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Local fixation of antibiotics with fibrin spray on soft tissues: experimental study on the relevance of the application techniques

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European Journal of Trauma and Emergency Surgery Aims and scope Submit manuscript

Abstract

Objective

In acute and chronic infections of soft tissue, a radical surgical debridement is necessary, and is regarded as the ‘Gold Standard, as well as an immediately systemic antibiotic therapy. Additional treatment with local antibiotics and/or antibiotic-containing materials is commonly used in clinical settings, but the efficient concentration of the antibiotic is not well-known.

Methods

Aside from the typical procedures which are provided and supported by the industry, we have conducted an animal study including 39 Sprague Dawley rats. We have treated the back sores with local antibiotics (vancomycin) alone or in two combinations with a fibrin–glue spray technique.

Results

It could be demonstrated that, in particular, the simultaneous application of vancomycin and fibrin leads to a stable antibiotic concentration and a long period of time wherein the antibiotic substance remains in the tissue.

Conclusions

This easy and inexpensive method of application can be a promising factor for the clinical improvement of local antibiotic therapy.

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Acknowledgements

This work was in part financially supported by Baxter Deutschland GmbH. R.V. and M.J. performed the animal experiments. S.F. performed the measurements. I.M., J.F. and M.J. were involved in planning and supervised the work. R.V. processed the experimental data and designed the figures. I.M. and J.F. aided in interpreting the results. I.M., J.F., R.V., S.F. and M.J. worked on the manuscript. All authors discussed the results and commented on the manuscript.

Funding

This study was in part funded by Baxter Deutschland GmbH.

Author information

Authors and Affiliations

  1. Department of Trauma, Hand and Reconstructive Surgery, Hospital of the Goethe-University, Theodor-Stern-Kai 7, 60590, Frankfurt am Main, Germany

    René Verboket, Ingo Marzi, Johannes Frank & Maren Janko

  2. Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Branch for Translational Medicine and Pharmacology TMP, Theodor-Stern-Kai 7, 60596, Frankfurt am Main, Germany

    Stefanie C. Fleck

Authors
  1. René Verboket
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  2. Ingo Marzi
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  3. Stefanie C. Fleck
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  4. Johannes Frank
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  5. Maren Janko
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Corresponding author

Correspondence to Maren Janko.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The animal experiments were performed in accordance with the regulations set forth by our institution’s animal care and oversight committee (Project No. FK/1045; Regierungspräsidium, Darmstadt, Germany) in accordance with German law.

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Verboket, R., Marzi, I., Fleck, S.C. et al. Local fixation of antibiotics with fibrin spray on soft tissues: experimental study on the relevance of the application techniques. Eur J Trauma Emerg Surg 46, 635–640 (2020). https://doi.org/10.1007/s00068-018-1054-0

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  • DOI: https://doi.org/10.1007/s00068-018-1054-0

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