Skip to main content
SpringerLink
Log in

Genomnachweis von Dermatophyten

Aktuelle Erkenntnisse aus dem Ringversuch

Genome detection of dermatophytes

Current findings from the external quality assessment test

  • Leitthema
  • Published:
Der Hautarzt Aims and scope Submit manuscript

Zusammenfassung

Für die Diagnostik bei Verdacht auf Dermatophytosen sind neben der Kulturdiagnostik seit einiger Zeit auch molekulargenetische Verfahren verfügbar. Seit März 2016 bietet INSTAND e. V. in Zusammenarbeit mit dem Nationalen Konsiliarlabor für Dermatophyten 2‑mal jährlich einen Ringversuch für den Genomnachweis von Dermatophyten an. Mehr als die Hälfte der Teilnehmer dieses Ringversuchs verwenden für die Analyse der Ringversuchsproben kommerzielle Kits. Proben, die Trichophyton rubrum oder keinen Dermatophyten enthielten, wurden von allen Kits mit hoher Sicherheit korrekt bestimmt. Bei der Speziesdiagnostik über Trichophyton rubrum hinaus gibt es große Unterschiede zwischen den einzelnen Kits. Diese werden hier anhand von klinischen Studien und den Ergebnissen aus dem Ringversuch näher beleuchtet.

Abstract

In addition to morphological diagnostics, molecular methods have become available for the diagnosis of suspected dermatophytoses. Since March 2016, INSTAND e. V., in cooperation with the National Reference Laboratory for Dermatophytes, has provided an external quality assessment (EQA) test for the genome detection of dermatophytes twice a year. More than half of the participants used commercial kits for the analysis of the samples. All kits with a high level of accuracy correctly determined the presence of Trichophyton rubrum or no dermatophytes. In species diagnostics beyond Trichophyton rubrum there are large differences between the kits. These are examined in more detail based on clinical studies and the results of the EQA test.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abb. 1
Abb. 2
Abb. 3
Abb. 4

Literatur

  1. Bergman A, Heimer D, Kondori N, Enroth H (2013) Fast and specific dermatophyte detection by automated DNA extraction and real-time PCR. Clin Microbiol Infect 19:E205–E211

    Article  CAS  PubMed  Google Scholar 

  2. Bontems O, Hauser PM, Monod M (2009) Evaluation of a polymerase chain reaction-restriction fragment length polymorphism assay for dermatophyte and nondermatophyte identification in onychomycosis. Br J Dermatol 161:791–796

    Article  CAS  PubMed  Google Scholar 

  3. Brillowska-Dabrowska A, Saunte DM, Arendrup MC (2007) Five-hour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum. J Clin Microbiol 45:1200–1204

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Gräser Y, Czaika V, Ohst T (2012) Diagnostic PCR of dermatophytes—an overview. J Dtsch Dermatol Ges 10:721–725

    PubMed  Google Scholar 

  5. Hayette M‑P, Seidel L, Adjetey C et al (2019) Clinical evaluation of the DermaGenius® Nail real-time PCR assay for the detection of dermatophytes and Candida albicans in nails. Med Mycol 57(3):277–283. https://doi.org/10.1093/mmy/myy020

    Article  PubMed  Google Scholar 

  6. Kosanke S, Harder M, Brasch J et al (2017) Tinea infections—a novel and rapid detection of causative fungal agents with a DNA-based Microarray. Mycoses 60:17–17

    Google Scholar 

  7. Kosanke S, Harder M, Brasch J et al (2017) Dermatomycosis—a novel and rapid detection of causative fungal agents with a DNA-based Microarray (EUROarray Dermatomycosis). J Mol Diagn 19:978–978

    Google Scholar 

  8. Kroschwald geb. Mehlig L, Weber C, Mareen A et al (2015) Rapid DNA-probe based screening assay for differential diagnosis of dermatomycoses. Mycoses 58:63–64

    Article  Google Scholar 

  9. Kupsch C, Berlin M, Gräser Y (2017) Dermatophyten und Meerschweinchen. Hautarzt 68:827–830

    Article  CAS  PubMed  Google Scholar 

  10. Kupsch C, Ohst T, Pankewitz F et al (2016) The agony of choice in dermatophyte diagnostics—performance of different molecular tests and culture in the detection of Trichophyton rubrum and Trichophyton interdigitale. Clin Microbiol Infect 22:735.e11–735.e17

    Article  CAS  Google Scholar 

  11. Litz CE, Cavagnolo RZ (2010) Polymerase chain reaction in the diagnosis of onychomycosis: a large, single-institute study. Br J Dermatol 163:511–514

    Article  CAS  PubMed  Google Scholar 

  12. Mehlig L, Garve C, Ritschel A et al (2014) Clinical evaluation of a novel commercial multiplex-based PCR diagnostic test for differential diagnosis of dermatomycoses. Mycoses 57:27–34

    Article  CAS  PubMed  Google Scholar 

  13. Nenoff P, Uhrlaß S, Krüger C et al (2014) Trichophyton Spezies von Arthroderma benhamiae – ein neuer Infektionserreger in der Dermatologie. J Dtsch Dermatol Ges 12:571–582

    PubMed  Google Scholar 

  14. Ohst T, Kupsch C, Gräser Y (2016) Detection of common dermatophytes in clinical specimens using a simple quantitative real-time TaqMan polymerase chain reaction assay. Br J Dermatol 174:602–609

    Article  CAS  PubMed  Google Scholar 

  15. Petinataud D, Berger S, Contet-audonneau N, Machouart M (2014) Molecular diagnosis of onychomycosis. J Mycol Médicale 24:287–295

    Article  CAS  Google Scholar 

  16. Petinataud D, Berger S, Ferdynus C et al (2016) Optimising the diagnostic strategy for onychomycosis from sample collection to FUNGAL identification evaluation of a diagnostic kit for real-time PCR. Mycoses 59:304–311

    Article  CAS  PubMed  Google Scholar 

  17. Sherman S, Goshen M, Treigerman O et al (2018) Evaluation of multiplex real-time PCR for identifying dermatophytes in clinical samples—A multicentre study. Mycoses 61:119–126

    Article  CAS  PubMed  Google Scholar 

  18. Spiliopoulou A, Bartzavali C, Jelastopulu E et al (2015) Evaluation of a commercial PCR test for the diagnosis of dermatophyte nail infections. J Med Microbiol 64:25–31

    Article  CAS  PubMed  Google Scholar 

  19. Uhrlaß S, Ebert A, Krüger C, Nenoff P (2013) Trichophyton Spezies von Arthroderma benhamiae–ein neuer häufiger zoophiler Dermatophyt in Deutschland–Daten zur Prävalenz im mitteldeutschen Raum. Derm 19:370–372

    Google Scholar 

  20. Uhrlaß S, Krüger C, Bezold G, Nenoff P (2015) Direktnachweis von Dermatophyten-DNA mit PCR bei Nagelerkrankungen. derm 21:470–485

    Google Scholar 

  21. Wisselink GJ, van Zanten E, Kooistra-Smid AMD (2011) Trapped in keratin; a comparison of dermatophyte detection in nail, skin and hair samples directly from clinical samples using culture and real-time PCR. J Microbiol Methods 85:62–66

    Article  CAS  PubMed  Google Scholar 

  22. Wittig F, Uhrlass S, Gaajetaan G et al (2018) Detection and identification of pathogenic dermatophytes using multiplex real-time PCR assays. Med Mycol 56:S82–S82

    Google Scholar 

  23. Zink A, Papanagiotou V, Todorova A et al (2014) Outbreak of Microsporum audouinii in Munich—the return of infectious fungi in Germany. Mycoses 57:765–770

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Mikrobiologie und Infektionsimmunologie, Charité – Universitätsmedizin Berlin, Hindenburgdamm 27, 12203, Berlin, Deutschland

    C. Kupsch & Y. Gräser

Authors
  1. C. Kupsch
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Gräser
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Y. Gräser.

Ethics declarations

Interessenkonflikt

C. Kupsch und Y. Gräser geben an, dass kein Interessenkonflikt besteht.

Für diesen Beitrag wurden von den Autoren keine Studien an Menschen oder Tieren durchgeführt.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kupsch, C., Gräser, Y. Genomnachweis von Dermatophyten. Hautarzt 70, 627–637 (2019). https://doi.org/10.1007/s00105-019-4435-3

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00105-019-4435-3

Schlüsselwörter

Keywords

Search

Navigation