Medicinal Chemistry Research

, Volume 27, Issue 5, pp 1538–1548 | Cite as

Evidences for the antitumor potentiality of Hemimycale arabica and Negombata magnifica mesohyls in hepatocellular carcinoma rat model

  • Hanaa H. Ahmed
  • Hanaa M. Rady
  • Soheir E. Kotob
Original Research


The current study was constructed to explore the antitumor activity of Hemimycale arabica (blue sponge) and Negombata magnifica (red sponge) mesohyls in rat model of HCC. Forty adult female albino rats were distributed into: (1) negative control group, (2) HCC group, (3) Doxorubcin (DOXO)-treated group, (4) mesohyl of H. arabica-treated group, and (5) mesohyl of N. magnifica-treated group. Serum AST, ALT, GGT, α-fucosidase (AFU) activities; bilirubin, AFP and CEA levels was evaluated. Histopathological investigation of liver tissue sections was performed. The findings of this study indicated the presence of significant enhancement in serum AST, ALT, GGT, AFU, bilirubin, AFP, and CEA values in HCC-afflicted rats vs. the control counterparts. Insignificant change in serum AST, ALT, and GGT activity as well as bilirubin levels has been detected upon treatment of HCC-afflicted rats with DOXO. DOXO treatment revealed significant depletion in AFU, CEA, and AFP serum values comparing with HCC-afflicted ones. Treatment of HCC-afflicted rats with mesohyl blue (Hemimycale arabica) or red (Negombata magnifica) sponges evoked significant blunting in the measured biochemical parameters with respect to the untreated HCC-afflicted ones. The favorable impact of the natural treatments was documented by the great recovery of the structural organization of the liver of the treated rats. The current study provides an experimental evidence for the antitumor potential of Hemimycale arabica and Negombata magnifyica mesohyls in HCC rat model which may be ascribed to the strong hepatoprotective activity, the powerful free radical scavenging ability, and the highly apoptotic property of their active constitutes.


Hepatocellular carcinoma Hemimycale arabica Negombata magnifica Mesohyl Rat 



The authors appreciate the project No. 10160003 entitled (Preclinical Evaluation of Anticancer Efficacy and Pharmacological Properties of Promising Marine Sponge—Derived Anticancer Agents) funded from the National Research Centre, Egypt, for its kind support and effort in performing this manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Authors and Affiliations

  • Hanaa H. Ahmed
    • 1
  • Hanaa M. Rady
    • 2
  • Soheir E. Kotob
    • 1
  1. 1.Hormones DepartmentNational Research CentreGizaEgypt
  2. 2.Chemistry of Natural Compounds DepartmentNational Research CentreGizaEgypt

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