Chemoecology

, Volume 22, Issue 2, pp 131–138 | Cite as

o-Coumaric acid from invasive Eupatorium adenophorum is a potent phytotoxin

Research Paper

Abstract

A phytochemical study of the invasive Eupatorium adenophorum indicated that the plant was rich in a phenolic compound o-coumaric acid (or 2-hydroxycoumaric acid). Biological investigations with the model plant Arabidopsis thaliana and crop plants showed that o-coumaric acid strongly inhibited seed germination, plant growth and root elongation, reduced the photosynthesis in old leaves, and induced the root cell death and the expression of genes related to senescence, oxidative stress, and systemic acquired resistance. The phytotoxic effects of o-coumaric acid exhibit selectivity between under- and above-ground parts of test plants and between E. adenophorum and other plants. These results indicate that o-coumaric acid is a potent toxin that might play an important role in the competition of E. adenophorum with its neighboring plants during its invasion and establishment.

Keywords

Phytotoxin Arabidopsis thaliana Germination inhibition Growth inhibition Root cell death Leaf senescence 

Supplementary material

49_2012_105_MOESM1_ESM.doc (17.4 mb)
Supplementary material 1 (DOC 17,783 kb)

References

  1. Aliotta G, Cafiero G, Fiorentino A, Strumia S (1993) Inhibition of radish germination and root growth by coumarin and phenylpropanoids. J Chem Ecol 19:175–183CrossRefGoogle Scholar
  2. Baruah NC, Sarma JC, Sarma S, Sharma RP (1994) Seed germination and growth inhibitory cadinenes from Eupatorium adenophorum Spreng. J Chem Ecol 20:1885–1892CrossRefGoogle Scholar
  3. Bertin C, Weston LA, Huang T, Jander G, Owens T, Meinwald J, Schroeder FC (2007) Grass roots chemistry: meta-Tyrosine, an herbicidal nonprotein amino acid. Proc Natl Acad Sci USA 104:16964–16969PubMedCrossRefGoogle Scholar
  4. Canuto KM, Lima MA, Silveira ER (2010) Amburosides C-H and 6-o-protocatechuoyl coumarin from Amburana cearensis. J Braz Chem Soc 21:1746–1753CrossRefGoogle Scholar
  5. Chon S-U, Kim J-D (2002) Biological activity and quantification of suspected allelochemicals from alfalfa plant parts. J Agron Crop Sci 188:281–285CrossRefGoogle Scholar
  6. Chon S-U, Choi S-K, Jung S, Jang H-G, Pyo B-S, Kim S-M (2002) Effects of alfalfa leaf extracts and phenolic allelochemicals on early seedling growth and root morphology of alfalfa and barnyard grass. Crop Prot 21:1077–1082CrossRefGoogle Scholar
  7. Chou CH, Patrick ZA (1976) Identification and phytotoxic activity of compounds produced during decomposition of corn and rye residues in soil. J Chem Ecol 2:369–387CrossRefGoogle Scholar
  8. Habib SA, Abdul Rahman AA (1988) Evaluation of some weed extracts against field dodder on alfalfa (Medicago sativa). J Chem Ecol 14:443–453CrossRefGoogle Scholar
  9. Li HH, Inoue M, Nishimura H, Mizutani J, Tsuzuki E (1993) Interactions of trans-cinnamic acid, its related phenolic allelochemicals, and abscisic acid in seedling growth and seed germination of lettuce. J Chem Ecol 19:1775–1787CrossRefGoogle Scholar
  10. Li YB, Xu H, Shi L, Li Z (2007) Allelopathic effects of Eupatorium adenophorum on five species of the family Gesneriaceae. Biodivers Sci 15:486–491CrossRefGoogle Scholar
  11. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  12. Ngoc TM, Lee I, Ha DT, Kim HJ, Min BS, Bae K (2009) Tyrosinase-inhibitory constituents from the twigs of Cinnamomum cassia. J Nat Prod 72:1205–1208PubMedCrossRefGoogle Scholar
  13. Sang WG, Zhu L, Axmacher JC (2010) Invasion pattern of Eupatorium adenophorum Spreng in Southern China. Biol Invasions 12:1721–1730CrossRefGoogle Scholar
  14. Sellami IH, Maamouri E, Chahed T, Wannes WA, Kchouk ME, Marzouk B (2009) Effect of growth stage on the content and composition of the essential oil and phenolic fraction of sweet marjoram (Origanum majorana L.). Ind Crop Prod 30:395–402CrossRefGoogle Scholar
  15. Tocquin P, Corbesier L, Havelange A, Pieltain A, Kurtem E, Bernier G, Perilleux C (2003) A novel high efficiency, low maintenance, hydroponic system for synchronous growth and flowering of Arabidopsis thaliana. BMC Plant Biol 3:2PubMedCrossRefGoogle Scholar
  16. Trounce B, Dyason R (2003) Agfacts—crofton weed. NSW Agriculture http://www.dpi.nsw.gov.au/__data/assets/pdf_file/0010/155962/crofton-weed.pdf. Accessed 12 June 2010
  17. Vega M, De Carvalho M, Vieira I, Braz-filho R (2008) Chemical constituents from the Paraguayan medicinal plant, Eupatorium macrocephalum Less. J Nat Med 62:122–123PubMedCrossRefGoogle Scholar
  18. Woo N, Badger M, Pogson B (2008) A rapid, non-invasive procedure for quantitative assessment of drought survival using chlorophyll fluorescence. Plant Methods 4:27PubMedCrossRefGoogle Scholar
  19. Yang GQ, Wan FH, Liu WX, Zhang XW (2006) Physiological effects of allelochemicals from leachates of Ageratina adenophora (Spreng.) on rice seedlings. Allelopathy J 18:237–246Google Scholar
  20. Yang GQ, Wan FH, Liu WX, Guo JY (2008) Influence of two allelochemicals from Ageratina adenophora Sprengel on ABA, IAA and ZR contents in roots of upland rice seedlings. Allelopathy J 21:253–262Google Scholar
  21. Zhang KM, Shi L, Jiang CD, Li ZY (2008) Inhibition of Ageratina adenophora on spore germination and gametophyte development of Macrothelypteris toresiana. J Integr Plant Biol 50:559–564PubMedCrossRefGoogle Scholar
  22. Zhao X (2009) Chemical constituents from Eupatorium adenophorum and their allelopathic effects. Master’s thesisGoogle Scholar
  23. Zhao X, Zheng GW, Niu XM, Li WQ, Wang FS, Li SH (2009) Terpenes from Eupatorium adenophorum and their allelopathic effects on Arabidopsis seeds germination. J Agric Food Chem 57:478–482PubMedCrossRefGoogle Scholar
  24. Zheng L, Feng YL (2005) Allelopathic effects of Eupatorium adenophorum Spreng. on seed germination and seedling growth in ten herbaceous species. Acta Ecol Sin 25:2782–2787Google Scholar

Copyright information

© Springer Basel AG 2012

Authors and Affiliations

  1. 1.The Germplasm Bank of Wild Species, Kunming Institute of BotanyChinese Academy of SciencesKunmingChina
  2. 2.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of BotanyChinese Academy of SciencesKunmingChina

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