, Universitätsklinikum Schleswig Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Deutschland

    R. Pries, N. Wittkopf, K. Hasselbacher &  B. Wollenberg

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  1. R. Pries
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  2. N. Wittkopf
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  3. K. Hasselbacher
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  4. B. Wollenberg
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Correspondence to B. Wollenberg.

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Pries, R., Wittkopf, N., Hasselbacher, K. et al. Konstitutive Expression des potentiellen Stammzellmarkers CD44 in permanenten HNSCC-Zelllinien. HNO 56, 461–466 (2008). https://doi.org/10.1007/s00106-008-1707-0

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  • DOI: https://doi.org/10.1007/s00106-008-1707-0

Schlüsselwörter

  • Kopf-Hals-Karzinom
  • Tumorstammzelle
  • Immuntherapie

Keywords

  • Head and neck cancer
  • Tumor stem cells
  • Immunotherapy