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Polyketides from Neohelicosporium griseum: structure assignment and bioactivity investigation

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In the pursuit of discovering new active metabolites from helicosporous hyphomycetes, rice fermentation products of Neohelicosporium griseum were examined. Eight compounds were isolated from this saprophytic fungus, namely vertixanthone (1), diaportheone A (2), 1,3,6,8-tetrahydroxyanthraquinone (3), lecanoric acid (4), decarboxycitrinone (5), 6,8-dihydroxy-4-hydroxymethyl-3,5-dimethyl-isochromen-1-one (6), decarboxyhydroxycitrinone (7), and ergosterin (8). The 1D and 2D NMR characteristics of compound 1 in DMSO-d6 were detailed for the first time. Antimicrobial testing indicated that compounds 14 exhibited moderate activity against Pseudomonas aeruginosa, with compound 3 also showing weak activity against Staphylococcus aureus. In-vitro cytotoxicity assays revealed that compounds 1, 3, and 4 displayed cytotoxic activity against HELA cell lines, with respective IC50 values of 30.8, 13.7, and 14.1 µM. Compounds 1, 3, and 4 also showed significant cytotoxicity against A549 cell lines, with respective IC50 values of 24.7, 7.4, and 10.3 µM.

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LZ would like to express her gratitude to Mae Fah Luang University for providing a scholarship for her PhD studies. RSJ thanks Eminent Scholar offered by Kyun Hee University.


The National Natural Science Foundation of China (NSFC 32060013) and the Youth Science and Technology Talent Development Project from Guizhou Provincial Department of Education (QJHKYZ [2021]263 and QJHKYZ [2022]345) provided funding for this work.

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Correspondence to Yongzhong Lu or Ruvishika S. Jayawardena.

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Zhang, L., Ma, J., Yang, M. et al. Polyketides from Neohelicosporium griseum: structure assignment and bioactivity investigation. Med Chem Res 33, 308–313 (2024).

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