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Plasma osteopontin levels, but not its myocardial expression, reflect heart failure severity in recently diagnosed dilated cardiomyopathy

Plasmawerte von Osteopontin, jedoch nicht dessen myokardiale Expression, zeigen den Schweregrad einer Herzinsuffizienz bei kürzlich diagnostizierter dilatativer Kardiomyopathie

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Abstract

Background

Elevated levels of the extracellular matrix glycoprotein osteopontin (OPN) may be detected in both myocardium and plasma under various pathological conditions affecting the heart. Several studies demonstrated increased plasma OPN levels in patients with heart failure due to dilated cardiomyopathy (DCM), while other studies showed high OPN expression levels in the myocardium of such patients. However, very little is known about OPN levels in both plasma and myocardium of the same individual with DCM. Therefore, we aimed to compare plasma OPN levels and levels of myocardial OPN expression in patients with recent-onset DCM (Ro-DCM).

Methods

We examined plasma OPN as well as creatinine, C‑reactive protein (CRP), brain natriuretic peptide (BNP), and troponin I levels in 25 patients with Ro-DCM. Furthermore, all subjects underwent transthoracic echocardiography, selective coronary angiography, and endomyocardial biopsy (EMB) for the assessment of myocardial OPN expression.

Results

No significant correlation between myocardial OPN expression and clinical, biochemical, or echocardiographic parameters was found. In log transformation analysis, plasma OPN levels correlated significantly with BNP levels (r = 0.46, p = 0.031), with CRP levels (r = 0.52, p = 0.015), and with early diastolic mitral annular velocity (r = −0.57, p = 0.009). There was a borderline association between the plasma OPN log value and New York Heart Association class (p = 0.053).

Conclusion

Plasma OPN levels reflect heart failure severity in patients with Ro-DCM. Myocardial OPN expression is not associated with either plasma OPN levels or markers of heart failure in these individuals.

Zusammenfassung

Hintergrund

Erhöhte Spiegel des extrazellulären Matrixglykoproteins Osteopontin (OPN) sind bei verschiedenen pathologischen Veränderungen des Herzens sowohl im Myokard als auch im Plasma nachweisbar. In mehreren Studien wurden bei Patienten mit Herzinsuffizienz aufgrund dilatativer Kardiomyopathie (DCM) erhöhte OPN-Plasmaspiegel festgestellt, während sich in anderen Studien hohe OPN-Expressionswerte im Myokard solcher Patienten zeigten. Jedoch ist nur sehr wenig über die OPN-Werte sowohl im Plasma als auch im Myokard desselben Patienten mit DCM bekannt. Daher war es Ziel der Studie, die OPN-Plasmawerte und die Werte der myokardialen OPN-Expression bei Patienten mit seit Kurzem bestehender DCM („recent onset DCM“, Ro-DCM) zu vergleichen.

Methoden

Dazu wurden die Werte für OPN im Plasma, Kreatinin, C‑reaktives Protein (CRP), BNP („brain natriuretic peptide“) und Troponin I bei 25 Patienten mit Ro-DCM bestimmt. Außerdem erfolgte bei allen Teilnehmern eine transthorakale Echokardiographie, selektive Koronarangiographie und Endomyokardbiopsie (EMB) zur Bestimmung der myokardialen OPN-Expression.

Ergebnisse

Zwischen myokardialer OPN-Expression und klinischen, biochemischen oder echokardiographischen Parametern fand sich keine signifikante Korrelation. In der Log-Transformationsanalyse waren die OPN-Werte im Plasma signifikant mit den BNP-Werten (r = 0,46; p = 0,031), den CRP-Werten (r = 0,52; p = 0,015) und der frühdiastolischen Mitralringgeschwindigkeit korreliert (r = −0,57; p = 0,009). Ein grenzwertiger Zusammenhang bestand zwischen dem Plasma-OPN-Log-Wert und der Klasse nach New York Heart Association (p = 0,053).

Schlussfolgerung

Die OPN-Werte im Plasma widerspiegeln den Schweregrad der Herzinsuffizienz bei Patienten mit Ro-DCM. Dagegen ist die myokardiale OPN-Expression weder mit den OPN-Werten im Plasma noch mit Markern der Herzinsuffizienz bei diesen Patienten assoziiert.

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Acknowledgements

The authors are thankful to Ms. Hana Rehakova form the Institute of Medical Biochemistry and Laboratory Diagnostics for technical assistance

Funding

The study was supported by MH CZ DRO 64165, Progres Q25 and Q38/LF1 projects.

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Authors and Affiliations

  1. 2nd Department of Medicine—Department of Cardiovascular Medicine, First Faculty of Medicine, Charles University and General University Hospital in Prague, U Nemocnice 2, 128 08, Prague 2, Czech Republic

    J. Podzimkova M.D., T. Palecek M.D., Ph.D., P. Kuchynka M.D., PhD., J. Marek M.D., B. A. Danek M.D. & A. Linhart M.D., Ph.D.

  2. Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic

    M. Jachymova M.D., Ph.D., M. Safarikova Ph.D., M. Kalousova M.D., Ph.D. & T. Zima M.D., Ph.D.

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  1. J. Podzimkova M.D.
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Correspondence to T. Palecek M.D., Ph.D..

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Conflict of interest

J. Podzimkova, T. Palecek, P. Kuchynka, J. Marek, B.A. Danek, M. Jachymova, M. Safarikova, M. Kalousova, T. Zima, and A. Linhart declare that they have no competing interests.

All studies performed were in accordance with the ethical standards indicated in each case.

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Podzimkova, J., Palecek, T., Kuchynka, P. et al. Plasma osteopontin levels, but not its myocardial expression, reflect heart failure severity in recently diagnosed dilated cardiomyopathy. Herz 45 (Suppl 1), 105–110 (2020). https://doi.org/10.1007/s00059-019-4829-0

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