Medicinal Chemistry Research

, Volume 26, Issue 10, pp 2410–2419 | Cite as

A fast affinity extraction methodology for rapid screening of bioactive compounds specifically binding to beta2-adrenergic receptor from Xie-Bai-San

  • Zhenyu Sun
  • Jing Wang
  • Qian Li
  • Meimei Zhao
  • Yajun Zhang
  • Xunyu Xiong
  • Xinfeng Zhao
  • Xiaohui Zheng
Original Research


Despite promising progress in series of techniques for drug discovery, the number of approved drugs has decreased in recent decades due to the lack of high efficient methods for candidate screening. In this work, immobilized beta2-adrenergic receptor was utilized to develop a new method for screening bioactive compounds from complex prescription, involving oriented immobilization of beta2-adrenergic receptor through diazonium reaction, the fast affinity extraction of bioactive compounds from Xie-Bai-San and the identification of these compounds by time of flight mass spectrometry. Valine, 4-hydroxyl resveratrol and kukoamine B were identified as the bioactive compounds specifically binding to beta2-adrenergic receptor in Xie-Bai-San. The binding of these compounds to the receptor occurred on four amino acid residues, viz., Asp 113, Ser 204, Ser 207, and Phe 290 in the crystal structure of beta2-adrenergic receptor. Hydrogen bond and hydrophobic force were believed to drive the binding of the compounds to beta2-adrenergic receptor on these residues. The results indicated that the proposed methodology has advantages on rapid, high throughput, and specific analysis, meanwhile, it has the capacity to probe the binding sites of the screened bioactive compounds. Fast affinity extraction methodology is expected to become a powerful alternative, which is superior in rapid screening of bioactive compounds from complex matrices such as traditional Chinese medicine.


Beta2-adrenergic receptor Xie-Bai-San Fast affinity extraction Bioactive compounds 



The authors greatly thank the finacial support from the Scientific Research Plan Projects of Shaanxi Education Department (No. 14JK1571), the Natural Science Basic Research Plan in Shaanxi Province of China (Nos 2015JQ5069, 2016JM8057), the program for Innovative Research Team of Shaanxi Province (No. 2013KCT-24), the Ministry of Science and Technology of the People’s Republic of China (No. 2013YQ170525; subproject: 2013YQ17052509), the National Natural Science Foundation of China (No. 21475103), the Natural Science Foundation of Shaanxi Province (No. 2015JM2072), the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT_15R55) for the financial support. The innovation and entrepreneurship training program of Northwest University (2016179).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Zhenyu Sun
    • 1
  • Jing Wang
    • 1
  • Qian Li
    • 1
  • Meimei Zhao
    • 1
  • Yajun Zhang
    • 1
  • Xunyu Xiong
    • 2
  • Xinfeng Zhao
    • 1
  • Xiaohui Zheng
    • 1
  1. 1.Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life SciencesNorthwest UniversityXi’anChina
  2. 2.College of Chemistry and Chemical EngineeringXi’an Shiyou UniversityXi’anChina

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