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Planta

, Volume 203, Issue 1, pp 129–132 | Cite as

A succinate-oxidising nitrate reductase is located at the plasma membrane of plant roots

  • Christine Stöhr
  • Wolfram R. Ullrich
Rapid communication

Abstract.

Plasma-membrane-bound nitrate reductase (PM-NR) is located in roots and leaves of tobacco (Nicotiana tabacum L. cv. Samsun) and reduces nitrate with NADH as electron donor. When plasma membranes were prepared under specific protecting conditions, a PM-NR of roots was detected that accepts electrons from succinate to reduce nitrate. Comparison between the succinate dehydrogenase of mitochondria and the succinate-oxidising PM-NR of roots indicated that they are two different enzymes. Partial purification of the nitrate reductase forms by anion-exchange chromatography indicated that succinate and NADH supply electrons to the same plasma-membrane-bound protein.

Key words: Mitochondrion Nicotiana (nitrate reduc tase) Nitrate reductase (plasma-membrane-bound) Succinate Succinate dehydrogenase 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Christine Stöhr
    • 1
  • Wolfram R. Ullrich
    • 1
  1. 1.Institut füt Botanik, Technische Hochschule, Schnittspahnstr. 10, D-64287 Darmstadt, GermanyDE

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