Screening and isolating potential α-glucosidase inhibitors from Rhizoma Coptidis by ultrafiltration LC-PDA-ESI/MS combined with high-speed countercurrent chromatography and reverse-phase medium-pressure liquid chromatography
Rhizoma Coptidis is an alkaloid-rich herbal drug used in Chinese herbal medicine. Currently, conventional methods for screening and isolating alkaloids are labor-intensive and time-consuming. In the present study, ultrafiltration liquid chromatography with photodiode array detection coupled to electrospray ionization tandem mass spectrometry (ultrafiltration liquid chromatography-photodiode array detector-electrospary ionization mass spectrometry (LC-PDA-ESI/MS)) were applied to screen and identify α-glucosidase inhibitors in R. Coptidis. High-speed countercurrent chromatography and reverse-phase medium-pressure liquid chromatography were applied to separate and isolate the active constituents. As a result, five major compounds in R. Coptidis were screened and identified as α-glucosidase inhibitors by ultrafiltration LC-PDA-ESI/MS. Five ligands, jatrorrhizine, epiberberine, coptisine, palmatine, and berberine, were isolated by reverse-phase medium-pressure liquid chromatography and high-speed countercurrent chromatography. The purities of the five compounds as determined by high-performance liquid chromatography were 76.20, 75.13, 82.24, 93.78, and 92.01%, respectively. The results indicate that systematic isolation of bioactive components in R. Coptidis guided by ultrafiltration LC-PDA-ESI/MS is a feasible and efficient technique that could be extended to separation of other enzyme inhibitors.
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This work was supported by the National Natural Science Foundation of China (No. 31170326, 31370374), the team project of Jilin Provincial Science and Technology Department (No. 20130413043GH), the Academic Innovation Foundation of Changchun Normal University (cscxy2015003), the project of Jilin Provincial Education Department ( 253), and the Natural Science Foundation of Changchun Normal University (No.  001).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
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