Medicinal Chemistry Research

, Volume 26, Issue 12, pp 3091–3105 | Cite as

Albizia harveyi: phytochemical profiling, antioxidant, antidiabetic and hepatoprotective activities of the bark extract

  • Mansour Sobeh
  • Mona F. Mahmoud
  • Mohamed A. O. Abdelfattah
  • Hesham A. El-Beshbishy
  • Assem M. El-Shazly
  • Michael Wink
Original Research

Abstract

Profiling the polyphenols in the methanol extract from the bark of Albizia harveyi was performed by HPLC–PDA–ESI–MS/MS analysis. The phytochemical analysis identified 39 compounds, the majority of them were flavan-3-ol derivatives and condensed tannins. Total phenolic content, determined by the Folin–Ciocalteu method amounted to 489 mg gallic acid equivalents/g extract. The extract showed promising antioxidant activities with an EC50 of 3.6 µg/mL and 18.32 mM FeSO4 equivalent/mg extract in radical scavenging assay and ferric reducing antioxidant power assays, respectively. The hepatoprotective potential of the extract in rats was determined in vivo in a d-galactosamine-induced liver toxicity model. A dose of 100 mg/kg (body weight) of the bark extract reduced levels of aspartate aminotransferase, gamma-glutamyltransferase and total bilirubin by 35.7, 65.3, and 23.8% (p < 0.05), respectively whereas glutathione was increased by 59.1%. These effects were similar to silymarin which was used as positive control. The extract (100 mg/kg (body weight) mediated a substantial antidiabetic response in streptozotocin-induced diabetic rats manifested by a significant reduction in serum glucose and lipid peroxides and significant increase of serum insulin. Docking of d-(+) catechin and the dimer (epi)catechin-(epi)catechin into the active site of the enzymes human pancreatic α-amylase, maltase-glucoamylase, and aldol reductase revealed that these enzymes may be possible targets via which, the studied Albizia harveyi extract could exert its antidiabetic effect.

Keywords

HPLC–PDA–ESI–MS/MS Antioxidant activity Hepatoprotective activity Antidiabetic activity Molecular modeling Albizia harveyi 

Notes

Acknowledgements

The authors would like to thank Dr. C. D. Rubanza (Tanzania Forestry Research Institute, Shinyanga, Tanzania) for plant collection and Dr. B. Wetterauer (IPMB) for his help in collecting LC-MS data.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Mansour Sobeh
    • 1
  • Mona F. Mahmoud
    • 2
  • Mohamed A. O. Abdelfattah
    • 3
  • Hesham A. El-Beshbishy
    • 4
    • 5
  • Assem M. El-Shazly
    • 6
  • Michael Wink
    • 1
  1. 1.Institute of Pharmacy and Molecular Biotechnology, Heidelberg UniversityHeidelbergGermany
  2. 2.Department of Pharmacology and Toxicology, Faculty of PharmacyZagazig UniversityZagazigEgypt
  3. 3.Department of ChemistryAmerican University of the Middle EastEqailaKuwait
  4. 4.Medical Laboratory Sciences DepartmentFakeeh College for Medical SciencesJeddahSaudi Arabia
  5. 5.Biochemistry Department, Faculty of PharmacyAl-Azhar UniversityCairoEgypt
  6. 6.Department of Pharmacognosy, Faculty of PharmacyZagazig UniversityZagazigEgypt

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