Skip to main content
SpringerLink
Log in

Charakterisierung der Interaktion zwischen Candida albicans und Wirtszellen

In-vitro-Modelle auf der Basis von rekonstituierter humaner Haut/Schleimhaut

Characterization of the interaction between Candida albicans and host cells

In vitro model using reconstituted human skin and mucosa

  • Leitthema
  • Published:
Der Hautarzt Aims and scope Submit manuscript

Zusammenfassung

Die mikrobiologische Grundlagenforschung benötigt geeignete Modellsysteme, um infektionsbiologische Fragestellungen in sinnvoller Weise bearbeiten zu können. Zum gegenwärtigen Zeitpunkt erfolgen solche Infektionsstudien meist im Rahmen von Tierversuchen, was aus ethischen Gründen aber umstritten ist. Eine mögliche Alternative, gerade für lokalisierte Infektionen, bieten Modelle auf der Basis von in vitro rekonstituiertem humanem Epithel oder rekonstituierter humaner Epidermis (RHE). In den letzten Jahren wurden diese Modellsysteme erfolgreich zur Evaluierung der Wirksamkeit topischer Antiinfektiva, zur Charakterisierung von fungalen Virulenzfaktoren und zur Beschreibung der Immunantwort bei lokalisierten Candida-albicans-Infektionen eingesetzt. Erst kürzlich wurden diese Modelle um weitere infektionsrelevante Immunzellen wie Lymphozyten, polymorphkernige Leukozyten, Mastzellen oder dendritische Zellen erweitert um deren Einfluss auf den Infektionslauf und die Interaktion zwischen Hautbarrieren und akzessorischen Immunzellen zu charakterisieren. Obgleich für RHE-Modelle die größte Erfahrung bei Candida-albicans-Infektionen besteht, können solche Modellsysteme auch für Infektionen mit anderen Pilzen oder auch Bakterien eingesetzt werden.

Abstract

Basic research on the biology and immunology of microbial infection requires appropriate model systems. Currently most such studies involve animal studies which are a focus of ethical controversy. Possible alternatives, especially for localized infections, are provided by models using in vitro reconstituted human epithelium or epidermis (RHE). In recent years, these model systems have been successfully established to evaluate the effectiveness of topical anti-infectives, to characterize the role of fungal virulence factors, and to study the immune responses during localized Candida albicans infections. Most recently, these models have been supplemented with immune cells such as lymphocytes, polymorphonuclear leukocytes, mast cells or dendritic cells, to study their role during the course of infection and to characterize the interaction between the skin barrier and accessory immune cells. Although the most experience is with Candida albicans RHE infections, such model systems can also be used to study infections with other fungi or bacteria.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abb. 1
Abb. 2
Abb. 3
Abb. 4
Abb. 5

Literatur

  1. Ruhnke M (2002) Skin and mucous membrane infections. In: Calderone RA (ed) Candida and candidiasis. ASM Press, Washington, DC, pp 307–325

  2. Ruhnke M (2006) Epidemiology of Candida albicans infections and role of non-Candida-albicans yeasts. Curr Drug Targets 7: 495–504

    Article  PubMed  CAS  Google Scholar 

  3. Naglik JR, Fidel PL jr, Odds FC (2008) Animal models of mucosal Candida infection. FEMS Microbiol Lett 283: 129–139

    Article  PubMed  CAS  Google Scholar 

  4. Rahman D, Mistry M, Thavaraj S et al. (2007) Murine model of concurrent oral and vaginal Candida albicans colonization to study epithelial host-pathogen interactions. Microbes Infect 9: 615–622

    Article  PubMed  CAS  Google Scholar 

  5. Collins LM, Dawes C (1987) The surface area of the adult human mouth and thickness of the salivary film covering the teeth and oral mucosa. J Dent Res 66: 1300–1302

    PubMed  CAS  Google Scholar 

  6. Scully C, el-Kabir M, Samaranayake LP (1994) Candida and oral candidosis: a review. Crit Rev Oral Biol Med 5: 125–157

    PubMed  CAS  Google Scholar 

  7. Osmanski CP, Meyer J (1967) Differences in the fine structure of the mucosa of mouse cheek and palate. J Invest Dermatol 48: 309–317

    PubMed  CAS  Google Scholar 

  8. Dongari-Bagtzoglou A, Kashleva H, Villar CC (2004) Bioactive interleukin-1alpha is cytolytically released from Candida albicans-infected oral epithelial cells. Med Mycol 42: 531–541

    Article  PubMed  CAS  Google Scholar 

  9. Dongari-Bagtzoglou A, Kashleva H (2003) Candida albicans triggers interleukin-8 secretion by oral epithelial cells. Microb Pathog 34: 169–177

    Article  PubMed  CAS  Google Scholar 

  10. Dieterich C, Schandar M, Noll M et al. (2002) In vitro reconstructed human epithelia reveal contributions of Candida albicans EFG1 and CPH1 to adhesion and invasion. Microbiology 148: 497–506

    PubMed  CAS  Google Scholar 

  11. Dongari-Bagtzoglou A, Kashleva H (2006) Development of a novel three-dimensional in vitro model of oral Candida infection. Microb Pathog 40: 271–278

    Article  PubMed  CAS  Google Scholar 

  12. Schaller M, Korting HC, Schäfer W et al. (1999) Secreted aspartic proteinase (Sap) activity contributes to tissue damage in a model of human oral candidosis. Mol Microbiol 34: 169–180

    Article  PubMed  CAS  Google Scholar 

  13. Schaller M, Schackert C, Korting HC et al. (2000) Invasion of Candida albicans correlates with expression of secreted aspartic proteinases during experimental infection of human epidermis. J Invest Dermatol 114: 712–717

    Article  PubMed  CAS  Google Scholar 

  14. Schaller M, Schäfer W, Korting HC, Hube B (1998) Differential expression of secreted aspartyl proteinases in a model of human oral candidosis and in patient samples from the oral cavity. Mol Microbiol 29: 605–615

    Article  PubMed  CAS  Google Scholar 

  15. Schaller M, Zakikhany K, Naglik JR et al. (2006) Models of oral and vaginal candidiasis based on in vitro reconstituted human epithelia. Nat Protoc 1: 2767–2773

    Article  PubMed  CAS  Google Scholar 

  16. Schaller M, Borelli C, Korting HC, Hube B (2005) Hydrolytic enzymes as virulence factors of Candida albicans. Mycoses 48: 365–377

    Article  PubMed  CAS  Google Scholar 

  17. Calderone RA, Fonzi WA (2001) Virulence factors of Candida albicans. Trends Microbiol 9: 327–335

    Article  PubMed  CAS  Google Scholar 

  18. Schaller M, Korting HC, Borelli C et al. (2005) Candida albicans-secreted aspartic proteinases modify the epithelial cytokine response in an in vitro model of vaginal candidiasis. Infect Immun 73: 2758–2765

    Article  PubMed  CAS  Google Scholar 

  19. Schaller M, Preidel H, Januschke E, Korting HC (1999) Light and electron microscopic findings in a model of human cutaneous candidosis based on reconstructed human epidermis following the topical application of different econazole formulations. J Drug Target 6: 361–372

    Article  PubMed  CAS  Google Scholar 

  20. Hahn BL, Sohnle PG (1988) Characteristics of dermal invasion in experimental cutaneous candidiasis of leucopenic mice. J Invest Dermatol 91: 233–237

    Article  PubMed  CAS  Google Scholar 

  21. Schaller M, Mailhammer R, Grassl G et al. (2002) Infection of human oral epithelia with Candida species induces cytokine expression correlated to the degree of virulence. J Invest Dermatol 118: 652–657

    Article  PubMed  CAS  Google Scholar 

  22. Dongari-Bagtzoglou A, Villar CC, Kashleva H (2005) Candida albicans-infected oral epithelial cells augment the anti-fungal activity of human neutrophils in vitro. Med Mycol 43: 545–549

    Article  PubMed  CAS  Google Scholar 

  23. Schaller M, Boeld U, Oberbauer S et al. (2004) Polymorphonuclear leukocytes (PMNs) induce protective Th1-type cytokine epithelial responses in an in vitro model of oral candidosis. Microbiology 150: 2807–2813

    Article  PubMed  CAS  Google Scholar 

  24. Weindl G, Naglik JR, Kaesler S et al. (2007) Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling. J Clin Invest 117: 3664–3672

    PubMed  CAS  Google Scholar 

  25. Schaller M, Laude J, Bodewaldt H et al. (2004) Toxicity and antimicrobial activity of a hydrocolloid dressing containing silver particles in an ex vivo model of cutaneous infection. Skin Pharmacol Physiol 17: 31–36

    Article  PubMed  CAS  Google Scholar 

Download references

Interessenkonflikt

Der korrespondierende Autor gibt an, dass kein Interessenkonflikt besteht.

Author information

Authors and Affiliations

  1. Universitäts-Hautklinik Tübingen, Eberhard Karls Universität Tübingen, Liebermeisterstraße 25, 72076, Tübingen, Deutschland

    M. Schaller & G. Weindl

  2. Abteilung Mikrobielle Pathogenität, Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie, Hans Knoell-Institut, Friedrich Schiller Universität Jena, Jena, Deutschland

    B. Hube

  3. Klinik und Poliklinik für Dermatologie und Allergologie, Ludwig-Maximilians-Universität München, München, Deutschland

    C. Borelli & H.C. Korting

  4. Karolinska Institutet, Department of MicrobiologyTumor and Cell Biology Stockholm, Sweden, Stockholm, Schweden

    K. Zakikhany

Authors
  1. M. Schaller
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Zakikhany
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Weindl
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Borelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H.C. Korting
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. B. Hube
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Schaller.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schaller, M., Zakikhany, K., Weindl, G. et al. Charakterisierung der Interaktion zwischen Candida albicans und Wirtszellen. Hautarzt 59, 964–970 (2008). https://doi.org/10.1007/s00105-008-1549-4

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00105-008-1549-4

Schlüsselwörter

Keywords

Search

Navigation