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A new triterpene diglycoside from the roots of Bupleurum chinense DC. and its inhibitory effect on adipogensis in 3T3-L1 cells

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Abstract

A new triterpene diglycoside, tibesaikosaponin V (TKV), was isolated from the methanol extract of the roots of Bupleurum chinense DC. by normal and reversed phase column chromatography. The chemical structure of this new compound was elucidated based on extensive spectroscopic analysis, including 1D and 2D NMR, HR-ESI-MS, and chemical degradation method. Our results indicated that TKV inhibited lipid accumulation and triacylglycerol content occurred without cytotoxicity to 3T3-L1 adipocytes. Furthermore, TKV significantly suppressed the mRNA expression of nuclear transcription factors such as, peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα). These results suggest that TKV shows the ability to inhibit 3T3-L1 preadipocyte differentiation of and may have therapeutic potential for obesity and its associated metabolic disorders.

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Acknowledgements

This work was financially supported by the West Light Foundation of Chinese Academy of Sciences (Y7C1031100), National Natural Science Foundation of China (21561142003, 31600281 and 41501262) Natural Science Foundation of Jiangsu Province (BK20171269), and Qing Lan Project of Jiangsu Province.

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Author notes

  1. These authors contributed equally: Yujing Feng, Zhou-Wei Wu, Yanyan Luo

Authors and Affiliations

  1. Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian, 223300, China

    Yujing Feng, Yanyan Luo, Liang Chen, Yufeng Cao, Xin-Feng Wang & Weicheng Hu

  2. Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China

    Zhou-Wei Wu, Lun Wang, Aftab Yaseen, Bin Chen, Ming-Kui Wang, Guo-Lin Zhang & Fu Li

  3. Department of Chemistry, Women University of Azad Jammu and Kashmir, Bagh, 12500, Pakistan

    Ashfaq Ahmad Khan

  4. Department of Gerontology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huanghe West Road, Huaian, 223300, China

    Xueqin Li

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  1. Yujing Feng
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Correspondence to Xin-Feng Wang, Fu Li or Weicheng Hu.

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Feng, Y., Wu, ZW., Luo, Y. et al. A new triterpene diglycoside from the roots of Bupleurum chinense DC. and its inhibitory effect on adipogensis in 3T3-L1 cells. Med Chem Res 28, 239–245 (2019). https://doi.org/10.1007/s00044-018-2279-5

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