Medicinal Chemistry Research

, Volume 27, Issue 5, pp 1449–1459 | Cite as

The protective effects of MPLC isolated glycyrrhizin and mangiferin against brain toxicity in rats

  • Sylvia A. Boshra
  • Alaadin E. El-Haddad
Original Research


The present study was conducted to demonstrate a method for preparative isolation of glycyrrhizin and mangiferin using medium-pressure liquid chromatography (MPLC) and to further investigate their protective effects against aluminum-induced brain toxicity in rats compared to melatonin (classical neuroprotective drug). Brain toxicity was determined through assessment of lipid profile, B-cell lymphoma 2 (Bcl2), Bcl-2-associated X protein (Bax), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), lipid peroxide (malondialdehyde), nitric oxide (NO), cytokines level such as tumor necrosis factor-alpha (TNF-α) and interleukins (IL-22 and IL-1β), as well as gene expression of brain SOD, CAT, and IL-22. Glycyrrhizin and mangiferin were isolated employing reverse phase MPLC and their structures were confirmed using NMR spectroscopy. Glycyrrhizin and/or mangiferin alone or in combination, as well as melatonin co-treatment prior to AlCl3 entoxication in rats revealed significant amelioration in all biochemical parameters relevant to brain function. Enhanced brain functions in AlCl3 intoxicated rats was attributed to a mechanism related to their antioxidant and anti-inflammatory potential.


Glycyrrhizin Mangiferin Melatonin Brain neurotoxicity AlCl3 toxicity 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Authors and Affiliations

  1. 1.Biochemistry Department, Faculty of PharmacyOctober 6 UniversityGizaEgypt
  2. 2.Pharmacognosy Department, Faculty of PharmacyOctober 6 UniversityGizaEgypt

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