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Genomnachweis von Dermatophyten

Aktuelle Erkenntnisse aus dem Ringversuch

Genome detection of dermatophytes

Current findings from the external quality assessment test

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Zusammenfassung

Für die Diagnostik bei Verdacht auf Dermatophytosen sind neben der Kulturdiagnostik seit einiger Zeit auch molekulargenetische Verfahren verfügbar. Seit März 2016 bietet INSTAND e. V. in Zusammenarbeit mit dem Nationalen Konsiliarlabor für Dermatophyten 2‑mal jährlich einen Ringversuch für den Genomnachweis von Dermatophyten an. Mehr als die Hälfte der Teilnehmer dieses Ringversuchs verwenden für die Analyse der Ringversuchsproben kommerzielle Kits. Proben, die Trichophyton rubrum oder keinen Dermatophyten enthielten, wurden von allen Kits mit hoher Sicherheit korrekt bestimmt. Bei der Speziesdiagnostik über Trichophyton rubrum hinaus gibt es große Unterschiede zwischen den einzelnen Kits. Diese werden hier anhand von klinischen Studien und den Ergebnissen aus dem Ringversuch näher beleuchtet.

Abstract

In addition to morphological diagnostics, molecular methods have become available for the diagnosis of suspected dermatophytoses. Since March 2016, INSTAND e. V., in cooperation with the National Reference Laboratory for Dermatophytes, has provided an external quality assessment (EQA) test for the genome detection of dermatophytes twice a year. More than half of the participants used commercial kits for the analysis of the samples. All kits with a high level of accuracy correctly determined the presence of Trichophyton rubrum or no dermatophytes. In species diagnostics beyond Trichophyton rubrum there are large differences between the kits. These are examined in more detail based on clinical studies and the results of the EQA test.

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Correspondence to Y. Gräser.

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C. Kupsch und Y. Gräser geben an, dass kein Interessenkonflikt besteht.

Für diesen Beitrag wurden von den Autoren keine Studien an Menschen oder Tieren durchgeführt.

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Kupsch, C., Gräser, Y. Genomnachweis von Dermatophyten. Hautarzt 70, 627–637 (2019). https://doi.org/10.1007/s00105-019-4435-3

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